Two-Dimensional IgE-Binding Spectrum of Japanese Cedar &lt;i&gt;(Cryptomeria japonica)&lt;/i&gt; Pollen Allergens

Fujimura, Takashi; Shigeta, Seiko; Kawamoto, Seiji; Aki, Tsunehiro; Masubuchi, Masanori; Hayashi, Toshimitsu; Yoshizato, Katsutoshi; Ono, Kazuhisa

doi:10.1159/000076438

Cited by 32 publications

(45 citation statements)

References 40 publications

(43 reference statements)

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“…95% proteins other than Cry j 1 and Cry j 2. JC pollen contains a great variety of IgE-binding patterns (Fujimura et al, 2004) and numerous JC allergen components Role of DP 1 on JC Rhinitis in Mice other than Cry j 1 and Cry j 2, which might contribute to the pathology of JC pollinosis. Adjuvants such as alum and cholera toxin are usually used for the sensitization of animal models of allergic diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Previous investigations used an animal model of JC pollinosis sensitized with Cry j 1 or Cry j 2 (Murasugi et al, 2005;Nomiya et al, 2008); however, sera from JC pollinosis patients contain distinguishable immunoglobulin E (IgE)-binding patterns inlaid with 4-87 spots from a total of 131 IgE-binding protein spots detected by two-dimensional immunoelectrophoresis analysis (Fujimura et al, 2004), suggesting a large variation of IgE-binding patterns and that numerous JC allergens other than Cry j 1 and Cry j 2 might contribute to the pathophysiology of JC pollinosis.…”

Section: Introductionmentioning

confidence: 99%

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Role of Prostaglandin D2 and DP1 Receptor on Japanese Cedar Pollen-Induced Allergic Rhinitis in Mice

Nakano

Kidani

Goto

et al. 2016

Journal of Pharmacology and Experimental Therapeutics

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Although we previously demonstrated the contribution of the DP 1 receptor in nasal obstruction using animals sensitized with ovalbumin in the presence of adjuvant, the contribution of the DP 1 receptor in sneezing is unclear. Here, we developed a mouse model of Japanese cedar (JC: Cryptomeria japonica) pollinosis to evaluate the symptoms of sneezing. To achieve this, we used JC pollen crude extract in the absence of adjuvant to sensitize mice to develop a model closer to the pathophysiology of human JC pollinosis. The immunologic and pharmacologic features of this model are highly similar to those observed in JC pollinosis in humans. Using this model, we found that DP 1 receptor antagonists suppressed JC pollen extract-induced sneezing and that a DP 1 receptor agonist induced sneezing. Moreover, JC pollen extract-induced sneezing was diminished in DP 1 receptor knockout mice. In conclusion, we developed a novel mouse model of allergic rhinitis that closely mimics human JC pollinosis. A strong contribution of DP 1 receptor signaling to sneezing was demonstrated using this model, suggesting that DP 1 receptor antagonists could suppress sneezing and nasal obstruction, and therefore these agents could be a new therapeutic option for allergic rhinitis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of Prostaglandin D2 and DP1 Receptor on Japanese Cedar Pollen-Induced Allergic Rhinitis in Mice

Nakano

Kidani

Goto

et al. 2016

Journal of Pharmacology and Experimental Therapeutics

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“…Two-dimensional SDS-PAGE and allergenome analysis of D. farinae extract were performed using methods similar to those described previously (47). The two-dimensional immunoblotting analysis in the allergenomics study was carried out using plasma samples from 40 patients allergic to HDM.…”

Section: Methodsmentioning

confidence: 99%

Der f 34, a Novel Major House Dust Mite Allergen Belonging to a Highly Conserved Rid/YjgF/YER057c/UK114 Family of Imine Deaminases

ElRamlawy

Fujimura

Baba

et al. 2016

Journal of Biological Chemistry

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The high prevalence of house dust mite (HDM) allergy is a growing health problem worldwide, and the characterization of clinically important HDM allergens is a prerequisite for the development of diagnostic and therapeutic strategies. Here, we report a novel HDM allergen that belongs structurally to the highly conserved Rid/YjgF/YER057c/UK114 family (Rid family) with imine deaminase activity. Isolated HDM cDNA, named der f 34, encodes 128 amino acids homologous to Rid-like proteins. This new protein belongs to the Rid family and has seven conserved residues involved in enamine/imine deaminase activity. Indeed, we demonstrated that purified Der f 34 had imine deaminase activity that preferentially acted on leucine and methionine. Native Der f 34 showed a high IgE binding frequency as revealed by two-dimensional immunoblotting (62.5%) or ELISA (68%), which was comparable with those of a major HDM allergen Der f 2 (77.5 and 79%, respectively). We also found that Der f 34 showed cross-reactivity with another prominent indoor allergen source, Aspergillus fumigatus. This is the first report showing that the Rid family imine deaminase represents an additional important pan-allergen that is conserved across organisms.Asthma is a serious global health problem that significantly reduces quality of life. In a cross-sectional World Health Organization survey of 178,215 individuals from 70 countries conducted in 2002-2003, it was estimated that 4.3% of adults (range, 0.2-21.0%) had been diagnosed with asthma by a medical doctor (1). Inhaled airborne allergens cause allergic inflammation via the cross-linking of allergen-specific IgEs bound on high affinity IgE receptor (FceRI) located on the surface of airway or lung mast cells, causing the secretion of inflammatory chemical mediators (e.g. histamine and leukotrienes) from activated mast cells. House dust mites (HDMs) 5 and fungi are major sources of airborne allergens and trigger asthmatic attacks in allergic patients concomitant with air pollutants (2).Allergen-specific immunotherapy (AIT) is the only curative treatment for allergic asthma that changes the natural course of an allergy (3). AIT induces allergen-specific tolerance by administering increasing doses of causative allergens subcutaneously or sublingually (4). The precise mechanisms underlying AIT remain to be fully elucidated, but the involvement of regulatory T cells and B cells or the induction of T cell anergy is suggested to be a key mechanism for an efficient AIT. AIT induces antigen-specific immunomodulatory cells or the anergic response of T cells specific to major allergens included in an allergen extract as an AIT vaccine. Therefore, the standardization of AIT vaccines using important major allergens and the characterization of major component allergens in an allergen source are important issues for the development of an effective AIT (5, 6).

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“…The Cry j 1 protein appears to be produced in the tapetum and the Golgi bodies of mature pollen and is localized in the orbicles and pollen wall [8]. Two-dimensional immunolabeling with IgE from sera of patients with pollinosis revealed the presence of at least 12 isoforms of Cry j 1 [9]. Four cDNAs that include entire open reading frames of Cry j 1 isoforms have been reported, and the isoforms have different reactivities to two monoclonal antibodies [10].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Genes for a Pollen Allergen, Cry j 2, of <i>Cryptomeria japonica</i>

Futamura

Kusunoki²,

Mukai³

et al. 2006

Int Arch Allergy Immunol

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Background: Cry j 2 is one of the major pollen allergens of Cryptomeria japonica. The polymorphism of Cry j 2 isoforms and the conservation of the structure of Cry j 2 in coniferous species remain to be analyzed. Methods:A cDNA library derived from the pollen of C. japonica was screened using a fragment of Cry j 2 cDNA. Restriction fragment length polymorphism analysis was performed to examine the diversity of Cry j 2 genes. The promoters of Cry j 2 genes were isolated with a commercially available cloning kit. Clonal variations in the expression of Cry j 2 in pollen were examined by RNA gel blot analysis, and the conservation of the structure of the Cry j 2 gene in coniferous species was evaluated by DNA gel blot analysis. Results: We isolated three cDNA clones encoding novel isoforms of Cry j 2. We also sequenced a total of 16 promoter regions from 10 specimens. The sequences of promoter regions of Cry j 2 genes were highly divergent. The amount of Cry j 2 mRNA also varied considerably. The Cry j 2 gene was found to be conserved among species belonging to Taxodiaceae and Cupressaceae but to vary between Taxodiaceae and Pinaceae. Conclusions: The coding and promoter regions of Cry j 2 genes contain large numbers of polymorphisms. Our analysis revealed large variations in the expression of Cry j 2 at the transcriptional level, and we suggest that conserved homologs of Cry j 2 confer cross-allergenicity among Taxodiaceae and Cupressaceae.

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Two-Dimensional IgE-Binding Spectrum of Japanese Cedar <i>(Cryptomeria japonica)</i> Pollen Allergens

Cited by 32 publications

References 40 publications

Role of Prostaglandin D2 and DP1 Receptor on Japanese Cedar Pollen-Induced Allergic Rhinitis in Mice

Role of Prostaglandin D2 and DP1 Receptor on Japanese Cedar Pollen-Induced Allergic Rhinitis in Mice

Der f 34, a Novel Major House Dust Mite Allergen Belonging to a Highly Conserved Rid/YjgF/YER057c/UK114 Family of Imine Deaminases

Characterization of Genes for a Pollen Allergen, Cry j 2, of <i>Cryptomeria japonica</i>

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