Two insulin receptors coordinate oogenesis and oviposition via two pathways in the green lacewing, Chrysopa pallens

Han, Benfeng; Zhang, Tingting; Feng, Yanjiao; Liu, Xiaopin; Zhang, Lisheng; Chen, Hongyin; Zeng, Fanrong; Wang, Mengqing; Liu, Chenxi; Li, Yuyan; Cui, Jinjie; Li, Zhaoqun; Mao, Jianjun

doi:10.1016/j.jinsphys.2020.104049

Cited by 27 publications

(33 citation statements)

References 41 publications

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“…The fecundity and egg hatchability were significantly reduced after knockdown of LsAkt expression. Similar defective phenotypes were observed after RNAi of Akt in C. pallens (Han et al, 2020). Depletion of Akt may have inhibited Vg synthesis and ovarian growth, thereby reducing the fecundity of L. serricorne.…”

Section: Discussionsupporting

confidence: 58%

“…Bioassay analysis by sparing with a mixture of transformed Escherichia coli expressing dsAkt and Bacillus thuringiensis (Bt) in fourth-instar larvae of M. vitrata produced higher control mortality than a single treatment with Bt alone or dsAkt alone (Al Baki et al, 2020). In Chrysopa pallens, knockdown of Akt suppressed the expression of the vitellogenin (Vg) gene, hampered ovarian development, and reduced egg mass and hatching rate (Han et al, 2020). By contrast, overexpression of active Akt in the fat body of Anopheles stephensi extended the lifespan and increased fecundity of females (Hun et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Disruption of the Serine/Threonine Kinase Akt Gene Affects Ovarian Development and Fecundity in the Cigarette Beetle, Lasioderma serricorne

Yan

et al. 2021

Front. Physiol.

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Serine/threonine kinase Akt, an important component of the insulin signaling pathway, plays an essential role in many physiological processes. In this study, we identified and characterized an Akt gene (designated LsAkt) from the cigarette beetle, Lasioderma serricorne. LsAkt contains a 1614 bp open reading frame encoding a 537 amino acid protein that possesses a conserved pleckstrin homology domain and a serine/threonine kinase domain. The expression of LsAkt was high in pupal stages and peaked in day-4 female pupae. In adult tissues, LsAkt was highly expressed in the thorax, ovary, and midgut. The expression of LsAkt was induced by methoprene or bovine insulin in vivo, but significantly decreased by 20-hydroxyecdysone. RNA interference-mediated knockdown of LsAkt resulted in severely blocked ovarian development and reduced fecundity and hatchability. The vitellogenin (Vg) content and juvenile hormone (JH) titers of LsAkt-depletion beetles were decreased, and expressions of Vg and four JH signaling and biosynthetic genes were significantly decreased. Silencing of LsAkt reduced the amounts of glucose, glycogen, and trehalose in female adults and affected the expressions of seven key carbohydrate metabolic genes. Taken together, it is inferred that Akt implicates in L. serricorne reproduction by modification of Vg synthesis, juvenile hormone production and carbohydrate metabolism.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Disruption of the Serine/Threonine Kinase Akt Gene Affects Ovarian Development and Fecundity in the Cigarette Beetle, Lasioderma serricorne

Yan

et al. 2021

Front. Physiol.

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“…Furthermore, in addition to functional discrepancy, multiple InR paralogues may have overlapping functions on some aspects of life-history traits in some insects. Knockdown of either two InR paralogues impaired fecundity in the green lacewing Chrysopa pallens [ 32 ] and red flour beetle Tribolium castaneum [ 33 ], and disrupted nymph-adult transition in the brown citrus aphid Aphis ( Toxoptera ) citricidus [ 34 ]. These evidences raise an intriguing question regarding to the extent of functional conservation between multiple InR paralogues in insects.…”

Section: Introductionmentioning

confidence: 99%

Neofunctionalization of a second insulin receptor gene in the wing-dimorphic planthopper, Nilaparvata lugens

Xue

Chen

et al. 2021

PLoS Genet

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A single insulin receptor (InR) gene has been identified and extensively studied in model species ranging from nematodes to mice. However, most insects possess additional copies of InR, yet the functional significance, if any, of alternate InRs is unknown. Here, we used the wing-dimorphic brown planthopper (BPH) as a model system to query the role of a second InR copy in insects. NlInR2 resembled the BPH InR homologue (NlInR1) in terms of nymph development and reproduction, but revealed distinct regulatory roles in fuel metabolism, lifespan, and starvation tolerance. Unlike a lethal phenotype derived from NlInR1 null, homozygous NlInR2 null mutants were viable and accelerated DNA replication and cell proliferation in wing cells, thus redirecting short-winged–destined BPHs to develop into long-winged morphs. Additionally, the proper expression of NlInR2 was needed to maintain symmetric vein patterning in wings. Our findings provide the first direct evidence for the regulatory complexity of the two InR paralogues in insects, implying the functionally independent evolution of multiple InRs in invertebrates.

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“…The IR is also indispensable in insect photoperiodism, lifespan, and aging due to its relation to metabolism and growth [ 25 , 91 , 92 , 93 ]. Overall, studies have indicated that the IR is indispensable in insect growth [ 94 ], development and reproduction [ 95 , 96 ], polymorphism [ 24 ], lifespan [ 97 ], and oviposition [ 98 ]. Therefore, the IR represents an important target for the management of pests and parasites.…”

Section: Functions Of the Irmentioning

confidence: 99%

The Insulin Receptor: An Important Target for the Development of Novel Medicines and Pesticides

Zhang

Zhu

et al. 2022

IJMS

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The insulin receptor (IR) is a transmembrane protein that is activated by ligands in insulin signaling pathways. The IR has been considered as a novel therapeutic target for clinical intervention, considering the overexpression of its protein and A-isoform in multiple cancers, Alzheimer’s disease, and Type 2 diabetes mellitus in humans. Meanwhile, it may also serve as a potential target in pest management due to its multiple physiological influences in insects. In this review, we provide an overview of the structural and molecular biology of the IR, functions of IRs in humans and insects, physiological and nonpeptide small molecule modulators of the IR, and the regulating mechanisms of the IR. Xenobiotic compounds and the corresponding insecticidal chemicals functioning on the IR are also discussed. This review is expected to provide useful information for a better understanding of human IR-related diseases, as well as to facilitate the development of novel small-molecule activators and inhibitors of the IR for use as medicines or pesticides.

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Two insulin receptors coordinate oogenesis and oviposition via two pathways in the green lacewing, Chrysopa pallens

Cited by 27 publications

References 41 publications

Disruption of the Serine/Threonine Kinase Akt Gene Affects Ovarian Development and Fecundity in the Cigarette Beetle, Lasioderma serricorne

Disruption of the Serine/Threonine Kinase Akt Gene Affects Ovarian Development and Fecundity in the Cigarette Beetle, Lasioderma serricorne

Neofunctionalization of a second insulin receptor gene in the wing-dimorphic planthopper, Nilaparvata lugens

The Insulin Receptor: An Important Target for the Development of Novel Medicines and Pesticides

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