Two fungal endophytes reduce the severity of pitch canker disease in Pinus radiata seedlings

Martínez‐Álvarez, Pablo; Fernández-González, Raúl Arcadio; Sanz-Ros, Antonio V.; Pando, Valentín; Diez, Julio J.

doi:10.1016/j.biocontrol.2015.11.011

Cited by 44 publications

(45 citation statements)

References 49 publications

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“…was close to being negative, according to analysis parameters. In consequence, a larger sample size supported with a confrontation assay could provide more data about the potential antagonistic effects among these fungal taxa [87].…”

Section: Discussionmentioning

confidence: 99%

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Muñoz‐Adalia

Sanz-Ros

Flores-Pacheco

et al. 2017

Forests

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Bark beetles (Coleoptera, Scolytinae) carry a diverse filamentous fungal community sometimes acting as vectors or carriers of phytopathogens. In this study, mycobiota carried by two Tomicus species (Tomicus piniperda and Tomicus destruens) were investigated through (i) morphological and molecular identification of taxa; (ii) taxonomic richness, diversity, evenness, dominance and phoresy indices; (iii) ecological network analysis and (iv) statistical co-occurrence analysis. The studied mycobiota were formed by eleven taxa and showed a moderate fungal diversity with low evenness. The fungus Sydowia polyspora was significantly abundant and dominated the community. All the fungal taxa were randomly associated. Both insect species (T. piniperda and T. destruens) were collected from plantations of Pinus radiata infected by Fusarium circinatum. The ecological factors that could drive community ecology and phoretic links between fungi and bark beetles are discussed.

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Section: Discussionmentioning

confidence: 99%

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Muñoz‐Adalia

Sanz-Ros

Flores-Pacheco

et al. 2017

Forests

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“…Inoculations were carried out by the stem inoculation technique (Martínez-Álvarez et al, 2016). U-shaped wounds were cut into the bark of the seedlings, at 5-7 cm above ground, with a sterile scalpel (Correll et al, 1991).…”

Section: In Vivo Experiments: Pathogenicity Testsmentioning

confidence: 99%

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

et al. 2018

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Aim of the study: To assess the impact on two mycoviruses recently described in F. circinatum mitovirus 1 and 2-2 (FcMV1 and FcMV2-2) on i) mycelial growth, ii) spore germination and iii) relative necrosis.Material and methods: Fourteen monosporic strains of F. circinatum (one of each pair infected with mycoviruses and the other without them) of the pathogen with and without viruses were selected for the assay. The statistical analysis, were a linear mixed model of analysis of variance considering one between-subjects factor (isolate) and one within-subjects factor with four levels (1=without viruses, 2=only virus FcMV1, 3=only virus FcMV2-2 and 4=both viruses).Main results: Colony growth rates of F. circinatum isolates were significantly reduced in presence of mycoviruses (p=0.002). The spore germination was also reduced in the F. circinatum isolates containing mycovirus as compared to mycovirus-free isolates (p<0.001). No significant differences in lesion lengths caused by F. circinatum were found in relation to the presence/absence of mycovirus (p<0.61).Research highlights: Reduction of the percentage of spore germination in the isolates of F. circinatum with mycovirus infections, as compared to free isolates, provides indications of reduction of metabolic activity and plant physiology are discussed. The lack of significant differences found in the length of the lesions caused by F. circinatum with respect to the presence/absence of mycovirus, indicates that further studies with a larger number of variables are required Additional keywords: pine pitch canker; hypovirulence; biological control; forest pathology.

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“…La evaluación de la acción antagónica de cada especie se determinó por medio de la estimación del área de crecimiento micelial ocupada por cada especie. Se usaron cuatro indicadores para medir el efecto antagonista de los hongos endófitos sobre el patógeno (Martínez-Álvarez, 2016 …”

Section: Efecto Protector De Hongos Antagonistas Contra La Infección unclassified

“…De acuerdo a los trabajos publicados por Martínez-Álvarez et al, (2012Martínez-Álvarez et al, ( , 2016 las especies de Trichoderma spp., frente a F. circinatum. Demostró un elevado efecto de control inhibitorio por producción de micotoxinas de carácter antifúngicas combinadas con su capacidad de micoparasitar al huésped (Rolando et al, 2013).…”

Section: Análisis Estadísticounclassified

“…Particularmente, el uso de hongos endófitos, (Martínez-Álvarez, et al, 2016), tiene un futuro prometedor tanto por los resultados que proporciona (Bailey and Gilligan, 1997) como por la inocuidad en el ecosistema en que se emplee. Hasta el momento el control biológico ha sido empleado con éxito en el control de varias patologías vegetales (Trestić et al, 2001;Kwon et al, 2009;Romeralo, et al, 2013;Martínez-Álvarez, 2015).…”

Section: Introductionunclassified

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Antagonismo in vitro de hongos endófitos para su uso en el biocontrol de enfermedades forestales

Flores-Pacheco

2017

Rev. Científica FAREM-Estelí

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RESUMENLos hongos endófitos, son aquellos que colonizan los tejidos vivos internos de las plantas sin causar efecto significativo en el hospedante. Estos hongos, al ejercer en ocasiones, antagonismo frente a patógenos fúngicos a la planta hospedante, pueden ser una solución para el control de muchas de las enfermedades forestales causadas por otros hongos. Este campo ha sido poco evaluado en pruebas in vitro y en pruebas en vivo. Se utilizaron cinco hongos endófitos y seis hongos patógenos que se enfrentaron en medio de cultivo generalista Papa + Dextrosa +Agar (PDA) en condiciones de oscuridad a 25º C por siete días. Durante este periodo se midieron los ejes centrales y laterales de la colonia. Con estos datos se obtuvo el coeficiente de forma del hongo y se calculó el crecimiento directo, el coeficiente de forma del hongo patógeno frente al endófito respectivo, el porcentaje de inhibición del crecimiento radial y el porcentaje de inhibición de la zona de crecimiento. Se establecieron placas únicamente con el hongo patógeno a manera de grupo control. Los datos se analizaron con un Análisis de Varianza y para los casos estadísticamente significativos se aplicó la prueba de Diferencia Mínima Significativa. Los resultados muestran que los endófitos que tienen una mayor inhibición frente a los hongos patógenos. De esta manera se demuestra un efecto controlador de al menos un endófito sobre uno o más patógenos. Esto sugiere su posible utilidad como Agente de Control Biológico (ACB) ante enfermedades forestales. La reducción del crecimiento de los hongos patógenos ante la presencia de los endófitos, puede deberse a los mecanismos de acción de éstos (competencia y secreción de micotoxinas) frente al patógeno. Se deben realizar ensayos in vivo donde los endófitos se inoculen de acuerdo con su ecología y con un tiempo adecuado para la colonización de la planta.Palabras claves: control biológico, hongos patógenos, sanidad vegetal, antagonismo in vitro.

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Two fungal endophytes reduce the severity of pitch canker disease in Pinus radiata seedlings

Cited by 44 publications

References 49 publications

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Sydowia polyspora Dominates Fungal Communities Carried by Two Tomicus Species in Pine Plantations Threatened by Fusarium circinatum

Short communication: Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum

Antagonismo in vitro de hongos endófitos para su uso en el biocontrol de enfermedades forestales

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