Two-Dimensional Micro-TLC Phenolic Fingerprints of SelectedMenthasp. on Cyano-Bonded Polar Stationary Phase

Abstract

Micro-thin-layer chromatography in two-dimensional (2D-mTLC) mode in normal- (NP) and reversed-phase (RP) systems by use of cyanopropyl-bonded stationary phases was applied to make fingerprints of 11 species of Mentha genus and two finished pharmaceutical products. Non-aqueous eluents were used in the NP systems. Mixtures of acetonitrile with water and methanol with water were used in the RP chromatographic systems. Optimization of one-dimensional systems was performed by determining RM vs. composition of mobile phase dependencies for standards occurring in various Mentha sp. On the basis of these dependencies, the most selective chromatographic systems for each run were chosen. Then most selective eluents were applied to optimize two-dimensional systems by creating RF in NP systems vs. RF in RP systems correlations. The best two-dimensional systems were chosen on the basis of R(2) values for RF vs. RF correlations (the lowest values of R(2) coefficients). The 2D-mTLC optimized systems were applied to separate phenolic compounds and make fingerprints of the examined plant materials.

Qualitative and Quantitative Analysis of Polyphenols in Lamiaceae Plants—A Review

“…Subsequently, improved MS analysis can be achieved as the matrix-associated ionization suppression is minimized [ 91 ]. In the research of Hawrył et al [ 48 ], a micro-2D-TLC method with cyanopropyl layers led to the separation of phenolic fractions from several mint species ( Mentha sp.) extracts ( Table 2 ).…”

“…Subsequently, the eluents with the higher selectivity were used to optimize the 2D systems through the development of R f (Retention factors) on the TLC plates, for both normal and reversed phases. It was noted that the 2D-TLC technique was highly sensitive, time efficient, and required low volumes of eluent and sample [ 48 ].…”

Isolation and characterization of two acaricidal compounds from Calpurnia aurea subsp. aurea (Fabaceae) leaf extract

“…In comparison, doxorubicin had LC 50 of 5.03±6.39 and 0.18±0.05 µM against Vero and HepG2 cells respectively (Table 3). (Nassar et al 2013;Boghrati et al 2016;Hawryl et al 2016).…”