Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing

Dorri, Yaser

doi:10.1007/978-1-4939-8793-1_25

Cited by 3 publications

(3 citation statements)

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“…Only annexin B1 and cAMP-dependent protein kinase are highly specific and may be developed as candidate diagnostic antigens for cysticercosis. As the primary method for separating and comparing protein mixtures, 2-dimensional electrophoresis (2-DE) combined with mass spectrometry (MS) has become a major tool for studying proteomics due to its high resolution, and it is often used to construct differential protein expression profiles (Dorri, 2019). The method separates proteins according to their isoelectric point in the first and second dimensions according to their molecular weight.…”

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confidence: 99%

Proteomic Analysis of Taenia solium Cyst Fluid by Shotgun LC-MS/MS

Cui

Wang

et al. 2021

Journal of Parasitology

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confidence: 99%

Proteomic Analysis of Taenia solium Cyst Fluid by Shotgun LC-MS/MS

Cui

Wang

et al. 2021

Journal of Parasitology

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“…Protein samples were separated via standard SDS‐PAGE or 2‐DE that used vertical isoelectric focusing PAGE for the first dimension . The gels were imaged with a LI‐COR Odyssey imaging system (LI‐COR, Inc, Lincoln, NE) with settings as a dual channel (700 and 800 nm; the 700‐nm channel was monitored for visualizing the reference protein ladder), a focus offset of 0.75 mm for 1.5‐mm‐thick gel or 0.5 mm for 1.0‐mm‐thick gel, 7.0 intensity, and highest quality.…”

Section: Methodsmentioning

confidence: 99%

Treasure hunt for peptides with undefined chemical modifications: Proteomics identification of differential albumin adducts of 2‐nitroimidazole‐indocyanine green in hypoxic tumor

et al. 2019

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2‐Nitroimidazole is a well‐known chemical probe targeting hypoxic environments of solid tumors, and its derivatives are widely used as imaging agents to investigate tissue and tumor hypoxia. However, the underlying chemistry for the hypoxia‐detection capability of 2‐nitroimidazole is still unclear. In this study, we deployed a biotin conjugate of 2‐nitroimidazole‐indocyanine green (2‐nitro‐ICG) for the investigation of in vivo hypoxia‐probing mechanism of 2‐nitro‐ICG compounds. By implementing mass spectrometry‐based proteomics and exhaustive data mining, we report that 2‐nitro‐ICG and its fragments modify mouse serum albumin as the primary protein target but at two structurally distinct sites and possibly via two different mechanisms. The identification of probe‐modified peptides not only contributes to the understanding of the in vivo metabolism of 2‐nitroimidazole compounds but also demonstrates a competent analytical workflow that enables the search for peptides with undefined modifications in complex proteome digests.

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“…Numerosos protocolos de laboratorio son utilizados para identificar proteínas, siendo la técnica electroforesis 2D, importante para la separación de proteínas por su peso molecular (PM) y carga eléctrica [14], siendo la técnica muy utilizada en la determinación de la composición proteica de muestras biológicas, además de ser utilizada desde hace algunos años para determinar la presencia del lipopolisacárido (LPS). Es una técnica que demostró ser altamente sensible; es más sencilla que las de resonancia magnética nuclear (RMN) y cromatografía gaseosa acoplada a masas, no requiere de complejos equipamientos y por lo general en los laboratorios de control de proceso de vacunas se cuenta con el equipamiento y los reactivos necesarios para su utilización [9].…”

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Perfil electroforético 2D de las proteínas del intestino de Fasciola hepatica

et al. 2022

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Siendo la fasciolosis una infección parasitaria importante en rumiantes de muchos países y dada la alta prevalencia en humanos y animales en Cajamarca, Perú, se planteó realizar el estudio sobre el perfil de las proteínas de intestino de Fasciola hepatica con el objetivo de conocer el número de proteínas y el rango de pH de secreción/excreción de intestino del parásito que expresa mediante el método de electroforesis 2D-bidimensional. Las muestras adultas de F. hepatica se recolectaron de hígados de bovinos en el Camal Municipal de Cajamarca. Fueron trasladadas al laboratorio de Biotecnología en Sanidad Animal de la Estación Experimental Agraria Baños del Inca, INIA – Cajamarca, para su procesamiento. La corrida electroforética permitió separar 82 proteínas con diferentes pesos moleculares, enfocadas en distintos puntos isoeléctricos en un rango de pH de 6,0 a 9,4. Se concluye que mediante el análisis del gel 2D de proteínas de intestino de F. hepatica, se conocieron 84 spots de proteínas con distintos pesos moleculares, enfocadas en distintos puntos isoeléctricos en un rango de 6,0 a 9,4.

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Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing

Cited by 3 publications

References 10 publications

Proteomic Analysis of Taenia solium Cyst Fluid by Shotgun LC-MS/MS

Proteomic Analysis of Taenia solium Cyst Fluid by Shotgun LC-MS/MS

Treasure hunt for peptides with undefined chemical modifications: Proteomics identification of differential albumin adducts of 2‐nitroimidazole‐indocyanine green in hypoxic tumor

Perfil electroforético 2D de las proteínas del intestino de Fasciola hepatica

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