Two histone H1-encoding genes of the green alga Volvox carteri with features intermediate between plant and animal genes

Lindauer, Andreas; Müller, Kurt; Schmitt, Rüdiger

doi:10.1016/0378-1119(93)90696-z

Cited by 20 publications

(18 citation statements)

References 43 publications

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“…We found that the histone H1 mRNA is a polyadenylated message (data not shown) and, in this respect, it is similar to all the other reported higher plant H1 mRNAs [9,10,21,291. In contrast, the Volvox [20] and most animal cell histone H1 mRNAs are non-polyadenylated, and those which are generally fit into the category of developmental variants or replication-independent variants. Messenger RNAs encoding core histones in plants have also been shown to be polyadenylated [4,19,27,281, again in contrast to the animal core histone mRNAs.…”

Section: Speciesmentioning

confidence: 99%

“…Whether this will become common among higher plants must await the characterization of other H1 genomic clones. The histone H1 genes of the green alga Volvox [20] contain three introns and, in one of the two genes, the po$ition of the first intron corresponds to that found in the tomato and Arubidopsis genes. Thus, from the very limited data set, it would appear that plants have retained their introns whereas animals, in the majority of cases, have lost them from H1 genes.…”

Section: Some Notes On Evolutionmentioning

confidence: 99%

“…Hexamer repeat motifs in the carboxy-terminal domains of plant histone H1 molecules. Hexamer peptide consensus sequences derived from repeats in the carboxy-terminal domains of histones H1 from tomato, A. thaliana HI-1 [lo], maize [21], wheat [29], pea [9] and Volvox HI-I1 [20]. The numbers correspond to the number of repeats that match the consensus.…”

Section: Expression Of Histone H1 Mrna In Various Organsmentioning

confidence: 99%

“…However, in higher plants, only two monocot (wheat [29] and maize [21]) and two dicot (pea [9] and Arubidopsis [lo]) histone H1 genes have been cloned and sequenced. Recently, two H1 genes from the green alga Volvox have been described [20]. The Arubidopsis and Vulvox reports represent the only plant genomic clones reported to date.…”

mentioning

confidence: 99%

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Molecular cloning, sequence analysis and differential expression of an intron‐containing gene encoding tomato histone H1

Jayawardene

Riggs

1994

European Journal of Biochemistry

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A tomato genomic clone has been identified which encodes histone H1. The deduced polypeptide is 287 amino acids in length, and exhibits the tripartite organization typical of histones H1. The central globular domain is highly similar to those regions from other H1 molecules, and the carboxyl-terminal domain contains a repeating hexapeptide motif, variants of which are conserved among H1 molecules. RNA gel blotting revealed that histone H1 mRNA is expressed at higher levels in organs which contain meristematic tissue and/or which have a high proportion of actively cycling cells. DNA gel blotting and dot-blot hybridization studies revealed that histone H1 in tomato is encoded by a small gene family. By employing the polymerase chain reaction on genomic DNA and on cDNA, it was determined that the gene is interrupted by an intron. The location and approximate length of the intron are conserved in both the tomato and Arubidopsis genes, with the intron separating the 'nose' region (encoded by exon 1) from the central globular domain (exon 2). The promoter region was found to contain several conserved sequence motifs which likely participate in the regulation of the gene.In most eukaryotic cells, the most elementary level of chromatin packaging is accomplished by the interaction of two molecules of each of the core histones H2A, H2B, H3 and H4 with 146-bp of duplex DNA to yield the core particle of the nucleosome. Adjacent core particles are connected by a linker DNA region of approximately 40-60 bp and, on average, there exists one molecule of histone Hlhucleosome. While the sequences of the core histones are well conserved between widely divergent species, histone H1 molecules exhibit striking sequence divergence. Despite this divergence, histone H1 molecules have maintained three distinct regions. These consist of an amino-terminal 'nose', a conserved central globular domain and a positively charged carboxy-terminal tail. One role of histone H1 is to act at a second level of the hierarchy of chromatin packaging to direct the formation and maintenance of the 30-nm chromatin fiber. It is generally thought that the positively charged lysine residues, found predominantly in the tail region, neutralize the negative phosphates of linker DNA to permit compaction of the chromatin fiber. Several lines of evidence suggest that the conserved central globular domain is responsible for sealing the points of entry and exit of DNA from the core particle [7]. In the central globular domain of histone H1 molecules from both plants and animals, there are many conserved resi-

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Section: Speciesmentioning

confidence: 99%

Section: Some Notes On Evolutionmentioning

confidence: 99%

Section: Expression Of Histone H1 Mrna In Various Organsmentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular cloning, sequence analysis and differential expression of an intron‐containing gene encoding tomato histone H1

Jayawardene

Riggs

1994

European Journal of Biochemistry

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“…2). Interestingly, the Lys-Ala-Pro repeat is also found inserted, at almost the same distance from a Lys-Ala-Ala sequence and from the conserved region in the central domain, in the deduced amino acid sequence of one of the two known Hi genes of the alga V. carteri (21). Here it has the sequence Lys-Ala-Pro-Lys-Gln-Pro-Lys-Ala-ProLys-Ala-Pro and is flanked by additional Lys-Ala-Pro elements (Fig.…”

Section: Resultsmentioning

confidence: 99%

Structural and functional differences between histone H1 sequence variants with differential intranuclear distribution.

Schulze

Trieschmann

Schulze

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

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The chromatin of most cell types contains several different sequence variants of histone Hi. The functional role of this heterogeneity is not known. In the larval tissues ofthe midge, Chironomus thummi, there are Hi variants of two types. Hi 11-1, Hi 11-2, and Hi rI-1 have similar amino acid sequences and appear uniformly distributed in polytene interphase chromosomes. The total number of gene copies per genome for this type of Hi histones is about 40 inC. th. thummi and 50-60 in C. th. piger. In contrast, histone Hi I-1 is encoded by a single copy gene in C. th. thummi and by two to four genes in C. th. piger. It has a divergent structure and is found only in a limited number of condensed chromosome sites. The N-terminal domain of Hi I-1 contains an insertion that is lacking in the other Hi variants and that is part of a variantspecific bipartite sequence Lys-Ala-Pro-Lys-Ala-Pro-Xaal0- Chironomus thummi. Several of the Hi variants of this species appear to be present throughout the polytene chromosomes, whereas Hi I-1, the variant with the most diverging structure (9), was detected only in a limited number of chromosome bands. Many of these bands replicate late in S-phase (10) and contain repetitive DNA (11, 12). They are more frequent in C. th. thummi than in C. th. piger. Accordingly, Hi I-1 was found to account for about 20%o of total Hi in larvae of C. th. piger and for about 30% in larvae of C. th. thummi (8).The demonstration within one nucleus of differences between chromosome sites in their Hi subtype content makes C. thummi a model organism for studying possible functions of Hi heterogeneity. Therefore, we have determined and compared the sequences of the different Hi histone genes of C. thummi in a search for structural features that are peculiar to Hi I-1 and could be involved in establishing a particular subtype of chromatin. We find that Hi I-1, in contrast to the other Hi variants, contains a novel sequence motif that is not known from other proteins but is also present in single Hi variants of other, evolutionarily distant organisms. An N-terminal peptide of Hi I-1 that includes this motif also differs from the homologous region in another Hi variant of C. thummi by competing with the drug Hoechst 33258 for binding to the minor groove of the DNA double helix. Interaction of the subtype-specific motif with linker DNA may play a role in intranuclear targeting of Hi I-1 or in establishing a condensed type of chromatin, or in both. MATERIALS AND METHODSClone Isolation and DNA Sequencing. Genomic clones containing histone gene sequences were identified in a C. th. thummi library (13) 2481The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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Epigenetics in Algae

Tyler

2023

Epigenetics in Aquaculture

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Two histone H1-encoding genes of the green alga Volvox carteri with features intermediate between plant and animal genes

Cited by 20 publications

References 43 publications

Molecular cloning, sequence analysis and differential expression of an intron‐containing gene encoding tomato histone H1

Molecular cloning, sequence analysis and differential expression of an intron‐containing gene encoding tomato histone H1

Structural and functional differences between histone H1 sequence variants with differential intranuclear distribution.

Epigenetics in Algae

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