Two-dimensional electrophoresis map of the human hepatocellular carcinoma cell line, HCC-M, and identification of the separated proteins by mass spectrometry

Seow, Teck Keong; Ong, Shao En; Liang, Rosa C. M. Y.; Ren, Ee Chee; Chan, Lily; Ou, Keli; Chung, Maxey C. M.

doi:10.1002/(sici)1522-2683(20000501)21:9<1787::aid-elps1787>3.0.co;2-a

Cited by 124 publications

(39 citation statements)

References 38 publications

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“…33,34 In addition, a 2D-PAGE of the hepatoma cell line HCC-M identified prohibitin as a positively regulated protein. 35 In these studies, the mechanism of prohibitin overexpression was not elucidated, but considering that prohibitin is associated with the inhibition of cell proliferation, the function of prohibitin is suppressed by stabilization by some molecules in the tumor, similar to the mechanism we suggest in the current study.…”

Section: Discussionsupporting

confidence: 70%

Proteomics analysis of mitochondrial proteins reveals overexpression of a mitochondrial protein chaperon, prohibitin, in cells expressing hepatitis C virus core protein

et al. 2009

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Section: Discussionsupporting

confidence: 70%

Proteomics analysis of mitochondrial proteins reveals overexpression of a mitochondrial protein chaperon, prohibitin, in cells expressing hepatitis C virus core protein

et al. 2009

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“…45 Enhanced expression of annexin A1 was observed in poorly differentiated HCC tissues compared with their well-differentiated counterparts, and the nontumorous region contained significantly lower amounts of annexin A1, suggesting that annexin A1 is related to the histological grade of HCC and is involved in the malignant transformation process. 36 The histological undifferentiation observed in AFP-positive HCC may be explained by the enhanced expression of annexin A1.…”

Section: Discussionmentioning

confidence: 96%

Proteomic signature corresponding to alpha fetoprotein expression in liver cancer cells

et al. 2004

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Alpha fetoprotein (AFP) has been implicated in the development of hepatocellular carcinoma and is considered to be a diagnostic and prognostic tumor marker. Because elevated expression of AFP is associated with many characteristics of hepatocellular carcinoma tissues, we hypothesized that multiple proteins may function in a coordinated manner with AFP. To identify such proteins, we performed global protein expression analysis, namely a proteomic study. The protein expression profiles of 9 AFP-producing liver cancer cell lines (JHH-5, HuH-1, PLC/PRL/5, Hep3B, HT-17, JHH-7, HuH-7, HepG2, Li-7) and 7 nonproducing liver cancer cell lines ( H epatocellular carcinoma (HCC) represents the vast majority of liver cancer, accounting for more than 90% of all primary liver cancers, and ranks fifth in frequency among all malignancies worldwide. 1,2 Infection with hepatitis B or C virus has been identified as an etiological factor, and the subsequent cellular and histological changes leading to HCC have been extensively studied. 3 Genetic alterations, including loss of heterozygosity 4,5 and aberrant DNA methylation, 6 also have been implicated in the carcinogenesis of HCC. Nevertheless, the outcome for HCC patients still remains dismal, partly because of the difficulty in establishing an early diagnosis and the frequent recurrence and intrahepatic metastasis after surgery. 7 Although systematic chemotherapy for unresectable HCC has been widely used, its efficacy remains low and complications such as significant myelosuppression are observed during the course of treatment. 8 Serum alpha fetoprotein (AFP) has been considered to be a hallmark of development of HCC. 9 Serum AFP alone has a limited role in the early diagnosis of HCC, because a considerable proportion of HCC patients do not have elevated serum AFP, and serum AFP can increase in patients with diseases other than HCC; it may, however, be a useful prognostic indicator, because the median survival rate of HCC patients with markedly elevated AFP was significantly shorter than that of patients with normal or moderately elevated AFP. 10 AFP is a multifunctional glycoprotein belonging to the family of albumin-like proteins. 11 AFP has been shown to serve as a dual regulator of growth in a multitude of cell types and cancers involving

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“…Thus, their properties in drug resistance, apoptosis, and invasiveness may be different. For structural proteins, laminin receptor and annexin II are upregulated in HCC cells, and these two proteins were also expressed in HCC-M cell lines (11,20). There is much evidence to indicate that the 67-kDa laminin receptor is important in the progression of a wide variety of carcinomas (72).…”

Section: Discussionmentioning

confidence: 99%

Comparison of protein expression patterns between hepatocellular carcinoma cell lines and a hepatoblastoma cell line

et al. 2004

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Hepatocellular carcinoma (HCC) and hepatoblastoma (HB) are malignancies of the liver with different etiologies, but the HB cell line HepG2 has been frequently used in various studies of HCC. In this study, we compare the protein expression patterns between HepG2 cells and three HCC cell lines, HKCI-2, HKCI-3, and HKCI-4, respectively. The cell lysates of individual cell lines were separated by twodimensional polyacrylamide gel electrophoresis. The protein spots in the gel images were quantified and compared by image analysis software. The differentially expressing proteins were then identified by tryptic peptide mass fingerprinting. Compared with the HepG2 cells, the normalized quantities of 49 and 58 protein spots were found to be at least twofold higher and twofold lower, respectively, in all three HCC cell lines. The differentially expressed proteins can be grouped into structural proteins (annexins, transgelin, laminin receptor), stress-induced proteins (HSP27, 60, and 70), enzymes (aldehyde dehydrogenase, pyruvate kinase, ␣-enolase, etc.), and transcription factors (far upstream element binding protein 2, GTP-binding nuclear protein RAN).

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Two-dimensional electrophoresis map of the human hepatocellular carcinoma cell line, HCC-M, and identification of the separated proteins by mass spectrometry

Cited by 124 publications

References 38 publications

Proteomics analysis of mitochondrial proteins reveals overexpression of a mitochondrial protein chaperon, prohibitin, in cells expressing hepatitis C virus core protein

Proteomics analysis of mitochondrial proteins reveals overexpression of a mitochondrial protein chaperon, prohibitin, in cells expressing hepatitis C virus core protein

Proteomic signature corresponding to alpha fetoprotein expression in liver cancer cells

Comparison of protein expression patterns between hepatocellular carcinoma cell lines and a hepatoblastoma cell line

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