Two-dimensional thin-layer chromatography of Dns-amino acids on reversed-phase silica gel

Macek, Karel; Deyl, Z.; Smrž, M.

doi:10.1016/s0021-9673(00)87742-0

Cited by 26 publications

(1 citation statement)

References 9 publications

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“…Indeed, Crowshaw et al [25], Bhushan and Reddy [23] published extensive research on the separation of DNS amino acid derivatives in the normal phase system (silica gel). Still, the TLC/HPTLC reverse phase system is not popular/widely used for the separation of DNS derivatives [28], and it is specially difficult to find any publications concerning DNS aminoacids separation on RP-18W plates. However, there are more examples of the separation of DNS amino acids enantiomers using these plates [19,[29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Thin-layer chromatography of DNS amino acids derivatives in systems with silica gel and silanized silica gel plates

Chomicki

Dzido

2022

Current Issues in Pharmacy and Medical Sciences

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Investigations into separation selectivity of High-Performance Thin-layer chromatography (HPTLC) of dansyl (DNS) derivatives of amino acids in silica gel (Silica gel 60 F254s plates) and silanized silica gel (RP-18 W plates) systems are presented. The results have been obtained for mobile phases containing different concentrations of acetonitrile (ACN) in formic acid (FA) water solution (final concentration of FA in the mobile phase was equal to 265 mmol/dm3). The data obtained show differences in separation selectivity of the solutes between employment of HPTLC silica gel and RP-18 W systems.

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Section: Introductionmentioning

confidence: 99%

Thin-layer chromatography of DNS amino acids derivatives in systems with silica gel and silanized silica gel plates

Chomicki

Dzido

2022

Current Issues in Pharmacy and Medical Sciences

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Determination of enantiomeric purity of commercial 14C- and 3H-labeled L-α-amino acids

LeFevre

Bonzagni

Chappell

et al. 1998

J Label Compd Radiopharm

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The enantiomeric purity of twelve commercial 14C‐ and 3H‐labeled L‐α‐amino acids was determined using reverse isotope dilution analysis. The technique utilized reversed‐phase (RP) thin‐layer chromatography (TLC) and beta‐cyclodextrin (β‐CD) in the mobile phase to separate D‐ and L‐amino acids as their 5‐dimethylamino‐1‐naphtalene sulfonyl (dansyl, DNS) derivatives. In all cases, the L‐amino acid was contaminated with the D‐isomer. This is the first report of the resolution of N‐DNS‐Dl‐tyrosine and N‐(α)‐DNS‐Dl‐lysine using this methodology. © 1998 John Wiley & Sons, Ltd.

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Amino Acids

Drauz¹,

Höppe

Kleemann³

et al. 2001

Ullmann's Encyclopedia of Industrial Chemistry

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Two-dimensional thin-layer chromatography of Dns-amino acids on reversed-phase silica gel

Cited by 26 publications

References 9 publications

Thin-layer chromatography of DNS amino acids derivatives in systems with silica gel and silanized silica gel plates

Thin-layer chromatography of DNS amino acids derivatives in systems with silica gel and silanized silica gel plates

Determination of enantiomeric purity of commercial 14C- and 3H-labeled L-α-amino acids

Amino Acids

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