Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3

Tio, Phaik Hooi; Jong, Wan Wui; Cardosa, Mary Jane

doi:10.1186/1743-422x-2-25

Cited by 62 publications

(24 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sindbis virus uses LamR as a functional receptor to enter BHK-21 cells (39). Mosquito LamR was found to be a putative receptor for Venezuelan equine encephalitis virus (40) and dengue virus (41)(42)(43)(44). In mammalian cells, LamR acts as a cellular receptor for the cellular prion protein (PrP) (45,46), and it mediates the infection of adeno-associated virus serotypes 8, 2, 3, and 9 (47).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Laminin Receptor Is a Cellular Attachment Receptor for Classical Swine Fever Virus

Chen

Shen

et al. 2015

J Virol

View full text Add to dashboard Cite

Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious, economically important viral disease in many countries. The E rns and E2 envelope glycoproteins are responsible for the binding to and entry into the host cell by CSFV. To date, only one cellular receptor, heparan sulfate (HS), has been identified as being involved in CSFV attachment. HS is also present on the surface of various cells that are nonpermissive to CSFV. Hence, there must be another receptor(s) that has been unidentified to date. In this study, we used a set of small interfering RNAs (siRNAs) against a number of porcine cell membrane protein genes to screen cellular proteins involved in CSFV infection. This approach resulted in the identification of several proteins, and of these, the laminin receptor (LamR) has been demonstrated to be a cellular receptor for several viruses. Confocal analysis showed that LamR is colocalized with CSFV virions on the membrane, and a coimmunoprecipitation assay indicated that LamR interacts with the CSFV E rns protein. In inhibition assays, anti-LamR antibodies, soluble laminin, or LamR protein significantly inhibited CSFV infection in a dose-dependent manner. Transduction of PK-15 cells with a recombinant lentivirus expressing LamR yielded higher viral titers. Moreover, an attachment assay demonstrated that LamR functions during virus attachment. We also demonstrate that LamR acts as an alternative attachment receptor, especially in SK6 cells. These results indicate that LamR is a cellular attachment receptor for CSFV. IMPORTANCE Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), an economically important viral disease affecting the pig industry in many countries. To date, only heparan sulfate (HS) has been identified to be an attachment receptor for CSFV. Here, using RNA interference screening with small interfering RNAs (siRNAs) against a number of porcine membrane protein genes, we identified the laminin receptor (LamR) to be another attachment receptor. We demonstrate the involvement of LamR together with HS in virus attachment, and we elucidate the relationship between LamR and HS. LamR also serves as an attachment receptor for many viral pathogens, including dengue virus, a fatal human flavivirus. The study will help to enhance our understanding of the life cycle of flaviviruses and the development of antiviral strategies for flaviviruses. C lassical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious and often fatal viral disease in pigs. The disease leads to significant economic losses in many countries. CSFV is a member of the Pestivirus genus within the Flaviviridae family (1). The virus possesses a singlestranded, positive-sense RNA genome of approximately 12.3 kb (2, 3). Its genome contains a single, large open reading frame that encodes a precursor polyprotein of 3,898 amino acids (aa). The polyprotein is co-and posttranslationally processed by viral an...

show abstract

Section: Discussionmentioning

confidence: 99%

“…These membrane proteins were low-density lipoprotein receptor (LDLr), LamR, and vinculin. Among these, LamR has been identified to be a cellular receptor for different pathogens (38)(39)(40)(41)(42)(43)(44)(45)(46)(47). As shown in Fig.…”

Section: Membrane Proteins Required For Csfv Infection Were Screened mentioning

confidence: 99%

The Laminin Receptor Is a Cellular Attachment Receptor for Classical Swine Fever Virus

Chen

Shen

et al. 2015

J Virol

View full text Add to dashboard Cite

show abstract

“…This may suggest that dengue virus serotype 2 is capable of only binding to a denatured form of the laminin-binding protein. One other group has recently identifi ed the 37/67-kDa high-affi nity laminin receptor protein as a dengue virus-binding protein [31] . This group also showed that binding to the 37/67-kDa high-affi nity laminin receptor was serotype specifi c, and noted that dengue serotypes 1, 2 and 3, but not serotype 4, bound to the 37/ 67-kDa high-affi nity laminin receptor expressed by PS Clone D cells, raising the possibility that the binding between the dengue viruses and the 37/67-kDa high-affi nity laminin receptor may not only depend upon viral factors, but may also be infl uenced by specifi c posttranslational modifi cations of the 37/67-kDa high-affi nity laminin receptor.…”

Section: Discussionmentioning

confidence: 99%

Growth and Production of the Dengue Virus in C6/36 Cells and Identification of a Laminin-Binding Protein as a Candidate Serotype 3 and 4 Receptor Protein

Sakoonwatanyoo

Boonsanay

Smith³

2006

Intervirology

View full text Add to dashboard Cite

Objectives: Although dengue is one of the most common mosquito-borne viral diseases, few studies have investigated the relationship between the dengue virus and mosquito cells, and this study sought to describe the binding and propagation of the dengue viruses in C6/36 cells. Methods: The internalization and production of the dengue virus was assayed by standard plaque assay methodologies, while dengue virus receptor proteins were examined by a virus overlay protein-binding assay and candidate gene analysis coupled with virus inhibition studies. Results: All four serotypes were internalized linearly, and de novo virus production occurred 14–19 h postinfection. Virus overlay protein-binding assay identified a band of 50 kDa for dengue serotypes 2, 3 and 4 which comigrated with a protein that reacts with antibodies directed against the human 37/67-kDa high-affinity laminin receptor. Both antibodies directed against the human 37/67-kDa high-affinity laminin receptor protein and soluble laminin inhibited the binding and internalization of serotypes 3 and 4, but not serotypes 1 and 2. Conclusions: The results suggest that multiple receptors may be used by the dengue virus to enter into insect cells, and that one of these proteins may be a laminin-binding protein.

show abstract

“…Many studies have been carried out to identify molecules involved in dengue virus–host interactions. Independent studies have shown that different proteins, including high-affinity laminin receptor [10,11], CD14-associated protein [12], and other, uncharacterized, proteins [13] might be involved in dengue virus–host cell interactions. However, it has been reported that most of these molecules are not involved in dengue virus high-affinity interaction or internalization [14].…”

Section: Introductionmentioning

confidence: 99%

Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells

Aliyu

Ling

Hashim

et al. 2019

Viruses

View full text Add to dashboard Cite

Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediated inhibition of infection and siRNA-mediated knockdown of annexin II expression significantly reduced DENV2 infection and production levels. Collectively, we demonstrated that annexin II is one of the host factor involved in DENV2 binding on Vero cells.

show abstract

Two dimensional VOPBA reveals laminin receptor (LAMR1) interaction with dengue virus serotypes 1, 2 and 3

Cited by 62 publications

References 41 publications

The Laminin Receptor Is a Cellular Attachment Receptor for Classical Swine Fever Virus

The Laminin Receptor Is a Cellular Attachment Receptor for Classical Swine Fever Virus

Growth and Production of the Dengue Virus in C6/36 Cells and Identification of a Laminin-Binding Protein as a Candidate Serotype 3 and 4 Receptor Protein

Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells

Contact Info

Product

Resources

About