Two‐dimensional capillary gas chromatography without intermediate trapping. Electron capture detector quantitation of an amino alcohol (KABI 2128) in serum after trifluoroacetylation

Abstract

A two‐ dimensional gas chromatograph based on the Deans switching principle is described. The unit comprises two separate ovens, each containing a fused silica capillary column. The columns are joined in a specially designed manifold permitting heart cuts to be performed without significant decrease in efficiency. The switching speed and the retention time stability of the system made it possible to perform heart cuts of only a few seconds' duration. The system has been used under isothermal conditions for the determination of an amino alcohol (KABI 2128) in the low ng/ml range after trifluoroacetylation and with electron capture detection. A much shorter clean‐up procedure could be used in combination with the two‐dimensional gas chromatograph as compared to a method using a single glass capillary column.

Multidimensional gas chromatography: State of the art

“…On the other hand, shortened total analysis times have been reported. In the analysis of an amino alcohol from serum samples by G C G C , the total analysis time could be reduced to 15 minutes from 50 minutes by conventional gas chromatography (51). The need for sample pretreatment was diminished to mere derivatization after extraction (Figure 4).…”

Aspects of High-Resolution Gas Chromatography as Applied to the Analysis of Hydrocarbon Fuels and Other Complex Organic Mixtures. Volume 1

“…A paper recently presented discussed some of the requirements to convert a conventional gas Chromatographie system to a MDGC system [234]. Work has also been conducted with MDGC systems which incorporate the use of intermediate trapping [235] and a two-dimensional capillary system which did not use intermediate trapping [236]. Some very interesting recent work [237,233] has involved MDGC systems which emphasize the use of two separate ovens with different temperature versus time histories for obtaining Chromatographie separations.…”