Two kinds of lateral flow immunoassays based on multifunctional magnetic prussian blue nanoenzyme and colloidal gold for the detection of 38 β-agonists in swine urine and pork

Liang, Jinxuan; Liu, Zhiwei; Fang, Yalin; Shen, Xing; Xu, Zhen-Lin; Lei, Hongtao; Huang, Xianhui; Li, Xiangmei

doi:10.1016/j.foodchem.2023.135897

Cited by 20 publications

(11 citation statements)

References 40 publications

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“…The standard curves of DXMS were established based on the B / B 0 against the DXMS concentration, where B and B 0 were the ratios of T / C values with and without competitive Ag in the sample solutions, respectively . The limit of detection (LOD) was defined as the average of 20 blank samples plus three times the standard deviation …”

Section: Resultsmentioning

confidence: 99%

“…31 The limit of detection (LOD) was defined as the average of 20 blank samples plus three times the standard deviation. 32 Under natural light, the color signals gradually "turned off" as the analyte concentration increased, with vLODs of 0.20, 0.50, and 0.50 μg/kg for milk, beef, and pork, respectively (Figure 3A−C), while the fluorescence signals gradually "turned on" under 365 nm UV excitation, with vLODs of 0.20, 0.35, and 0.35 μg/kg for three samples, respectively (Figure 3D−F). The LODs of Ti 3 C 2 @Au-mediated dual-signal immunosensor in colorimetric mode were 0.0018, 0.12, and 0.084 μg/kg, and the linear ranges were 0.05−0.80, 0.35−1.50, and 0.25−1.00 μg/kg, respectively, whereas in fluorescence mode, the LODs were 0.0013, 0.080, and 0.070 μg/kg, and the linear ranges were 0.05−0.80, 0.35−2.00, and 0.25−1.50 μg/kg for milk, beef, and pork, respectively.…”

Section: Analyticalmentioning

confidence: 99%

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High-Density Au Anchored to Ti₃C₂-Based Colorimetric-Fluorescence Dual-Mode Lateral Flow Immunoassay for All-Domain-Enhanced Performance and Signal Intercalibration

Deng,

Wang,

Lin

et al. 2024

Anal. Chem.

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In this work, a novel MXene-Au nanoparticle (Ti 3 C 2 @ Au) was synthesized with a high molar extinction coefficient, strong fluorescence quenching ability, ultrahigh antibody affinity, high stability, and good dispersibility, and it was used to develop a colorimetric-fluorescence dual-mode lateral flow immunoassay (LFIA). The detection limits of this method for the detection of dexamethasone in milk, beef, and pork were 0.0018, 0.12, and 0.084 μg/kg in the "turn-off" mode (colorimetric signal), and 0.0013, 0.080, and 0.070 μg/kg in the "turn-on" mode (fluorescent signal), respectively, which was up to 231-fold more sensitive compared with that of the reported LFIAs. The recovery rates ranged from 81.1− 113.7%, and 89.2−115.4%, with the coefficients of variation ranging from 1.4−15.0%, and 1.9−14.8%, respectively. The results of the LC-MS/MS confirmation test on 30 real samples had a good correlation with that of our established method (R 2 > 0.97). This work not only developed novel nanocarriers for antibody-based LFIA but also ensured high-performance detection.

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Section: Resultsmentioning

confidence: 99%

Section: Analyticalmentioning

confidence: 99%

High-Density Au Anchored to Ti₃C₂-Based Colorimetric-Fluorescence Dual-Mode Lateral Flow Immunoassay for All-Domain-Enhanced Performance and Signal Intercalibration

Deng,

Wang,

Lin

et al. 2024

Anal. Chem.

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“…Nanozymes can be conjugated with functional molecules by physical adsorption or covalent binding [ 193 ]. Physical adsorption is arguably the easiest method for the conjugation; it was used for binding antibodies to Pt nanoparticles [ 194 ], Pt-coated GNPs [ 195 ], Fe 3 O 4 coated with polydopamine [ 196 ], VS 2 nanosheets [ 197 ], and Prussian blue nanoparticles [ 198 ]. Covalent coupling uses bi-functional crosslinkers (usually containing sulfhydryl and carboxyl groups) and reagents for carbodiimide chemistry [ 199 , 200 , 201 ].…”

Section: Post-assay Chemical Enhancement Approachesmentioning

confidence: 99%

Post-Assay Chemical Enhancement for Highly Sensitive Lateral Flow Immunoassays: A Critical Review

Panferov,

Zherdev,

Dzantiev

2023

Biosensors

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Lateral flow immunoassay (LFIA) has found a broad application for testing in point-of-care (POC) settings. LFIA is performed using test strips—fully integrated multimembrane assemblies containing all reagents for assay performance. Migration of liquid sample along the test strip initiates the formation of labeled immunocomplexes, which are detected visually or instrumentally. The tradeoff of LFIA’s rapidity and user-friendliness is its relatively low sensitivity (high limit of detection), which restricts its applicability for detecting low-abundant targets. An increase in LFIA’s sensitivity has attracted many efforts and is often considered one of the primary directions in developing immunochemical POC assays. Post-assay enhancements based on chemical reactions facilitate high sensitivity. In this critical review, we explain the performance of post-assay chemical enhancements, discuss their advantages, limitations, compared limit of detection (LOD) improvements, and required time for the enhancement procedures. We raise concerns about the performance of enhanced LFIA and discuss the bottlenecks in the existing experiments. Finally, we suggest the experimental workflow for step-by-step development and validation of enhanced LFIA. This review summarizes the state-of-art of LFIA with chemical enhancement, offers ways to overcome existing limitations, and discusses future outlooks for highly sensitive testing in POC conditions.

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“…As mentioned above, PBNPs are the multifunctional nanomaterials with magnetic, colorimetric, and catalytic properties in one, which will facilitate the improvement of LFIA performance in multiple dimensions. Liang et al [77] ingeniously modified PBNPs on the surface of MNPs, significantly enhancing the color intensity and enzyme catalytic activity, while also retaining their strong magnetism. It can be used for the detection of 38 kinds of β-agonists in pig urine and pork (Figure 10c).…”

Section: Lateral Flow Immunoassaysmentioning

confidence: 99%

mentioning

confidence: 99%

Advances in nanoprobes‐based immunoassays

Zhang,

Huang,

Jin

et al. 2023

BMEMat

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Immunoassay is a powerful technique that uses highly specific antigen‐antibody interactions to detect biochemical targets such as proteins and toxins. As a diagnostic tool, immunoassay is employed in the screening, diagnosis, and prognosis of diseases, which are crucial for the grasp and control of patient conditions in clinical practice. With the rapid development of nanotechnology, immunoassays based on nanoprobes have attracted more and more attention due to the advantages of high sensitivity, specificity, stability, and versatility. These nanoprobes are nanoscale particles that can act as signal carriers or targeting agents for immunoassays. In this paper, we review the recent advances in various types of nanoprobes for immunoassays, such as colloidal gold, quantum dots, magnetic nanoparticles, nanozymes, aggregation‐induced emission, and up‐conversion nanoparticles. The effect of the nanoprobe construction and synthesis methods on their detection performance deserves to be studied in depth. We also compare their detection ranges and limits in different immunoassay methods, such as lateral flow immunoassays, fluorescent immunoassays, and surface‐enhanced Raman scattering immunoassays. Moreover, we discuss the benefits and challenges of nanoprobes in immunoassays and provide insights into their future development. This study aims to offer a comprehensive and critical perspective on the role of nanoprobes in the field of immunoassays.

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Two kinds of lateral flow immunoassays based on multifunctional magnetic prussian blue nanoenzyme and colloidal gold for the detection of 38 β-agonists in swine urine and pork

Cited by 20 publications

References 40 publications

High-Density Au Anchored to Ti₃C₂-Based Colorimetric-Fluorescence Dual-Mode Lateral Flow Immunoassay for All-Domain-Enhanced Performance and Signal Intercalibration

High-Density Au Anchored to Ti₃C₂-Based Colorimetric-Fluorescence Dual-Mode Lateral Flow Immunoassay for All-Domain-Enhanced Performance and Signal Intercalibration

Post-Assay Chemical Enhancement for Highly Sensitive Lateral Flow Immunoassays: A Critical Review

Advances in nanoprobes‐based immunoassays

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Two kinds of lateral flow immunoassays based on multifunctional magnetic prussian blue nanoenzyme and colloidal gold for the detection of 38 β-agonists in swine urine and pork

Cited by 20 publications

References 40 publications

High-Density Au Anchored to Ti3C2-Based Colorimetric-Fluorescence Dual-Mode Lateral Flow Immunoassay for All-Domain-Enhanced Performance and Signal Intercalibration

High-Density Au Anchored to Ti3C2-Based Colorimetric-Fluorescence Dual-Mode Lateral Flow Immunoassay for All-Domain-Enhanced Performance and Signal Intercalibration

Post-Assay Chemical Enhancement for Highly Sensitive Lateral Flow Immunoassays: A Critical Review

Advances in nanoprobes‐based immunoassays

Contact Info

Product

Resources

About

High-Density Au Anchored to Ti₃C₂-Based Colorimetric-Fluorescence Dual-Mode Lateral Flow Immunoassay for All-Domain-Enhanced Performance and Signal Intercalibration

High-Density Au Anchored to Ti₃C₂-Based Colorimetric-Fluorescence Dual-Mode Lateral Flow Immunoassay for All-Domain-Enhanced Performance and Signal Intercalibration