“…Moreover, detecting the highly informative and variable genome regions can be important for diagnostic genetic marker development and DNA barcoding [ 27 ]. In Fagopyrum, plastome regions rbcL , accD , trnK (UUU) , trnC (GCA) - rpoB , matK , trnH-psbA , trnL , psbE-psbL, and ndhA intron have been widely used to construct the phylogeny at the species level, which is often supplemented with nrDNA ITS and/or nuclear gene sequences ( FLO/LFY , AG ) [ 12 , 15 – 17 , 19 , 49 , 50 ]. In general, there is a greater variation in intergenic regions than in gene regions, as has also been observed in this study (Table S 6 ).…”