Two-dimensional C<sub>3</sub>N based sub-10 nanometer biosensor

Rani, Shivani; Ray, S. J.

doi:10.1039/d0cp00546k

Cited by 21 publications

(13 citation statements)

References 47 publications

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“…Also, the folic-acid-modified C 3 N dots can be selectively endocytosed by tumor cells, which finally lead the cell to die . Similarly, 2D C 3 N could be utilized as a biosensor . Despite their diverse applications in biological systems, so far there are very limited studies or evidences to disclose the interaction dynamics and/or potential molecular mechanism of biomolecules binding to carbon nitride compounds.…”

Section: Introductionmentioning

confidence: 99%

Partial Denaturation of Villin Headpiece upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet

Pérez-Aguilar

Meng

et al. 2020

J. Phys. Chem. B

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Carbon nitride polyaniline (C3N) nanosheets, since their recent successful synthesis, have been explored for biomedical applications. However, a thorough study of their interaction with biomolecules is still largely missing. Here, by using all-atom molecular dynamics simulations, we identified the mechanistic determinants of the interaction between a C3N nanosheet and the prototypical protein villin headpiece (HP35). Our simulations revealed that, upon adsorption, the nanosheet can cause partial denaturation of HP35 by destructing its interior hydrogen bonds plus other native contacts and unwinding its helices. Our study also demonstrated that the C3N/HP35 interaction energy showed stepwise changes during the binding process and held a strong correlation with the loss of HP35 native contacts. The findings shed light on the detailed molecular mechanism behind the interactions, which might benefit the future applications of C3N-based nanostructures in biomedicine.

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Section: Introductionmentioning

confidence: 99%

Partial Denaturation of Villin Headpiece upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet

Pérez-Aguilar

Meng

et al. 2020

J. Phys. Chem. B

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“…The two-dimensional carbon nitride C 3 N (also named as polyaniline) nanosheet composed of the basic unit of a benzene ring surrounded by six nitrogen atoms, which was experimentally polymerized by anilines (as illustrated in Figure a). − Herein, we employed a dsDNA segment with the sequence (ATCG) 4 as a model to investigate the potential effect of the C 3 N nanosheet to dsDNA. Two systems were constructed as displayed in Figure b,c with the orientation of dsDNA being rotated (perpendicular and parallel to C 3 N nanosheet), and each of them was studied by three independent 500 ns-length atomistic MD simulations.…”

Section: Resultsmentioning

confidence: 99%

“…32 In addition, 2D C 3 N could be utilized as a biosensor for the sequencing of various nucleobases. 33 Despite their comprehensive applications in biomedicine, there were by far very few studies or evidence to disclose the effect of C 3 N nanomaterials to biomolecules. Our recent work probed the adhesion of the prototypical protein villin headpiece (HP35) to a C 3 N nanosheet and proved that the protein's structural integrity was partially denaturized during the adsorption, implying the possible harmfulness of C 3 N nanomaterials.…”

Section: ■ Introductionmentioning

confidence: 99%

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Potential Unwinding of Double-Stranded DNA upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet

Zhao

2021

J. Phys. Chem. B

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Recently, carbon nitride polyaniline (C3N) had attracted considerable attention from many scientific fields after its successful synthesis. However, thus far, limited efforts were devoted to reveal its potential effect to biomolecules, which correlated intimately with its further utilization. In this study, by using a molecular dynamics (MD) simulation approach, we investigated in detail the interaction between C3N and a double-stranded DNA (dsDNA) segment to expose the underlying biological effect of C3N to dsDNA and the corresponding molecular basis. MD simulation results demonstrated that dsDNA presented serious damages upon adsorption onto a C3N nanosheet with the terminal base pairs denaturized, unwound, and directly packing on the C3N surface, which implied that C3N was potentially deleterious to biomolecules. This binding/unwinding process was mainly guided by a combination of van der Waals and π–π stacking interactions together with a continuous lateral migration of dsDNA. Moreover, the nanoscale dewetting also played an important role during the adsorption. These findings revealed the potential bio-effect of the C3N nanomaterial and its molecular mechanism, which might benefit the future applications of C3N-based nanostructures.

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“…C 3 N and BC 3 are two graphene derivatives with similar honeycomb structures. They have good application prospects in batteries, − gas separation, − drug transport agents, − biosensors, and so on. Because C 3 N and BC 3 are analogous in the structures, they are very easy to be integrated to construct heterostructures for novel nanodevices. − In our previous work, we found that C 3 N is more capable of attracting biomolecules (DNA and protein) to form stable binding , than BC 3 .…”

Section: Introductionmentioning

confidence: 99%

Electric Field-Controlled Peptide Self-Assembly through Funnel-Shaped Two-Dimensional Nanopores

Jia

Liu

et al. 2022

ACS Appl. Mater. Interfaces

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Self-assembly of biomolecules is critical for the realization of biological functions. Thus, the precise control of selfassembly has great significance in the design of biochips and biomedical agents. In this report, we design a Y-shaped funnel on a two-dimensional (2D) heterostructure, called 2D funnel, based on monolayered polyaniline carbon nitride (C 3 N) and boron carbide (BC 3 ), and study its application in the self-assembly state regulation of the peptide oligomer, using Aβ 16−21 as the representative model. Structurally, the 2D funnel is composed of three regions: channel area, triangle area, and barrier area. The channel and triangle areas show higher binding affinity to the peptide than that of the barrier area, which leads to the confinement of the peptide in the 2D funnel. Our results show that when an external electric field is applied along the 2D funnel, the oligomer is driven to migrate across the funnel. Its trajectory is confined inside the narrow channel area, which effectively causes peptide dissociation into the individual peptide chains. Then, when the external electric field is turned off, the separated peptide chains spontaneously assemble in the triangle area and tend to reunite. Our present findings propose a novel heterostructure platform, which enables the manipulation of the self-assembly state of peptides by switching the electric field, which could guide the design and fabrication of nanodevices for sensing and sequencing applications.

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Two-dimensional C₃N based sub-10 nanometer biosensor

Abstract: The current observation demonstrates the usefulness of the two-dimensional C3N system as a next generation bio-sensor for the sequencing of various nucleobases, offering new leads for future developments in bioelectronics, superior sensing architectures and sustainable designs.

Cited by 21 publications

References 47 publications

Partial Denaturation of Villin Headpiece upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet

Partial Denaturation of Villin Headpiece upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet

Potential Unwinding of Double-Stranded DNA upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet

Electric Field-Controlled Peptide Self-Assembly through Funnel-Shaped Two-Dimensional Nanopores

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Two-dimensional C3N based sub-10 nanometer biosensor

Abstract: The current observation demonstrates the usefulness of the two-dimensional C3N system as a next generation bio-sensor for the sequencing of various nucleobases, offering new leads for future developments in bioelectronics, superior sensing architectures and sustainable designs.

Cited by 21 publications

References 47 publications

Partial Denaturation of Villin Headpiece upon Binding to a Carbon Nitride Polyaniline (C3N) Nanosheet

Partial Denaturation of Villin Headpiece upon Binding to a Carbon Nitride Polyaniline (C3N) Nanosheet

Potential Unwinding of Double-Stranded DNA upon Binding to a Carbon Nitride Polyaniline (C3N) Nanosheet

Electric Field-Controlled Peptide Self-Assembly through Funnel-Shaped Two-Dimensional Nanopores

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Product

Resources

About

Two-dimensional C₃N based sub-10 nanometer biosensor

Partial Denaturation of Villin Headpiece upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet

Partial Denaturation of Villin Headpiece upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet

Potential Unwinding of Double-Stranded DNA upon Binding to a Carbon Nitride Polyaniline (C₃N) Nanosheet