Two genetic stocks of Steindachneridion melanodermatum living in sympatry in nature and genetic variability of wild parents and F1 generation

Matoso, Daniele Aparecida; Silva, M. da; Cortinhas, M.C.S.; Cestari, Marta Margarete; Almeida, Mara Cristina de; Vicari, Marcelo Ricardo; Artoni, Roberto Ferreira

doi:10.4238/2011.october.25.8

Cited by 3 publications

(2 citation statements)

References 23 publications

(26 reference statements)

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“…This is a migratory species, which is subject to potential extinction (SUZUKI et al, 2003), and due to dams on the Iguaçu River, its current distribution is restricted to the stretches between hydroelectric plants ( GARAVELLO, 2005). These facts and the lack of information regarding Steindachneridion reproductive biology (HONJI et al, 2012) suggest advanced research on their genetic variability (MATOSO et al, 2011) and artificial reproduction. Moreover, due to their growth characteristics and meat quality, research has been conducted to assess the nutritional aspects (LEWANDOWSKI et al, 2013) and its potential for rearing in net cages (FEIDEN et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Marcos

Neumann

Toledo

et al. 2015

SCA

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This study describes the seminal and spermatic characteristics of fresh semen of Steindachneridion melanodermatum and investigates the effects of dilution, temperature, and storage period on its spermatic parameters. Sperm samples were collected from nine hormonally-induced males. The following parameters in fresh sperm were analyzed: seminal plasma osmolality (OSM), seminal pH, sperm motility (MOT), sperm velocity (SV) (including sperm curvilinear velocity (VCL), sperm straight-line velocity (VSL), and sperm average path velocity (VAP)), total time of sperm motility (TEMP), sperm concentration (CONC), and index of sperm normality (NORM). Sperm samples from each male were diluted in a solution containing 5% fructose and 5% powdered milk, and stored at 10°C and 25°C. The same was carried out for sperm samples not subjected to dilution. From these samples, MOT, VCL, VSL, VAP, SV, and TEMP were measured after 0 h, 5 h, 9 h, 18 h, 27 h, 36 h, 45 h, and 54 h. Males released 11.74 ± 5.38 mL of sperm, with an osmolality of 258.78 ± 29.36 mOsm.kg -1 and pH of 7.11 ± 0.31. The sperm presented a MOT of 99.86 ± 0.31% at a concentration of 1.03 × 10 10 ± 3.65 × 10 9 spermatozoa. The results indicate that sperm motility, sperm velocity, and total time of sperm activation were affected by dilution, storage temperature, and storage period (p < 0.05). Procedures for semen storage should be performed with undiluted sperm cooled at 10°C, or kept undiluted at 25°C for up to 27 h. Key words: CASA, endemic, sperm characterization, sperm motility, sperm storage, sperm velocity ResumoO objetivo deste estudo foi descrever os parâmetros seminais e espermáticos no sêmen fresco do Steindachneridion melanodermatum e os efeitos da diluição, da temperatura e do tempo de estocagem do sêmen sobre os parâmetros espermáticos. Foi coletado o sêmen de nove machos, induzidos hormonalmente. No sêmen fresco foram avaliados os parâmetros: osmolaridade do plasma seminal (OSM), pH seminal (pH), motilidade (MOT) e velocidades espermáticas (VCL, VLR, VMD, VE),

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Section: Introductionmentioning

confidence: 99%

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Marcos

Neumann

Toledo

et al. 2015

SCA

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“…Although it is a relevant fishing resource, useful molecular markers such as microsatellites, extensively employed in fish genetic studies, are still not developed for any Steindachneridion species, and the knowledge about genetic characteristics of the genus is incipient. RAPD markers indicated low genetic diversity for S. scriptum from the UUR, as reported by Ramella et al (2006) , as well as for S. melanodermatum from the Iguaçu River Basin ( Matoso et al, 2011 ). Low genetic diversity may indicate recent or historic reduction of this diversity; however, some endangered populations may have a historic maintenance of small effective population sizes ( Matocq and Villablanca, 2001 ).…”

Section: Discussionmentioning

confidence: 67%

Genetic Variation of the Endangered Neotropical Catfish Steindachneridion scriptum (Siluriformes: Pimelodidae)

2018

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Steindachneridion scriptum is an important species as a resource for fisheries and aquaculture; it is currently threatened and has a reduced occurrence in South America. The damming of rivers, overfishing, and contamination of freshwater environments are the main impacts on the maintenance of this species. We accessed the genetic diversity and structure of S. scriptum using the DNA barcode and control region (D-loop) sequences of 43 individuals from the Upper Uruguay River Basin (UUR) and 10 sequences from the Upper Paraná River Basin (UPR), which were obtained from GenBank. S. scriptum from the UUR and the UPR were assigned in two distinct molecular operational taxonomic units (MOTUs) with higher inter-specific K2P distance than the optimum threshold (OT = 0.0079). The COI Intra-MOTU distances of S. scriptum specimens from the UUR ranged from 0.0000 to 0.0100. The control region indicated a high number of haplotypes and low nucleotide diversity, compatible with a new population in recent expansion process. Genetic structure was observed, with high differentiation between UUR and UPR basins, identified by BAPS, haplotype network, AMOVA (FST = 0.78, p < 0.05) and Mantel test. S. scriptum from the UUR showed a slight differentiation (FST = 0.068, p < 0.05), but not isolation-by-distance. Negative values of Tajima’s D and Fu’s Fs suggest recent demographic oscillations. The Bayesian skyline plot analysis indicated possible population expansion from beginning 2,500 years ago and a recent reduction in the population size. Low nucleotide diversity, spatial population structure, and the reduction of effective population size should be considered for the planning of strategies aimed at the conservation and rehabilitation of this important fisheries resource.

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Genetic Diversity of the Surubim-Do-Iguaçu, a Giant Catfish Species Threatened with Extinction: Recommendations for Species Conservation

et al. 2021

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Steindachneridion melanodermatum is the largest catfish of the Lower Iguaçu River and is endangered due to the habitat fragmentation caused by dams. Currently, the wild population’s last refuge is restricted to an area of 190 km. This study presents the first analysis of its genetic diversity and population structure, using microsatellite loci and mtDNA. The population has an adequate level of genetic diversity, but signs of a recent bottleneck were observed. The Baixo Iguaçu Hydroelectric Power Plant has recently fragmented the population and threatened it with extinction in a reduced area of nearly 30 km. Based on our results, we strongly advise against the stocking of breeding specimens below the Salto Caxias HPP to not compromise the integrity of the native gene pools at the receptor sites. In addition, we recommend manual fish transposition, trap-and-haul, to maintain the genetic connectivity of individuals upstream and downstream of the dam as a conservation strategy. Furthermore, studies on behavior and swimming capacities, and suitable fishways for this species must be developed. We strongly recommend that the Lower Iguaçu River and its tributaries be protected and preserved as free from additional barriers to prevent future habitat disruption for the benefit of S. melanodermatum and several other endemic and endangered species.

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Two genetic stocks of Steindachneridion melanodermatum living in sympatry in nature and genetic variability of wild parents and F1 generation

Cited by 3 publications

References 23 publications

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Seminal and spermatic characteristics of fresh semen and the effects of sperm cooling in Steindachneridion melanodermatum (Garavello, 2005)

Genetic Variation of the Endangered Neotropical Catfish Steindachneridion scriptum (Siluriformes: Pimelodidae)

Genetic Diversity of the Surubim-Do-Iguaçu, a Giant Catfish Species Threatened with Extinction: Recommendations for Species Conservation

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