Two-dimensional electrophoretic analysis of proteins associated with somatic embryogenesis development inCupressus sempervirens L.

Sallandrouze, Anne; Faurobert, Mireille; Maataoui, Mohammed El; Espagnac, H.

doi:10.1002/(sici)1522-2683(19990101)20:4/5<1109::aid-elps1109>3.0.co;2-4

Cited by 35 publications

(3 citation statements)

References 35 publications

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“…In addition, SERK was detected during flower development and was highest 10 days after pollination [173]. Homologs of SERK have been discovered in several plant species, which includes Dactylis glomerata [184], Zea mays [5], Medicago truncatula [130], Helianthus annuus [198], Oryza sativa [79], Citrus unshiu [178], Theobroma cacao [169,170], and Solanum tuberosum [177]. Analysis of the sequenced Arabidopsis genome revealed that a family of five homologs of the carrot SERK was present.…”

Section: Serk Genesmentioning

confidence: 56%

“…Later in embryogenesis, lec2 mutants have cotyledon tips that do not accumulate storage reserves nor acquire desiccation tolerance, indicating defects in the initiation and/or maintenance of the maturation phase. Consistent with the pleiotropic effects of the lec2 mutation, LEC2 encodes a transcription factor with a B3 domain, a DNA binding region found thus far only in plant proteins [169,170,191].…”

Section: Lec Genesmentioning

confidence: 99%

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Molecular aspects of somatic-to-embryogenic transition in plants

2009

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Somatic embryogenesis (SE) is a model system for understanding the physiological, biochemical, and molecular biological events occurring during plant embryo development. Plant somatic cells have the ability to undergo sustained divisions and give rise to an entire organism. This remarkable feature is called plant cell totipotency. SE is a notable illustration of plant totipotency and involves reprogramming of development in somatic cells toward the embryogenic pathway. Plant growth regularities, especially auxins, are key components as their exogenous application recapitulates the embryogenic potential of the mitotically quiescent somatic cells. It has been observed that there are genetic and also physiological factors that trigger in vitro embryogenesis in various types of plant somatic cells. Analysis of the proteome and transcriptome has led to the identification and characterization of certain genes involved in SE. Most of these genes, however, are upregulated only in the late developmental stages, suggesting that they do not play a direct role in the vegetative-to-embryogenic transition. However, the molecular bases of those triggering factors and the genetic and biochemical mechanisms leading to in vitro embryogenesis are still unknown. Here, we describe the plant factors that participate in the vegetative-to-embryogenic transition and discuss their possible roles in this process.

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Section: Serk Genesmentioning

confidence: 56%

Section: Lec Genesmentioning

confidence: 99%

Molecular aspects of somatic-to-embryogenic transition in plants

2009

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“…There are several proteomics reports dealing with SE in different plant species e.g. Daucus carota [33], Oryza sativa L. [34], Camellia japonica [35], Cupressus sempervirens L. [36], Spinacia oleracea [37], Vitis vinifera [38], Medicago truncatula [7,39], Cyclamen persicum [40-42], Picea glauca [43], Citrus sinensis Osbeck [44,45], and Acca sellowiana [46]. Proteomic analyses provide new insights into the molecular basis of SE and exploring some black boxes of this process, pave the way for future in vitro scale up propagation and genetic manipulation through the development and optimization of strategies for efficient somatic embryo production.…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.)

et al. 2012

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BackgroundSomatic embryogenesis (SE) is a complex biological process that occurs under inductive conditions and causes fully differentiated cells to be reprogrammed to an embryo like state. In order to get a better insight about molecular basis of the SE in Crocus sativus L. and to characterize differentially accumulated proteins during the process, a proteomic study based on two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry has been carried out.ResultsWe have compared proteome profiles of non-embryogenic and embryogenic calli with native corm explants. Total soluble proteins were phenol-extracted and loaded on 18 cm IPG strips for the first dimension and 11.5% sodium dodecyl sulfate-polyacrylamide gels for the second dimension. Fifty spots with more than 1.5-fold change in abundance were subjected to mass spectrometry analysis for further characterization. Among them 36 proteins could be identified, which are classified into defense and stress response, protein synthesis and processing, carbohydrate and energy metabolism, secondary metabolism, and nitrogen metabolism.ConclusionOur results showed that diverse cellular and molecular processes were affected during somatic to embryogenic transition. Differential proteomic analysis suggests a key role for ascorbate metabolism during early stage of SE, and points to the possible role of ascorbate-glutathione cycle in establishing somatic embryos.

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Plant Proteomics

Saravanan¹,

Bashir²,

Rose³

2004

Handbook of Plant Biotechnology

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Proteomics, or the study of the protein complement of the genome, promises to help bridge the divide between genomic DNA sequence and biological state and represents one of the most rapidly developing and innovative fields in genome‐scale research. The term is now commonly and broadly used to encompass almost any aspect of protein structure, expression and function. However, as used here it refers to characterising quantitative and qualitative characteristics of global protein expression, with an emphasis on scale, including polypeptide synthesis, degradation, post‐translational modification, the structure of protein complexes and interactions with other proteins and cellular components. The systematic characterisation of complex protein populations can be pursued using many approaches and numerous reviews have recently been published describing developments in diverse areas of proteomics, including protein structure, function and protein–protein, or protein–ligand interactions A number of reviews have also been specifically devoted to plant proteomics. Consequently, the multiplicity of proteomics‐related experimentation and developing technologies are not reiterated here in detail. Instead, this chapter intends to provide an overview of the diversity of proteomics‐based studies that have been reported in plants to date and emerging technologies, which in turn should underscore the tremendous potential utility of proteomics for plant biologists and give an indication of future applications.

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Two-dimensional electrophoretic analysis of proteins associated with somatic embryogenesis development inCupressus sempervirens L.

Cited by 35 publications

References 35 publications

Molecular aspects of somatic-to-embryogenic transition in plants

Molecular aspects of somatic-to-embryogenic transition in plants

Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.)

Plant Proteomics

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