Two gap junction channel (innexin) genes of the Bombyx mori and their expression

Functional Interactions between Polydnavirus and Host Cellular Innexins

“…Gap junctions are widely distributed in insect tissues (29,51), and CsIV vnx genes are transcribed in several tissues of infected hosts (54). In principle, therefore, parasitic infection could result in altered intercellular communication in various physiological systems.…”

“…All are transcribed in multiple tissues of infected caterpillars, and VnxQ2 forms junctional plaques at appositional membranes of infected cells (54). Innexin2 (Inx2), one of the most highly conserved members of the insect innexin gene family, is expressed throughout lepi-dopteran larval stages in similar tissues (29,44). Therefore, there is scope for vinnexins to form de novo gap junctions and/or to interact with cellular innexins in infected host tissues.…”

Suppression subtractive hybridization (SSH) for isolation and characterization of genes related to testicular development in the giant tiger shrimp Penaeus monodon

“…In Bombyx mori, northern blotting and in situ hybridization revealed that Bm-Inx2 was expressed across all developmental stages and in various tissues, with high expression observed in the nervous system during embryogenesis. In contrast, Bm-Inx4 was transiently expressed at the germ-band formation stage of embryogenesis, and was specifically expressed in ovaries and testes during the larval and pupal stages (15).…”

Molecular Characterization, Localization, and Distribution of Innexins in the Silkworm, Bombyx mori

“…For each reaction, SYBR Ex Taq TM premix (Takara) was mixed with template cDNA and 5 pmol primers (forward 5 0 -GAA CCG GAA TCT TCT TTT GAG TAT-3 0 and reverse 5 0 -ACG GAG CAT GGG AAC CTA GA-3 0 ). The level of expression was compared with that of the BmINX2 and BmINX4 reported previously [7]. Real-time PCR was performed in triplicate for each gene with an ABI Prism 7300 Sequence Detector.…”

“…As a result of a search for innexin sequences registered in the silkworm genome research program (SGP, http://sgp.dna.affrc.go.jp/), we have newly identified four silkworm innexins, BmINX1, BmINX3, and two shaking-B, vital and lethal, in addition to the BmINX2 and BmINX4 previously reported [7]. Here, we report the cloning and expression analysis of a novel silkworm innexin3 and demonstrated that the subcellular localization of the BmInx4 is different from the other innexins.…”