Two distinct mtDNA lineages of the blue crab reveal large-scale population structure in its native Atlantic distribution

Rodrigues, Marcos Alaniz; Dumont, Luiz Felipe Cestari; Santos, Cleverson Ranniéri Meira dos; D’Incao, Fernando; Weiss, Steven; Froufe, Elsa

doi:10.1016/j.ecss.2017.08.004

Cited by 12 publications

(19 citation statements)

References 43 publications

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“…Expected heterozygosity for southern Brazil and the US is 0.59 and 0.84, respectively. This finding is congruent with the results of Rodrigues et al (2017), which examined COI DNA sequences and reported significant differences between the two regions; although as mentioned previously, this marker is problematic for genetic structure studies because of high levels of mitochondrial heteroplasmy in this species. Similarly, the RAD-sequencing study of Plough (2017) found that two individuals from Porto Alegre, Brazil, within the region sampled by Lacerda et al (2016), are highly differentiated from those in the two US localities he examined.…”

Section: Discussionsupporting

confidence: 92%

“…Nonetheless, the high estimated mtDNA diversity for C. sapidus may be influenced by the occurrence of high levels of heteroplasmy in this species (Williams, Feng & Place, 2017). MtDNA diversity estimates could also be inflated by misidentification; a highly divergent COI sequence found by Feng, Williams & Place (2017) could belong to C. similis or to a highly divergent lineage of C. sapidus so far restricted to Brazil (Rodrigues et al, 2017). Unfortunately, this cannot be verified because neither of these studies has made its sequence data publicly available.…”

Section: Discussionmentioning

confidence: 99%

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Population genetics and historical demographic inferences of the blue crabCallinectes sapidusin the US based on microsatellites

Macedo

Caballero

Mateos

et al. 2019

PeerJ

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The native range of the blue crab Callinectes sapidus spans Nova Scotia to northern Argentina. In the US, it constitutes a keystone species in estuarine habitats of the Atlantic coast and Gulf of Mexico (GOM), serving as both predator and prey to other species, and also has historically represented a multi-billion dollar fishery. Knowledge relevant to effective management and monitoring of this ecologically and economically important species, such as levels of population genetic differentiation and genetic diversity, is necessary. Although several population genetics studies have attempted to address these questions in one or more parts of its distribution, conflicting results and potential problems with the markers used, as well as other issues, have obscured our understanding on them. In this study, we examined large-scale genetic connectivity of the blue crab in the US, using 16 microsatellites, and genotyped individuals from Chesapeake Bay, in the US Atlantic, and from nine localities along the US GOM coast. Consistent with the high long-distance dispersal potential of this species, very low levels of genetic differentiation were detected for the blue crab among the ten US localities examined, suggesting it constitutes a large panmictic population within this region. Estimations of genetic diversity for the blue crab appear to be high in the US, and provide a baseline for monitoring temporal changes in this species. Demographic analyses indicate a recent range expansion of the US population, probably during the Holocene. In addition, capitalizing on published microsatellite data from southern Brazil, our analyses detected high genetic differentiation between localities in the US and Brazil. These results point to the need for examination of genetic diversity and differentiation along the area spanning the US to southern Brazil.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Population genetics and historical demographic inferences of the blue crabCallinectes sapidusin the US based on microsatellites

Macedo

Caballero

Mateos

et al. 2019

PeerJ

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“…Robles et al (2007) also found that C. maracaiboensis could not be reliably differentiated from C. bocourti, thus confirming previous suggestions by Schubart et al (2001) that it did not warrant separate species designation. With the Callinectes phylogeny better resolved, recent genetic 1 https://www.gbif.org/species/2225646 work in the genus has focused on examining phylogeographic patterns of C. sapidus across its range, confirming high withinregion gene flow (McMillen-Jackson and Bert, 2004;Feng et al, 2017) and prominent genetic divergences across the equator (Yednock and Neigel, 2014;Plough, 2017;Rodrigues et al, 2017;Macedo et al, 2019;Windsor et al, 2019). The increasing number of applied and evolutionary studies on the blue crab means that reliable and rapid species identification is paramount.…”

Section: Introductionmentioning

confidence: 99%

Rapid Genetic Identification of the Blue Crab Callinectes sapidus and Other Callinectes spp. Using Restriction Enzyme Digestion and High Resolution Melt (HRM) Assays

et al. 2020

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The blue crab Callinectes sapidus is one of the most widely studied marine crustaceans due to its high economic value and ecological significance. Despite extensive research on the blue crab in North America, many questions remain about the distribution and abundance of the species in the subtropics and tropics. In many places, C. sapidus is sympatric with morphologically similar Callinectes spp., which has implications for seafood mislabeling. To enable rapid identification of the species, we designed and tested two PCR-based assays targeting the 12S rRNA mitochondrial gene. The first assay discriminates C. sapidus from other Callinectes spp. via post-PCR restriction digestion (PCR-RFLP) and the second assay discriminates among multiple Callinectes spp. through High Resolution Melting (HRM) analysis and supervised machine learning analyses. A total of 58 DNA samples from five Callinectes spp. (validated via 12S gene sequencing) were used for assay testing. The PCR RFLP assay was 100% accurate identifying C. sapidus from other Callinectes spp. HRM analysis of amplicons showed good discrimination among species, with distinct clusters formed between species with higher sequence homology. Linear discriminant analysis (LDA) classification of HRM curves was quite successful given the small dataset available, producing ∼90-91% mean accuracy in classification over all species with 100-fold cross validation. Much of the error came from misclassifications between C. similis and C. danae, which are ∼99% similar in sequence for the amplicon; collapsing them into a single class increased overall classification success to 94%. Error also arose from C. bocourti classifications, which had a reference set containing only three samples. Classification accuracy of C. sapidus alone via HRM was 97.5%. Overall, these assays show great promise as rapid and inexpensive methods to identify Callinectes spp. and have application for both ecological research and seafood identification or labeling.

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“…Although many blue crab larvae return to their parent estuary, they can be transported among estuaries depending on oceanographic conditions (Epifanio et al 1989, Johnson & Hess 1990. Genetic studies suggest that high gene flow occurs among neighboring estuaries over ecological time scales (McMillen-Jackson et al 1994, McMillen-Jackson & Bert 2004, Rodrigues et al 2017. Settled juveniles migrate up-estuary to lower salinity waters, where they grow to maturity.…”

Section: Significancementioning

confidence: 99%

“…Lagos et al 2007, Rogers & Schindler 2008. As a focal example, blue crabs (Callinectes sapidus) on the Atlantic coast of the United States have nearly panmictic larval dispersal (McMillen-Jackson et al 1994, McMillen-Jackson & Bert 2004, Rodrigues et al 2017, and their dynamics are thought to be strongly influenced by spatially synchronized environmental drivers such as temperature and precipitation (e.g. Rome et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Ecological and environmental drivers of blue crab population dynamics across scales

Rogers¹

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Two distinct mtDNA lineages of the blue crab reveal large-scale population structure in its native Atlantic distribution

Cited by 12 publications

References 43 publications

Population genetics and historical demographic inferences of the blue crabCallinectes sapidusin the US based on microsatellites

Population genetics and historical demographic inferences of the blue crabCallinectes sapidusin the US based on microsatellites

Rapid Genetic Identification of the Blue Crab Callinectes sapidus and Other Callinectes spp. Using Restriction Enzyme Digestion and High Resolution Melt (HRM) Assays

Ecological and environmental drivers of blue crab population dynamics across scales

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