Two-dimensional electrophoresis analyses of atopic dermatitis and the chances to detect new candidate proteins by the variations in immobilized pH gradient strips

Park, Yong‐Doo; Lyou, You-Jeong; Yang, Jun‐Mo

doi:10.1016/j.jdermsci.2007.02.004

Cited by 18 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, some studies reported that NPM may play a role in AD. Park et al 57 used two-dimensional electrophoresis to determine that NPM expression was altered in cases of AD. Interestingly, the effects of WA-25 on AD were disrupted by either NSC 348884 or NPM siRNA.…”

Section: Discussionmentioning

confidence: 99%

Nucleophosmin modulates the alleviation of atopic dermatitis caused by the marine-derived compound dihydroaustrasulfone alcohol

et al. 2018

View full text Add to dashboard Cite

Atopic dermatitis (AD) is a chronic inflammatory skin disease, and its prevalence is increasing. AD usually elicits skin barrier dysfunction, dry skin and itching. As the mechanisms of AD remain unknown, there is an urgent need to find effective therapies. Because of the diversity and complexity of marine environments, the discovery of drugs from marine organisms as novel therapeutic agents for human diseases has seen renewed interest. Dihydroaustrasulfone alcohol (WA-25), the synthetic precursor of austrasulfone, which is a natural product isolated from a Formosan soft coral, has been shown to possess many therapeutic effects in our previous studies. However, the detailed mechanisms and therapeutic effects of WA-25 on AD are incompletely understood. We performed in vitro and in vivo studies to examine the effects of WA-25 on AD. We showed that WA-25 blocks inflammation and oxidative stress. Simultaneously, we also found that WA-25 reduces the AD scores and AD-induced transepidermal water loss (TEWL), scratching behavior, and alloknesis. WA-25 is more effective in cases of AD than are the drugs that are currently used clinically. Importantly, we also found that when nucleophosmin (NPM) was inhibited or when its expression was reduced, the anti-inflammatory and anti-AD effects of WA-25 were blocked. These data suggest that NPM plays dual roles in inflammation and AD. Overall, these results suggest that WA-25 is a potential anti-inflammatory and AD therapeutic agent that is modulated by NPM.

show abstract

Section: Discussionmentioning

confidence: 99%

Nucleophosmin modulates the alleviation of atopic dermatitis caused by the marine-derived compound dihydroaustrasulfone alcohol

et al. 2018

View full text Add to dashboard Cite

show abstract

“…CLIC4 has been implicated in a variety of cellular functions including angiogenesis, apoptosis, keratinocyte and adipocyte differentiation, LPS-mediated innate immunity, macrophage deactivation and TGF-Î² signaling [10,13,17-21]. Changes in CLIC4 expression or subcellular localization are also associated with several pathological conditions including cancer, atopic dermatitis, ethylmalonic encephalopathy, alcoholism and autism, but the functional significance of these changes is still not well defined [20,22-25]. Mice lacking CLIC4 are viable and three independent groups have generated CLIC4 knockout mice and reported differing phenotypic outcomes implicating CLIC4 in endothelial tubule formation, skin and corneal wound healing and innate immunity [18,21,26].…”

Section: Introductionmentioning

confidence: 99%

Detection of differential fetal and adult expression of chloride intracellular channel 4 (CLIC4) protein by analysis of a green fluorescent protein knock-in mouse line

et al. 2014

View full text Add to dashboard Cite

BackgroundChloride Intracellular Channel 4 (CLIC4) is one of seven members in the closely related CLIC protein family. CLIC4 is involved in multiple cellular processes including apoptosis, cellular differentiation, inflammation and endothelial tubulogenesis. Despite over a decade of research, no comprehensive in situ expression analysis of CLIC4 in a living organism has been reported. In order to fulfill this goal, we generated a knock-in mouse to express Green Fluorescent Protein (GFP) from the CLIC4 locus, thus substituting the GFP coding region for CLIC4. We used GFP protein expression to eliminate cross reaction with other CLIC family members.ResultsWe analyzed CLIC4 expression during embryonic development and adult organs. During mid and late gestation, CLIC4 expression is modulated particularly in fetal brain, heart, thymus, liver and kidney as well as in developing brown adipose tissue and stratifying epidermis. In the adult mouse, CLIC4 is highly expressed globally in vascular endothelial cells as well as in liver, lung alveolar septae, pancreatic acini, spermatogonia, renal proximal tubules, cardiomyocytes and thymic epithelial cells. Neural expression included axonal tracks, olfactory bulb, Purkinje cell layer and dentate gyrus. Renal CLIC4 expression was most pronounced in proximal tubules, although altered renal function was not detected in the absence of CLIC4. Myeloid cells and B cells of the spleen are rich in CLIC4 expression as are CD4 and CD8 positive T cells.ConclusionsIn a comprehensive study detailing CLIC4 expression in situ in a mouse model that excludes cross reaction with other family members, we were able to document previously unreported expression for CLIC4 in developing fetus, particularly the brain. In addition, compartmentalized expression of CLIC4 in specific adult tissues and cells provides a focus to explore potential functions of this protein not addressed previously.

show abstract

“…Both proteomic and gene expression profilings of AD fibroblasts were carried out by Park et al They investigated alterations in protein expression in primary cultured AD cells from ADe and ADi biopsy samples by classical 2-DE and MALDI-TOF [54] and additionally extended their analysis with the use of different pH gradients [55] to increase the possibility to detect protein markers. They also reported, notably, that PTMs of candidate proteins may be relevant in the development of the disease [54].…”

Section: Atopic Dermatitismentioning

confidence: 99%

Post-Genomics and Skin Inflammation

Braconi

Bernardini

Santucci

2010

Mediators of Inflammation

View full text Add to dashboard Cite

Atopic dermatitis and psoriasis are two chronic skin inflammatory diseases that have so far received a greater attention within the scientific community through different post-genomic approaches; on the contrary, acne, which is undoubtedly one of the most common skin disorders involving inflammatory processes, seems to be still quite neglected under the post-genomic point of view. In this paper, we will review how post-genomic technologies have provided new fundamental tools for the analysis of these three conditions and we will cast light on their potential in addressing future research challenges.

show abstract

Two-dimensional electrophoresis analyses of atopic dermatitis and the chances to detect new candidate proteins by the variations in immobilized pH gradient strips

Cited by 18 publications

References 22 publications

Nucleophosmin modulates the alleviation of atopic dermatitis caused by the marine-derived compound dihydroaustrasulfone alcohol

Nucleophosmin modulates the alleviation of atopic dermatitis caused by the marine-derived compound dihydroaustrasulfone alcohol

Detection of differential fetal and adult expression of chloride intracellular channel 4 (CLIC4) protein by analysis of a green fluorescent protein knock-in mouse line

Post-Genomics and Skin Inflammation

Contact Info

Product

Resources

About