Two Drosophila Melanogaster Tropomyosin Genes: Structural and Functional Aspects

Karlik, C C; Fyrberg, E A

doi:10.1128/mcb.6.6.1965-1973.1986

Cited by 52 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The N-terminal region of TM is highly conserved in the 284-residue TMs found in muscle and nonmuscle cells, being identical in Drosophila and rabbit (1,13). Its integrity is critical for the function of striated TM.…”

mentioning

confidence: 99%

The Structure of the N-Terminus of Striated Muscle α-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies^,

et al. 1998

View full text Add to dashboard Cite

Tropomyosins (TMs) are highly conserved, coiled-coil, actin binding regulatory proteins found in most eukaryotic cells. The amino-terminal domain of 284-residue TMs is among the most conserved and functionally important regions. The first nine residues are proposed to bind to the carboxyl-terminal nine residues to form the "overlap" region between successive TMs, which bind along the actin filament. Here, the structure of the N-terminus of muscle alpha-TM, in a chimeric peptide, TMZip, has been solved using circular dichroism (CD) and two-dimensional proton nuclear magnetic resonance (2D 1H NMR) spectroscopy. Residues 1-14 of TMZip are the first 14 N-terminal residues of rabbit striated alpha-TM, and residues 15-32 of TMZip are the last 18 C-terminal residues of the yeast GCN4 transcription factor. CD measurements show that TMZip forms a two-stranded coiled-coil alpha-helix with an enthalpy of folding of -34 +/- 2 kcal/mol. In 2D1H NMR studies at 15 degrees C, pH 6.4, the peptide exhibits 123 sequential and medium range intrachain NOE cross peaks per chain, characteristic of alpha-helices extending from residue 1 to residue 29, together with 85 long-range NOE cross peaks arising from interchain interactions. The three-dimensional structure of TMZip has been determined using these data plus an additional 509 intrachain constraints per chain. The coiled-coil domain extends to the N-terminus. Amide hydrogen exchange studies, however, suggest that the TM region is less stable than the GCN4 region. The work reported here is the first atomic-resolution structure of any region of TM and it allows insight into the mechanism of the function of the highly conserved N-terminal domain.

show abstract

mentioning

confidence: 99%

The Structure of the N-Terminus of Striated Muscle α-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies^,

et al. 1998

View full text Add to dashboard Cite

show abstract

“…The higher expression of mlc2 (spots W87–89) in this Acc species is possibly related to the molecular motor myosin, responsible for defining both structural and mechanical properties of the insect flight muscle . Yet, the exclusive expression of tm1(spot W66) in the antennae of the Aml worker indicates its involvement in the stretch-activated indirect flight muscle because the antennae can be used as a tool to measure flight speed, perceive odors, and detect even the direction of the odor . ,, …”

Section: Discussionmentioning

confidence: 99%

“…105 Yet, the exclusive expression of tm1(spot W66) in the antennae of the Aml worker indicates its involvement in the stretch-activated indirect flight muscle because the antennae can be used as a tool to measure flight speed, perceive odors, and detect even the direction of the odor . 17, 106,107 HSPs support the transportation, folding, and rearrangement of other proteins as a molecular chaperone under normal physiological conditions. 108,109 Hence, the higher expressions of hsp90 (spot D2), hsp60 (spot D7) and glycoprotein (gp93, spot D1) in the antennae of the Aml drone compared to the Acc drone may indicate their great contribution to assist proper protein conformation and prevention of unwanted protein aggregation.…”

Section: Journal Of Proteome Researchmentioning

confidence: 99%

Western Honeybee Drones and Workers (Apis mellifera ligustica) Have Different Olfactory Mechanisms than Eastern Honeybees (Apis cerana cerana)

et al. 2012

View full text Add to dashboard Cite

The honeybees Apis mellifera ligustica (Aml) and Apis cerana cerana (Acc) are two different western and eastern bee species that evolved in distinct ecologies and developed specific antennal olfactory systems for their survival. Knowledge of how their antennal olfactory systems function in regards to the success of each respective bee species is scarce. We compared the antennal morphology and proteome between respective sexually mature drones and foraging workers of both species using a scanning electron microscope, two-dimensional electrophoresis, mass spectrometry, bioinformatics, and quantitative real-time polymerase chain reaction. Despite the general similarities in antennal morphology of the drone and worker bees between the two species, a total of 106 and 100 proteins altered their expression in the drones' and the workers' antennae, respectively. This suggests that the differences in the olfactory function of each respective bee are supported by the change of their proteome. Of the 106 proteins that altered their expression in the drones, 72 (68%) and 34 (32%) were overexpressed in the drones of Aml and Acc, respectively. The antennae of the Aml drones were built up by the highly expressed proteins that were involved in carbohydrate metabolism and energy production, molecular transporters, antioxidation, and fatty acid metabolism in contrast to the Acc drones. This is believed to enhance the antennal olfactory functions of the Aml drones as compared to the Acc drones during their mating flight. Likewise, of the 100 proteins with expression changes between the worker bees of the two species, 67% were expressed in higher levels in the antennae of Aml worker contrasting to 33% in the Acc worker. The overall higher expressions of proteins related to carbohydrate metabolism and energy production, molecular transporters, and antioxidation in the Aml workers compared with the Acc workers indicate the Aml workers require more antennal proteins for their olfactory mechanisms to perform efficient foraging activities than do the Acc worker bees. These data decipher the mechanisms of the western and eastern drone and worker bees acting in response to their different olfactory system in their distinct ecosystem.

show abstract

“…As one of the main regulators of actin cytoskeleton remodeling, tsr is required for many different processes in the cell, including cell motility, cell polarity during migration, endocytosis, axon guidance, and cytokinesis . However, the higher level of expression of tm1 (spots 23 and 61) in the antennae of the workers implies its association with the stretch-activated indirect flight muscle (IFM) because the antennae can be used as an instrument to measure flight speed, detect odors, and detect even the direction of the odor. , …”

Section: Discussionmentioning

confidence: 99%

“…52 However, the higher level of expression of tm1 (spots 23 and 61) in the antennae of the workers implies its association with the stretch-activated indirect flight muscle (IFM) because the antennae can be used as an instrument to measure flight speed, detect odors, and detect even the direction of the odor. 53,54 Development proteins were expressed in the antennae of both the drones and the workers. blw (spot 16) and idgf4 (spot 45) were the major proteins in the drones and workers, respectively.…”

Section: Discussionmentioning

confidence: 99%

Antennal Proteome Comparison of Sexually Mature Drone and Forager Honeybees

et al. 2011

View full text Add to dashboard Cite

Honeybees have evolved an intricate system of chemical communication to regulate their complex social interactions. Specific proteins involved in odorant detection most likely supported this chemical communication. Odorant reception takes place mainly in the antennae within hairlike structures called olfactory sensilla. Antennal proteomes of sexually mature drone and forager worker bees (an age group of bees assigned to perform field tasks) were compared using two-dimensional electrophoresis, mass spectrometry, quantitative real-time polymerase chain reaction, and bioinformatics. Sixty-one differentially expressed proteins were identified in which 67% were highly upregulated in the drones' antennae whereas only 33% upregulated in the worker bees' antennae. The antennae of the worker bees strongly expressed carbohydrate and energy metabolism and molecular transporters signifying a strong demand for metabolic energy and odorant binding proteins for their foraging activities and other olfactory responses, while proteins related to fatty acid metabolism, antioxidation, and protein folding were strongly upregulated in the drones' antennae as an indication of the importance for the detection and degradation of sex pheromones during queen identification for mating. On the basis of both groups of altered antenna proteins, carbohydrate metabolism and energy production and molecular transporters comprised more than 80% of the functional enrichment analysis and 45% of the constructed biological interaction networks (BIN), respectively. This suggests these two protein families play crucial roles in the antennal olfactory function of sexually mature drone and forager worker bees. Several key node proteins in the BIN were validated at the transcript level. This first global proteomic comparative analysis of antennae reveals sex-biased protein expression in both bees, indicating that odorant response mechanisms are sex-specific because of natural selection for different olfactory functions. To the best of our knowledge, this result further provides extensive insight into the expression of the proteins in the antennae of drone and worker honeybees and adds vital information to the previous findings. It also provides a new angle for future detailed functional analysis of the antennae of the honeybee castes.

show abstract

Two Drosophila Melanogaster Tropomyosin Genes: Structural and Functional Aspects

Cited by 52 publications

References 31 publications

The Structure of the N-Terminus of Striated Muscle α-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies^,

The Structure of the N-Terminus of Striated Muscle α-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies^,

Western Honeybee Drones and Workers (Apis mellifera ligustica) Have Different Olfactory Mechanisms than Eastern Honeybees (Apis cerana cerana)

Antennal Proteome Comparison of Sexually Mature Drone and Forager Honeybees

Contact Info

Product

Resources

About

Two Drosophila Melanogaster Tropomyosin Genes: Structural and Functional Aspects

Cited by 52 publications

References 31 publications

The Structure of the N-Terminus of Striated Muscle α-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies,

The Structure of the N-Terminus of Striated Muscle α-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies,

Western Honeybee Drones and Workers (Apis mellifera ligustica) Have Different Olfactory Mechanisms than Eastern Honeybees (Apis cerana cerana)

Antennal Proteome Comparison of Sexually Mature Drone and Forager Honeybees

Contact Info

Product

Resources

About

The Structure of the N-Terminus of Striated Muscle α-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies^,

The Structure of the N-Terminus of Striated Muscle α-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies^,