Two iridovirus isolates from Carassius auratus (L.)

Abstract

Two new viruses, goldfish virus-1 (GFV-1) and goldfish virus-2 (GFV-2), were isolated from swimbladder cultures prepared from healthy Carassius auratus (L.). The isolates were sensitive to pH, heat, and chloroform but stable to freezing and thawing. Treatment with IUDR, Feulgen staining and acridine orange staining indicated that the viruses contain double-stranded DNA. Electron microscopy revealed enveloped virions of 180 nm enclosing icosahedral capsids of 120 nm. Viral growth was studied by light and electron microscopy and infectivity assays. Virions were assembled in cytoplasmic inclusion bodies and apparently released through cell degeneration. Infectious virus was first produced at 24 h post-infection. Progressive cytopathology was observed leading to cell ghosts by 96 h post-infection. The results suggest tentative placement of the viruses in the family Iridoviridae.

Replication and pathogenesis of white sturgeon iridovirus (WSIV) in experimentally infected white sturgeon Acipenser transmontanus juveniles and sturgeon cell lines

“…These agents possess morphological, biochemical and serological characteristics in common with the amphibian iridovirus FV-3 , Hedrick et al 1992a, Hengstberger et al 1993 but not with either WSIV or FDLV. These systemic agents, including EHNV, sheatfish iridovirus and red sea bream lridovirus (RSIV), proliferate rapidly over a broad range of temperatures and cell lines (Berry et al 1983, Langdon et al 1986, Ogawa et al 1990, Nakajima et al 1995. Rapid cell death and budding from plasma membranes allows viral transmission from infected cells and infected hosts (Hengstberger et al 1993).…”

An iridovirus-like agent isolated from the ornate burrowing frog Limnodynastes ornatus in northern Australia

“…A number of iridoviruses have been found in fish, but only 4, fish lymphocystis disease virus (FLDV), EHNV, goldfish virus (GFV) and sheatfish virus, appear to have been isolated and characterised (Berry et al 1983, Langdon & Humphrey 1987, Ahne et al 1989. FLDV is distinct from the ranaviruses, GFV and EHNV (Essani & Granoff 1989, Eaton et al 1991.…”

Isolation and Characterization of a Pathogenic Iridovirus from Cultured Grouper (Epinephelus sp.) in Taiwan.

“…These include lymphocystis virus (Wolf et al, 1966), erythrocytic necrosis virus (Appy et al, 1976), cod iridovirus (Jensen et al, 1979), goldfish iridovirus (Berry et al, 1983), carp gill necrosis iridovirus (Shchelkunov and Shchelkunova, 1984), redfin perch iridovirus (Langdon et al, 1986(Langdon et al, , 1988Langdon and Humphrey, 1987) and sheatfish iridovirus (Ahne et al, 1991). In Singapore, an iridovirus has been implicated in a novel disease, "Sleepy Grouper Disease" in brownspotted grouper Epinephelus tauvina (Chua et al, 1994).…”