Two High-Throughput Techniques for Determining Wood Properties as Part of a Molecular Genetics Analysis of Hybrid Poplar and Loblolly Pine

Tuskan, Gerald A.; West, D.C.; Bradshaw, H. D.; Neale, David B.; Sewell, Mitchell M.; Wheeler, Nicholas C.; Megraw, Bob; Jech, K. S.; Wiselogel, A. E.; Evans, Robert J.; Elam, Carolyn C.; Davis, Mark F.; Dinus, Ron

doi:10.1385/abab:77:1-3:55

Cited by 59 publications

(12 citation statements)

References 14 publications

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“…Current wet chemical techniques for studying biomass composition are laborious and generally require harsh reagents such as acetyl bromide [11][12][13], sulfuric acid [14][15][16][17][18][19][20], trifluoroacetic acid [16,21,22], boron trifluoride etherate in thioacidolysis [23,24], and nitrobenzene [11,25]. Analytical techniques such as high-performance liquid chromatography (HPLC) [17,19,20,[26][27][28][29][30][31][32], gas chromatography(GC) or GC/mass spectrometry (GCMS) [33][34][35][36][37][38][39], pyrolysis (pyro) GCMS/molecular beam (MB) MS [40][41][42][43][44][45][46][47][48][49][50][51], thermogravimetric analysis [52][53]…”

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confidence: 99%

Assessment of Lignocellulosic Biomass Using Analytical Spectroscopy: an Evolution to High-Throughput Techniques

et al. 2013

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Lignocellulosic biomass has been proposed as an option for reducing global dependence on nonrenewable energy sources, such as oil. Selection and development of biomass feedstocks that efficiently yield the maximum fuel or biomaterial requires the availability of reliable methods for compositional and structural characterization of plant material. Many standard methods for biomass analysis are laborious and slow, and employ a variety of harsh reagents requiring some degree of remediation. The use of simpler and more rapid spectroscopic methods has proved invaluable in analyzing biomass. In the twenty-first century, researchers have employed techniques such as Raman, mid-infrared, and near-infrared spectroscopy for a wide range of applications in endeavors to further understand biofuel feedstocks. While many methods remain time consuming and expensive, a growing interest in high-throughput spectroscopic techniques has provided faster and larger scale feedstock screening for desirable traits. This review seeks to provide an overview of both high-throughput techniques and those requiring longer analysis times but still providing abundant qualitative and quantitative data. While applications of these instrumental methods have been researched for decades, more recent developments will be discussed here.

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Assessment of Lignocellulosic Biomass Using Analytical Spectroscopy: an Evolution to High-Throughput Techniques

et al. 2013

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“…Traditional wet chemistry techniques for the analysis of biomass require strong acids and time-consuming processes resulting in a method whereby only 20 samples per week per person can be analyzed [9]. Current advancements in analytical methods include infrared spectroscopy (Fourier transform infrared [FT-IR] and near-infrared [NIR]) and pyrolysis molecular beam mass spectroscopy (pyMBMS) [10][11][12][13]. Multivariate analyses are often used in conjunction with these methods.…”

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confidence: 99%

High-resolution Thermogravimetric Analysis For Rapid Characterization of Biomass Composition and Selection of Shrub Willow Varieties

Serapiglia

Cameron

Stipanovic

et al. 2007

Appl Biochem Biotechnol

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The cultivation of shrub willow (Salix spp.) bioenergy crops is being commercialized in North America, as it has been in Europe for many years. Considering the high genetic diversity and ease of hybridization, there is great potential for genetic improvement of shrub willow through traditional breeding. The State University of New York-College of Environmental Science and Forestry has an extensive breeding program for the genetic improvement of shrub willow for biomass production and for other environmental applications. Since 1998, breeding efforts have produced more than 200 families resulting in more than 5,000 progeny. The goal for this project was to utilize a rapid, low-cost method for the compositional analysis of willow biomass to aid in the selection of willow clones for improved conversion efficiency. A select group of willow clones was analyzed using high-resolution thermogravimetric analysis (HR-TGA), and significant differences in biomass composition were observed. Differences among and within families produced through controlled pollinations were observed, as well as differences by age at time of sampling. These results suggest that HR-TGA has a great promise as a tool for rapid biomass characterization.

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“…A commercially available molecular beam mass spectrometer (MBMS) designed specifically for biomass analysis was used for pyrolysis vapor analysis (Evans and Milne, 1987;Tuskan et al, 1999). Approximately 4 mg of air dried 20 mesh biomass was introduced into the quartz pyrolysis reactor via 80 uL deactivated stainless steel Eco-Cups provided with the autosampler.…”

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confidence: 99%

Pleiotropic and Epistatic Network-Based Discovery: Integrated Networks for Target Gene Discovery

Weighill

Jones

Shah

et al. 2018

Preprint

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Biological organisms are complex systems that are composed of functional networks of interacting molecules and macromolecules. Complex phenotypes are the result of orchestrated, hierarchical, heterogeneous collections of expressed genomic variants. However, the effects of these variants are the result of historic selective pressure and current environmental and epigenetic signals, and, as such, their co-occurrence can be seen as genome-wide correlations in a number of different manners. Biomass recalcitrance (i.e., the resistance of plants to degradation or deconstruction, which ultimately enables access to a plant's sugars) is a complex polygenic phenotype of high importance to biofuels initiatives. This study makes use of data derived from the re-sequenced genomes from over 800 different Populus trichocarpa genotypes in combination with metabolomic and pyMBMS data across this population, as well as co-expression and co-methylation networks in order to better understand the molecular interactions involved in recalcitrance, and identify target genes involved in lignin biosynthesis/degradation. A Lines Of Evidence (LOE) scoring system is developed to integrate the information in the different layers and quantify the number of lines of evidence linking genes to lignin-related lignin-phenotypes across the network layers. The resulting Genome Wide Association Study networks, integrated with Single Nucleotide Polymorphism (SNP) correlation, co-methylation and co-expression networks through the LOE scores are proving to be a powerful approach to determine the pleiotropic and epistatic relationships underlying cellular functions and, as such, the molecular basis for complex phenotypes, such as recalcitrance.

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Two High-Throughput Techniques for Determining Wood Properties as Part of a Molecular Genetics Analysis of Hybrid Poplar and Loblolly Pine

Cited by 59 publications

References 14 publications

Assessment of Lignocellulosic Biomass Using Analytical Spectroscopy: an Evolution to High-Throughput Techniques

Assessment of Lignocellulosic Biomass Using Analytical Spectroscopy: an Evolution to High-Throughput Techniques

High-resolution Thermogravimetric Analysis For Rapid Characterization of Biomass Composition and Selection of Shrub Willow Varieties

Pleiotropic and Epistatic Network-Based Discovery: Integrated Networks for Target Gene Discovery

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