Two Heteromeric Kinesin Complexes in Chemosensory Neurons and Sensory Cilia of<i>Caenorhabditis elegans</i>

Signor, Dawn; Wedaman, Karen P.; Rose, Lesilee S.; Scholey, Jonathan M.

doi:10.1091/mbc.10.2.345

Cited by 140 publications

(144 citation statements)

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“…Discovered in sea urchin eggs (23), KIF3 is present in a broad range of species, including Chlamydomonas (24,25), Caenorhabditis elegans (26), and zebrafish (10,27,28). Germline deletion of mouse KIF3a is embryonically lethal, and photoreceptor-specific dis-* This work was supported by National Institutes of Health Grants EY08123, ruption of KIF3A caused OS protein mistrafficking with rapid degeneration (29,30).…”

Section: Intraflagellar Transport (Ift) Is a Bidirectional Ciliary Trmentioning

confidence: 99%

Heterotrimeric Kinesin-2 (KIF3) Mediates Transition Zone and Axoneme Formation of Mouse Photoreceptors

Jiang

Wei

Ronquillo

et al. 2015

Journal of Biological Chemistry

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Background: Heterotrimeric kinesin-2 (KIF3) has been implicated in intraflagellar trafficking of photoreceptor membrane proteins by IFT. Results: KIF3 and IFT88 are required for transition zone and axoneme formation, but are dispensable for rhodopsin trafficking. Conclusion: Transmembrane proteins, including rhodopsin, traffic to the OS even when IFT is disabled. Significance: KIF3 builds and maintains the photoreceptor transition zone and axoneme that are essential for photoreceptor integrity and vision.

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Section: Intraflagellar Transport (Ift) Is a Bidirectional Ciliary Trmentioning

confidence: 99%

Heterotrimeric Kinesin-2 (KIF3) Mediates Transition Zone and Axoneme Formation of Mouse Photoreceptors

Jiang

Wei

Ronquillo

et al. 2015

Journal of Biological Chemistry

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“…Recombinant p50 was expressed and purified in injection buffer (150 mM potassium aspartate, 10 mM potassium phosphate, 20 mM imidazole, pH 7.2) under nondenaturing conditions on Ni-nitrilotriacetic acid Superflow resin (Qiagen, Valencia, CA) using standard purification procedures (Signor et al, 1999). Column fractions were analyzed by SDS-PAGE, and peak fractions were dialyzed into injection buffer and concentrated for microinjection.…”

Section: Bacterial Expression and Purification Of Human P50 Dynamitinmentioning

confidence: 99%

Functional Coordination of Three Mitotic Motors inDrosophilaEmbryos

Sharp

Brown

Kwon

et al. 2000

MBoC

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217

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It is well established that multiple microtubule-based motors contribute to the formation and function of the mitotic spindle, but how the activities of these motors interrelate remains unclear.Here we visualize spindle formation in living Drosophila embryos to show that spindle pole movements are directed by a temporally coordinated balance of forces generated by three mitotic motors, cytoplasmic dynein, KLP61F, and Ncd. Specifically, our findings suggest that dynein acts to move the poles apart throughout mitosis and that this activity is augmented by KLP61F after the fenestration of the nuclear envelope, a process analogous to nuclear envelope breakdown, which occurs at the onset of prometaphase. Conversely, we find that Ncd generates forces that pull the poles together between interphase and metaphase, antagonizing the activity of both dynein and KLP61F and serving as a brake for spindle assembly. During anaphase, however, Ncd appears to have no effect on spindle pole movements, suggesting that its activity is downregulated at this time, allowing dynein and KLP61F to drive spindle elongation during anaphase B. INTRODUCTIONThe segregation of chromosomes during mitosis depends on the action of a self-organizing, bipolar machine called the mitotic spindle. It is now established that the formation and function of the mitotic spindle requires numerous microtubule (MT)-based motor proteins (Hoyt and Geiser, 1996;Vale and Fletterick, 1997). Although the identities of many of these mitotic motors are becoming clear, their specific functional interrelationships have been extremely difficult to ascertain.Among all mitotic movements, the positioning of spindle poles during the assembly and elongation of the bipolar mitotic spindle may require the greatest degree of cooperation between different motors. This process is particularly complex because it occurs in a pathway consisting of several, temporally distinct stages, during which the organization of spindle microtubules and the general environment of the cell change dramatically (McIntosh and McDonald, 1989). The members of at least three families of MT motors are thought to play important roles in this pathway. These are the bipolar kinesins, the C-terminal kinesins, and cytoplasmic dynein.The bipolar (or BimC) kinesins (Vale and Fletterick, 1997) comprise a family of plus-end-directed motors, which have a bipolar morphology with motor domains at both ends of a central rod (Cole et al., 1994; Kashina et al., 1996a,b; Gordon and Roof, 2000). Functionally, these motors are thought to play a role in either the assembly or maintenance of spindle bipolarity, because their inhibition results in the formation of monopolar mitotic spindles (Enos and Morris, 1990;Hagan and Yanagida, 1990;Roof et al., 1991;Hoyt et al., 1992;Sawin et al., 1992;Heck et al., 1993;Blangy et al., 1995;Sharp et al., 1999b). Support for a role for bipolar kinesins in spindle maintenance but not assembly comes from the recent findings that inhibiting the Drosophila bipolar kinesin KLP61F does not pr...

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“…No movement was observed in the neuronal cilia, but movement of these marker proteins occurred normally in the dendrites [Signor et al, 1999a]. Signor et al [1999b] identified and characterized two kinesins that localize to the cilia of worm chemosensory neurons: the heterotrimeric kinesin II and the dimeric kinesin OSM-3. J. Scholey reported at the meeting that these two forms of kinesin appear to drive distinct IFT pathways in the worm.…”

Section: Osmotic Sensingmentioning

confidence: 99%

A healthy understanding of intraflagellar transport

Sloboda

2002

Cell Motil. Cytoskeleton

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A microtubule-dependent motility process called intraflagellar transport (IFT) occurs beneath the plasma membrane of cilia and flagella. IFT was first observed in Chlamydomonas, and orthologs of some of the polypeptides involved in IFT have recently been identified in other organisms, including C. elegans and the mouse. In addition to a role in the assembly and maintenance of cilia and flagella, evidence is reviewed here that indicates defects in the process of IFT may be related to problems with human health. Moreover, recent data suggest the possibility of two new roles for IFT in cell function. The first is in transcriptional control of the genes encoding ciliary and flagellar proteins. IFT could provide a mechanism whereby the cell senses the presence or absence of its cilia or flagella and responds by turning on gene transcription resulting in replacement of the missing organelle. The second role is in signal transduction, whereby cilia act as sensors of the external cellular environment and transduce information about the surroundings into intracellular signals that are sent via IFT to the cell body, thus inducing an appropriate cellular response to the environment. Cell Motil.

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Two Heteromeric Kinesin Complexes in Chemosensory Neurons and Sensory Cilia ofCaenorhabditis elegans

Cited by 140 publications

References 58 publications

Heterotrimeric Kinesin-2 (KIF3) Mediates Transition Zone and Axoneme Formation of Mouse Photoreceptors

Heterotrimeric Kinesin-2 (KIF3) Mediates Transition Zone and Axoneme Formation of Mouse Photoreceptors

Functional Coordination of Three Mitotic Motors inDrosophilaEmbryos

A healthy understanding of intraflagellar transport

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