Two-enzyme lactose biosensor based on β-galactosidase and glucose oxidase deposited by AC-electrophoresis: Characteristics and performance for lactose determination in milk

Ammam, Malika; Fransaer, Jan

doi:10.1016/j.snb.2010.05.027

Cited by 66 publications

(50 citation statements)

References 24 publications

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“…The essential drawback of the majority of existing analytical methods for lactose determination in milk was that many of them could not detect low lactose concentration (below 0.5 g.kg −1 ) (Ammam and Fransaer 2010;Sasic and Ozaki 2001) and could only be used for conventional lactose-containing milk samples. An attempt to use a chemometric PLS approach for lactose calibration in the NIR region was unsuccessful due to strong absorbance of water (Sasic and Ozaki 2001).…”

Section: Quantitative Nmr Methods For Lactose Analysismentioning

confidence: 99%

“…The most frequently used technique for lactose determination in milk and dairy products is an enzymatic assay based on spectrophotometric measurement, which is also the German reference method (Amtliche Sammlung von Untersuchungsverfahren 2010). Other approaches include biosensors (Ammam and Fransaer 2010), colorimetry (Bakos et al 2002), near-infrared (NIR) spectroscopy (Kawasaki et al 2008;Woo et al 2002), Fourier transform infrared spectroscopy (Lefier et al 1996), or chromatographic techniques (Eadala et al 2009;Paredes et al 2006;Schuster-Wolff-Bühring et al 2011). Besides being comparably time-consuming, none of these methods appear to be appropriate for our purpose to provide a rapid screening of nutrition parameters.…”

Section: Introductionmentioning

confidence: 99%

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NMR spectroscopy as a screening tool to validate nutrition labeling of milk, lactose-free milk, and milk substitutes based on soy and grains

Monakhova

Kuballa

Leitz

et al. 2011

Dairy Science & Technol.

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Lactose-free milk and milk substitutes based on soy, oat, or rice are widely marketed to lactose-intolerant consumers. In this study, 400 MHz 1 H nuclear magnetic resonance (NMR) spectroscopy was used in the context of food surveillance to validate the "lactose-free" claims labeled on these beverages. Using soft independent modeling of class analogy (SIMCA) analysis, a qualitative classification according to the type of beverage (lactose-containing milk, lactose-free milk, oat, soy, and rice milk substitutes) was possible. Furthermore, quantitative data regarding nutrition labeling parameters were predicted from the same spectra using partial least squares (PLS) regression. The models obtained for carbohydrate, sugars, protein, fat, saturates, and energy (R 2 =0.89-0.97) were suitable for a screening analysis. Using nicotinamide as an internal standard, quantitative determination of lactose with a detection limit of 0.03 g.L -1 was also possible using direct integration of the signals (linear range, 0.05-50.0 g.L −1 , R>0.999). The relative standard deviations for the lactose-free milks were below 10%. NMR spectroscopy was judged to be suitable for the rapid routine analysis of milk and milk substitutes.

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Section: Quantitative Nmr Methods For Lactose Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NMR spectroscopy as a screening tool to validate nutrition labeling of milk, lactose-free milk, and milk substitutes based on soy and grains

Monakhova

Kuballa

Leitz

et al. 2011

Dairy Science & Technol.

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show abstract

“…Enzyme-based amperometric biosensors have been found to constitute versatile analytical devices with high selectivity that can be operated by unskilled personnel. Accordingly, they can be envisaged as serious competitors for conventional techniques, representing an attractive alternative for small industries [17][18][19].…”

Section: 2mentioning

confidence: 99%

Recent advances in analytical techniques for the determination of lactose

Jia¹,

Dong

2016

Int. J. Curr. Res. Chem. Pharm. Sci

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Lactose is disaccharide which is composed of glucose and galactose, and is the only saccharide synthesized by mammals and is enriched in mammalian milk. Lactose plays multiple roles in the health of neonates, by stimulating the growth of selected beneficial bacteria in the gut, and participating in the development and growth of newborns. Lactose concentrations may be indicative of abnormalities and be used for clinical diagnosis. Thus, the establishment of a simple and rapid method for the determination of lactose with high selectivity and sensitivity is of great significance to dairy industry and public health. In this article the studies of detection methods for lactose in recent years are reviewed.

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“…This situation has stimulated the interest in developing electrochemical sensor-based methodologies for milk analysis and many examples can be found in the literature [3][4][5][6][7]. Electrochemical biosensors are particularly interesting because they show high selectivity and can play a fundamental role improving the quality control of milk [8,9]. During the last years, a variety of nanostructured electrodes have been developed.…”

Section: Introductionmentioning

confidence: 99%

Multisensor System Based on Layer by Layer Films. Discrimination of Milks

Salvo-Comino¹,

García-Hernández²,

Garcı́a³

et al. 2018

Preprint

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Abstract:A nanostructured electrochemical bi-sensor system for analysis of milks has been developed using the Layer by Layer technique. The non-enzymatic sensor [CHI+IL/CuPc S ]2, is a layered material containing a negative film of the anionic sulfonated copper phthalocyanine (CuPc S ) acting as electrocatalytic material, and a cationic layer containing a mixture of an ionic liquid (IL) (1-butyl-3-methylimidazolium tetrafluoroborate) that enhances the conductivity and chitosan (CHI) that facilitates the enzyme immobilization. The biosensor ([CHI+IL/CuPc S ]2-GAO) results from the immobilization of galactose oxidase on the top of the LbL layers. FTIR, UV-vis and AFM have confirmed the proposed structure and cyclic voltammetry has demonstrated the amplification caused by the combination of materials in the film. Sensors have been combined to form an electronic tongue for milk analysis. Principal Component Analysis has revealed the ability of the sensor system to discriminate between milk samples with different lactose content. Using PLS-1 calibration models, correlations have been found between the voltammetric signals and chemical parameters measured by classical methods. PLS-1 models provide excellent correlations with lactose content. Additional information about other components such as fats, proteins and acidity can also be obtained. The method developed is simple and the short response time permits its use in assaying milk samples on-line.

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Two-enzyme lactose biosensor based on β-galactosidase and glucose oxidase deposited by AC-electrophoresis: Characteristics and performance for lactose determination in milk

Cited by 66 publications

References 24 publications

NMR spectroscopy as a screening tool to validate nutrition labeling of milk, lactose-free milk, and milk substitutes based on soy and grains

NMR spectroscopy as a screening tool to validate nutrition labeling of milk, lactose-free milk, and milk substitutes based on soy and grains

Recent advances in analytical techniques for the determination of lactose

Multisensor System Based on Layer by Layer Films. Discrimination of Milks

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