Two distinct subtypes of human respiratory syncytial (RS) virus

Mufson, Maurice A.; Örvell, Claes; Rafnar, Björg; Norrby, Erling

doi:10.1016/0168-1702(85)90422-8

Cited by 2 publications

(2 citation statements)

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“…RSV is an enveloped virus belonging to the family Paramyxoviridae that has a single-stranded nonsegmented negative-sense RNA. There are two distinct groups (A and B) of RSV, which are distinguished by antigenic and genetic variations [5]. Respiratory infection of RSV may lead to bronchitis, bronchial pneumonia and severe pneumonia and can even be life-threatening.…”

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confidence: 99%

Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control

Zhang

et al. 2019

Arch Virol

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Human respiratory syncytial virus (RSV) is a common viral pathogen that causes lower respiratory tract infections in infants and children globally. In this study, we developed a duplex reverse transcription recombinase-aided amplification (duplex-rtRAA) assay containing an internal control in a single closed tube for the detection of human RSV. The internal control in the amplification effectively eliminated false-negative results and ensured the accuracy of the duplex-rtRAA system. We first developed and evaluated a universal singleplex-rtRAA assay for RSV. The sensitivity of this assay for RSV was determined as 4.4 copies per reaction, and the specificity was 100%. Next, a duplex-rtRAA assay with an internal control was established. The sensitivity of the duplex-rtRAA assay approached 5.0 copies per reaction, and no cross-reaction with other common respiratory viruses was observed. The two detection methods (singleplex-rtRAA and duplex-rtRAA) developed in this study were used to test 278 clinical specimens, and the results showed absolute consistency with RSV RT-qPCR analysis, demonstrating 100% diagnostic sensitivity and specificity. These data indicate that the duplex-rtRAA has great potential for the rapid detection of RSV with a high sensitivity.Acute respiratory tract infections (ARTIs) are one of the main causes of outpatient visits, hospitalization, and death, especially in children, the elderly and immunocompromised individuals [1-3]. The major pathogens causing ARTIs in infants and young children are viruses, the most frequently reported of which include respiratory syncytial virus (RSV),

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Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control

Zhang

et al. 2019

Arch Virol

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“…Strains are classified into two antigenic groups, A and B [2], representing separate genetic lineages whose divergence was calculated to have occurred 350 years ago [3]. The two groups co-circulate and are both responsible for epidemics, although group A usually dominates during epidemics [4].…”

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Early Evolution of Human Respiratory Syncytial Virus ON1 Strains: Analysis of the Diversity in the C-Terminal Hypervariable Region of Glycoprotein Gene within the First 3.5 Years since Their Detection

Ivancic-Jelecki

Forčić²,

Mlinarić‐Galinović³

et al. 2015

Intervirology

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Objective: Characterization of the phylogeny and diversity of human respiratory syncytial virus (HRSV) genotype ON1 that occurred during its early evolution (within the first 3.5 years since the detection of the first ON1 strains). ON1 strains have a 72-nucleotide-long in-frame duplication within the second hypervariable domain of the glycoprotein gene (HVR2). Methods: All available HVR2 sequences of strains belonging to the ON1 genotype published prior to June 20, 2014 were collected. Multiple sequence alignments, phylogeny, phylogeography, sequence clustering and putative protein analyses were performed. Results: The worldwide spread and diversification of ON1 strains are presented. Only in a minority of ON1 strains do the two replicas remain identical, and various ON1 strains possess common differences between the first and the second copy (segments A and B). Mutations of the progenitor sequence were more frequent in segment B, a higher overall diversity on the protein level and more putative glycosylation sites exist in segment B, and, unlike in segment A, positive selection acts on that protein region. Conclusions: The fast spread of the novel HRSV genotype ON1 has been accompanied by its rapid concurrent diversification. Differences in variability of the two replicas within HVR2 were detected, with C-terminal replica being more variable.

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Two distinct subtypes of human respiratory syncytial (RS) virus

Cited by 2 publications

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Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control

Development of a duplex reverse transcription recombinase-aided amplification assay for respiratory syncytial virus incorporating an internal control

Early Evolution of Human Respiratory Syncytial Virus ON1 Strains: Analysis of the Diversity in the C-Terminal Hypervariable Region of Glycoprotein Gene within the First 3.5 Years since Their Detection

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