Two Distinct Genetic Elements Are Responsible for
            <i>erm</i>
            (TR)-Mediated Erythromycin Resistance in Tetracycline-Susceptible and Tetracycline-Resistant Strains of Streptococcus pyogenes

Brenciani, Andrea; Tiberi, Erika; Bacciaglia, Alessandro; Petrelli, Dezemona; Varaldo, Pietro E.; Giovanetti, Eleonora

doi:10.1128/aac.01378-10

Cited by 31 publications

(31 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S2 in the supplemental material). BLAST comparisons identified high homology (97% at the nucleotide level) between this mobile genetic element and group A Streptococcus integrative and conjugative element Sp1108 (ICESp1108) (36). In contrast, in ST1 strains, gene ermB is carried by Tn917 integrated into an ICE also carrying gene tetM (31), although some ST1 isolates do contain the ermTR gene.…”

Section: Resultsmentioning

confidence: 99%

Emergence of Serotype IV Group B Streptococcus Adult Invasive Disease in Manitoba and Saskatchewan, Canada, Is Driven by Clonal Sequence Type 459 Strains

et al. 2015

View full text Add to dashboard Cite

Serotype IV group B Streptococcus (GBS) is emerging in Canada and the United States with rates as high as 5% of the total burden of adult invasive GBS disease. To understand this emergence, we studied the population structure and assessed the antimicrobial susceptibility of serotype IV isolates causing adult invasive infection in Manitoba and Saskatchewan, Canada, between 2010 and 2014. Whole-genome sequencing was used to determine multilocus sequence typing information and identify genes encoding antimicrobial resistance in 85 invasive serotype IV GBS strains. Antimicrobial susceptibility testing was performed by standard methods. Strain divergence was assessed using genome-wide single-nucleotide polymorphism analysis. Serotype IV strains were responsible for 16.9% of adult invasive GBS infections in Manitoba and Saskatchewan during the period. The majority of serotype IV isolates (89%) were clonally related, tetracycline-, erythromycin-, and clindamycin-resistant sequence type 459 (ST459) strains that possessed genes tetM and ermTR. Genome comparisons between ST459 and serotype V ST1 GBS identified several areas of recombination in an overall similar genomic background. Serotype IV ST459 GBS strains are expanding and causing a substantial percentage of adult invasive GBS disease. This emergence may be linked to the acquisition of resistance to tetracycline, macrolides, and lincosamides.

show abstract

Section: Resultsmentioning

confidence: 99%

Emergence of Serotype IV Group B Streptococcus Adult Invasive Disease in Manitoba and Saskatchewan, Canada, Is Driven by Clonal Sequence Type 459 Strains

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The 41-kb integrative and conjugative element ICESpPS008 is nearly identical to ICESp2905 (6), although it harbors neither the erm(TR) nor the tet(O) antibiotic resistance gene and instead carries putative virulence factors, including genes encoding multidrug efflux proteins and a lipoprotein. Of note, ICESpPS008 is integrated at the 5= end of the RNA uracil-methyltransferase rum gene, a well-known integration hot spot for several mobile streptococcal elements (6).…”

Section: Resultsmentioning

confidence: 99%

Analysis of a Streptococcus pyogenes Puerperal Sepsis Cluster by Use of Whole-Genome Sequencing

et al. 2012

View full text Add to dashboard Cite

Between June and November 2010, a concerning rise in the number of cases of puerperal sepsis, a postpartum pelvic bacterial infection contracted by women after childbirth, was observed in the New South Wales, Australia, hospital system. Group A streptococcus (GAS; Streptococcus pyogenes) isolates PS001 to PS011 were recovered from nine patients. Pulsed-field gel electrophoresis and emm sequence typing revealed that GAS of emm1.40, emm75.0, emm77.0, emm89.0, and emm89.9 were each recovered from a single patient, ruling out a single source of infection. However, emm28.8 GAS were recovered from four different patients. To investigate the relatedness of these emm28 isolates, whole-genome sequencing was undertaken and the genome sequences were compared to the genome sequence of the emm28.4 reference strain, MGAS6180. A total of 186 single nucleotide polymorphisms were identified, for which the phylogenetic reconstruction indicated an outbreak of a polyclonal nature. While two isolates collected from different hospitals were not closely related, isolates from two puerperal sepsis patients from the same hospital were indistinguishable, suggesting patient-to-patient transmission or infection from a common source. The results of this study indicate that traditional typing protocols, such as pulsed-field gel electrophoresis, may not be sensitive enough to allow fine epidemiological discrimination of closely related bacterial isolates. Whole-genome sequencing presents a valid alternative that allows accurate fine-scale epidemiological investigation of bacterial infectious disease.

show abstract

“…Consistent with such heterogeneity of erm gene-encoded erythromycin resistance, a variety of erm-carrying genetic elements have been found and characterized in S. pyogenes, including diverse integrative and conjugative elements (ICEs) carrying erm(TR) (3,9,12) and closely related plasmids carrying erm(T) (11,33). As regards erm(B), it is carried by different elements depending on whether it is expressed constitutively or inducibly.…”

mentioning

confidence: 94%

ICE Sp1116 , the Genetic Element Responsible for erm (B)-Mediated, Inducible Resistance to Erythromycin in Streptococcus pyogenes

Brenciani

Tiberi

Morici

et al. 2012

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

bICESp1116, responsible for erm(B)-mediated, inducible erythromycin resistance in Streptococcus pyogenes, was comprehensively characterized, and its chromosomal integration site was determined. It displayed a unique mosaic organization consisting of a scaffold, related to TnGallo1 from Streptococcus gallolyticus, with two inserted fragments separated by IS1216. One fragment, containing erm(B), displayed high-level identity to a portion of the S. pyogenes plasmid pSM19035; the other, containing a truncated tet(M) gene, displayed high-level identity to the right-hand portion of Clostridium difficile Tn5397.

show abstract

Two Distinct Genetic Elements Are Responsible for erm (TR)-Mediated Erythromycin Resistance in Tetracycline-Susceptible and Tetracycline-Resistant Strains of Streptococcus pyogenes

Cited by 31 publications

References 28 publications

Emergence of Serotype IV Group B Streptococcus Adult Invasive Disease in Manitoba and Saskatchewan, Canada, Is Driven by Clonal Sequence Type 459 Strains

Emergence of Serotype IV Group B Streptococcus Adult Invasive Disease in Manitoba and Saskatchewan, Canada, Is Driven by Clonal Sequence Type 459 Strains

Analysis of a Streptococcus pyogenes Puerperal Sepsis Cluster by Use of Whole-Genome Sequencing

ICE Sp1116 , the Genetic Element Responsible for erm (B)-Mediated, Inducible Resistance to Erythromycin in Streptococcus pyogenes

Contact Info

Product

Resources

About