Two fatty acid synthase genes from the integument contribute to cuticular hydrocarbon biosynthesis and cuticle permeability in <scp><i>Locusta migratoria</i></scp>

Yang, Yang; Zhao, Xiaoming; Niu, Niu; Zhao, Yiyan; Liu, Weimin; Moussian, Bernard; Zhang, Jianzhen

doi:10.1111/imb.12665

Cited by 21 publications

(23 citation statements)

References 35 publications

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“…In the model organism C. elegans , silencing FASN-1 at the larval stage ceases normal development and juvenile nematodes are not able to reach adulthood successfully [ 31 ]. In a recent study, Yang et al [ 32 ] reported that the injection of dsLmFAS1 and dsLmFAS3 caused about 80% mortality in Locusta migratoria . Therefore, FAS may be essential for insect development.…”

Section: Discussionmentioning

confidence: 99%

The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

Song

Liu

et al. 2022

IJMS

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Fatty acid synthase (FAS) is a key enzyme in the lipid synthesis pathway, however, its roles in insects remain largely unknown. Here, we firstly identified two FAS genes from the transcriptome dataset of the general cutworm Spodoptera litura, which is a destructive insect pest of many crops. Both SlFAS1 and SlFAS2 were highly expressed in third instar larvae and in their fat bodies. Then, we successfully silenced SlFAS1 in third instar larvae and the content of α-linolenic acid and triglyceride was significantly decreased. Besides that, the effect of FAS on the metamorphic development in S. litura was evaluated. The results indicate that after silencing SlFAS1, the survival rates of S. litura larvae decreased significantly compared to the control groups. Silencing SlFAS1 in fifth instar larvae resulted in more malformed pupae and adults, and the emergence rates were significantly reduced. Furthermore, the ecdysone content in the haemolymph of fifth instar larvae was significantly decreased after silencing SlFAS1. In addition, knocking down SlFAS1 significantly alters the expression of other key genes in the lipogenesis pathway, implying that FAS has an impact on the lipogenesis pathway. The present study deepens the understanding of FAS in insects and provides novel potential targets for managing insect pests.

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Section: Discussionmentioning

confidence: 99%

The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

Song

Liu

et al. 2022

IJMS

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“…More information on the genes such as estimated evolutionary rates based on orthogroup and phyletic profiles can be found in Table S1 in the supplementary information as well as more genes with an impact on CHC profiles whose function could not be assigned to a particular step in the CHC biosynthesis pathway. the mb-CHCs detected in this species, while both in-and decreasing the amounts of different n-alkane compounds with no apparent pattern (Yang et al 2020). Two further putative FAS genes have been identified in the genome of the seaweed fly Coelopa frigida, though their direct impact on CHC variation has not been investigated as of yet (Berdan et al 2019).…”

Section: Fatty Acid Synthase (Fas)mentioning

confidence: 90%

Advances in deciphering the genetic basis of insect cuticular hydrocarbon biosynthesis and variation

2020

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Cuticular hydrocarbons (CHCs) have two fundamental functions in insects. They protect terrestrial insects against desiccation and serve as signaling molecules in a wide variety of chemical communication systems. It has been hypothesized that these pivotal dual traits for adaptation to both desiccation and signaling have contributed to the considerable evolutionary success of insects. CHCs have been extensively studied concerning their variation, behavioral impact, physiological properties, and chemical compositions. However, our understanding of the genetic underpinnings of CHC biosynthesis has remained limited and mostly biased towards one particular model organism (Drosophila). This rather narrow focus has hampered the establishment of a comprehensive view of CHC genetics across wider phylogenetic boundaries. This review attempts to integrate new insights and recent knowledge gained in the genetics of CHC biosynthesis, which is just beginning to incorporate work on more insect taxa beyond Drosophila. It is intended to provide a stepping stone towards a wider and more general understanding of the genetic mechanisms that gave rise to the astonishing diversity of CHC compounds across different insect taxa. Further research in this field is encouraged to aim at better discriminating conserved versus taxon-specific genetic elements underlying CHC variation. This will be instrumental in greatly expanding our knowledge of the origins and variation of genes governing the biosynthesis of these crucial phenotypic traits that have greatly impacted insect behavior, physiology, and evolution.

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“…Free fatty acids were determined from the ds GFP- and ds DcGSK3 -treated samples according to the protocol reported by Yang et al with minor modifications [ 55 ]. Briefly, 40 D. citri were added to a 1.5 mL centrifugation tube and 1 mL of extracting solution was added before homogenizing on ice.…”

Section: Methodsmentioning

confidence: 99%

Silencing of Glycogen Synthase Kinase 3 Significantly Inhibits Chitin and Fatty Acid Metabolism in Asian Citrus Psyllid, Diaphorina citri

Zhang

2022

IJMS

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Glycogen is a predominant carbohydrate reserve in various organisms, which provides energy for different life activities. Glycogen synthase kinase 3 (GSK3) is a central player that catalyzes glucose and converts it into glycogen. In this study, a GSK3 gene was identified from the D. citri genome database and named DcGSK3. A reverse transcription quantitative PCR (RT-qPCR) analysis showed that DcGSK3 was expressed at a high level in the head and egg. The silencing of DcGSK3 by RNA interference (RNAi) led to a loss-of-function phenotype. In addition, DcGSK3 knockdown decreased trehalase activity, glycogen, trehalose, glucose and free fatty acid content. Moreover, the expression levels of the genes associated with chitin and fatty acid synthesis were significantly downregulated after the silencing of DcGSK3. According to a comparative transcriptomics analysis, 991 differentially expressed genes (DEGs) were identified in dsDcGSK3 groups compared with dsGFP groups. A KEGG enrichment analysis suggested that these DEGs were primarily involved in carbon and fatty acid metabolism. The clustering analysis of DEGs further confirmed that chitin and fatty acid metabolism-related DEGs were upregulated at 24 h and were downregulated at 48 h. Our results suggest that DcGSK3 plays an important role in regulating the chitin and fatty acid metabolism of D. citri.

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Two fatty acid synthase genes from the integument contribute to cuticular hydrocarbon biosynthesis and cuticle permeability in Locusta migratoria

Cited by 21 publications

References 35 publications

The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

Advances in deciphering the genetic basis of insect cuticular hydrocarbon biosynthesis and variation

Silencing of Glycogen Synthase Kinase 3 Significantly Inhibits Chitin and Fatty Acid Metabolism in Asian Citrus Psyllid, Diaphorina citri

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