Two Host-Inducible Genes of
Rhizobium fredii
and Characterization of the Inducing Compound
Abstract:Random transcription fusions with Mu dl(Kan lac) generated three mutants in Rhizobium fredii (strain USDA 201) which showed induction of I-galactosidase when grown in root exudate of the host plants Glycine max, Phaseolus vulgaris, and Vigna ungliculata. Two genes were isolated from a library of total plasmid DNA of one of the mutants, 3F1. These genes, present in tandem on a 4.2-kilobase Hindlll fragment, appear in one copy each on the symbiotic plasmid and do not hybridize to the Rhizobium meliloti common no… Show more
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