Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression.

Özcan, Sabire; Dover, Jim; Rosenwald, Anne G.; Wölfl, Stefan; Johnston, Mark

doi:10.1073/pnas.93.22.12428

Cited by 375 publications

(443 citation statements)

References 26 publications

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“…It has recently been shown that sugar transporters can be involved in sugar sensing in yeast (Ozcan et al, 1996). Although evidence of such a role for VfSTPI and VfSUCI is missing, we have shown that VfSTPI and VfSUTI are highly homologous to other plant transporters responsible for sugar uptake.…”

Section: Transfer Cell Formation and Vfsutl Expression Are Lnduced Bymentioning

confidence: 65%

A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seeds.

et al. 1997

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To analyze sugar transport processes during seed development of fava bean, we cloned cDNAs encoding one sucrose and one hexose transporter, designated VfSUTl and VfSTPl, respectively. Sugar uptake activity was confirmed after heterologous expression in yeast. Gene expression was studied in relation to seed development. Transcripts were detected in both vegetative and seed tissues. In the embryo, VfSUTl and VfSTP 7 mRNAs were detected only in epidermal cells, but in a different temporal and spatial pattern. VfSTPl mRNA accumulates during the midcotyledon stage in epidermal cells covering the mitotically active parenchyma, whereas the VfSUTl transcript was specific to outer epidermal cells showing transfer cell morphology and covering the storage parenchyma. Transfer cells developed at the contact area of the cotyledonary epidermis and the seed coat, starting first at the early cotyledon stage and subsequently spreading to the abaxial region at the late cotyledon stage. Feeding high concentrations of sugars suppressed both VfSUT7 expression and transfer cell differentiation in vitro, suggesting a control by carbohydrate availability.

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Section: Transfer Cell Formation and Vfsutl Expression Are Lnduced Bymentioning

confidence: 65%

A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seeds.

et al. 1997

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“…In anaerobic glucose-limited chemostats, while glucose is efficiently transported and a sustained glycolytic flux is active inside the cell, the extracellular residual glucose concentration is low (,0.3 mM) compared to the K m of the high-affinity sensing systems (Ozcan et al, 1996;Rolland et al, 2000). This suggests that high levels of extracellular glucose are not required for stimulating Sfp1 activity.…”

Section: Discussion Continuous Cultures: Subsiding Growth Rate Effectsmentioning

confidence: 99%

Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study

et al. 2008

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Saccharomyces cerevisiae SFP1 promotes transcription of a large cluster of genes involved in ribosome biogenesis. During growth in shake flasks, a mutant deleted for SFP1 shows a small size phenotype and a reduced growth rate. We characterized the behaviour of an sfp1D mutant compared to an isogenic reference strain growing in chemostat cultures at the same specific growth rate. By studying glucose (anaerobic)-and ethanol (aerobic)-limited cultures we focused specifically on nutrient-dependent effects. Major differences in the genome-wide transcriptional profiles were observed during glucose-limited growth. In particular, Sfp1 appeared to be involved in the control of ribosome biogenesis but not of ribosomal protein gene expression. Flow cytometric analyses revealed size defects for the mutant under both growth conditions. Our results suggest that Sfp1 plays a role in transcriptional and cell size control, operating at two different levels of the regulatory network linking growth, metabolism and cell size.

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“…For example the Escherichia coli narX and narQ genes encode transmembrane histidine kinases which act as external NO 3 − receptors and, in the presence of external NO 3 − or NO 2 − , activate transcription factors encoded by the narL and narP genes respectively (Merrick and Edwards, 1995). In budding yeast (Saccharomyces cerevisiae), transmembrane sensors for glucose, amino acids and NH 4 + have been identified and found to be homologous to membrane proteins that act as transporters for the respective nutrients (Klasson et al, 1999;Lorenz and Heitman, 1998;Ozcan et al, 1996).…”

Section: Localized Responsesmentioning

confidence: 99%

The nutritional control of root development

Forde

Lorenzo

2002

Interactions in the Root Environment: An Integrated Approach

221

254

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Root development is remarkably sensitive to variations in the supply and distribution of inorganic nutrients in the soil. Here we review examples of the ways in which nutrients such as N, P, K and Fe can affect developmental processes such as root branching, root hair production, root diameter, root growth angle, nodulation and proteoid root formation. The nutrient supply can affect root development either directly, as a result of changes in the external concentration of the nutrient, or indirectly through changes in the internal nutrient status of the plant. The direct pathway results in developmental responses that are localized to the part of the root exposed to the nutrient supply; the indirect pathway produces systemic responses and seems to depend on long-distance signals arising in the shoot. We propose the term 'trophomorphogenesis' to describe the changes in plant morphology that arise from variations in the availability or distribution of nutrients in the environment. We discuss what is currently known about the mechanisms of external and internal nutrient sensing, the possible nature of the long-distance signals and the role of hormones in the trophomorphogenic response.Abbreviations: ABA, abscisic acid; NR, nitrate reductase; P i , inorganic phosphate

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Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression.

Cited by 375 publications

References 26 publications

A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seeds.

A role for sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in fava bean seeds.

Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study

The nutritional control of root development

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