Two‐dimensional liquid chromatography measurement of meropenem concentration in synovial fluid of patients with periprosthetic joint infection

Abstract

Periprosthetic joint infection (PJI) is a catastrophic complication following joint replacement surgery. One potential treatment approach for PJI could be the combination of one‐stage revision and intra‐articular infusion of antibiotics. Meropenem is one of the commonly used intra‐articular antibiotics in our institution. Determining the concentration of meropenem in the joint cavity could be crucial for optimizing its local application, effectively eradicating biofilm infection, and improving PJI treatment outcomes. In this study, we developed a simple, precise, and accurate method of two‐dimensional liquid chromatography (2D‐LC) for determining the concentration of meropenem in human synovial fluid. The method was then validated based on the guidelines of the Food and Drug Administration and the Chinese Pharmacopoeia. Meropenem showed good linearity in the range of 0.31–25.01 μg/mL (r ≥ .999). Selectivity, intra‐day and inter‐day precision and accuracy, extraction recovery, and stability validation results were all within the acceptance range. This method has been successfully applied to the determination of synovial fluid samples from PJI patients, providing a useful detection method for meropenem therapeutic drug monitoring (TDM) in PJI patients.