Two Different Transcripts of a LAMMER Kinase Gene Play Opposite Roles in Disease Resistance

Duan, Lingling; Xiao, Wenfei; Xia, Fan; Liu, Hongbo; Xiao, Jinghua; Li, Xianghua; Wang, Shiping

doi:10.1104/pp.16.01245

Cited by 13 publications

(13 citation statements)

References 68 publications

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“…Temperature-induced AS can affect the processing of MIR400, providing evidence that AS plays a regulatory role in linking miRNAs with high temperature response (Yan et al, 2012). A recent study showed that OsDR11, a rice LAMMER kinase gene, can transcribe two AS transcripts, OsDR11L and OsDR11S, that play opposite roles in rice disease resistance (Duan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A global survey of alternative splicing in allopolyploid cotton: landscape, complexity and regulation

et al. 2017

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SummaryAlternative splicing (AS) is a crucial regulatory mechanism in eukaryotes, which acts by greatly increasing transcriptome diversity. The extent and complexity of AS has been revealed in model plants using high-throughput next-generation sequencing. However, this technique is less effective in accurately identifying transcript isoforms in polyploid species because of the high sequence similarity between coexisting subgenomes.Here we characterize AS in the polyploid species cotton. Using Pacific Biosciences singlemolecule long-read isoform sequencing (Iso-Seq), we developed an integrated pipeline for Iso-Seq transcriptome data analysis (https://github.com/Nextomics/pipeline-for-isoseq).We identified 176 849 full-length transcript isoforms from 44 968 gene models and updated gene annotation. These data led us to identify 15 102 fibre-specific AS events and estimate that c. 51.4% of homoeologous genes produce divergent isoforms in each subgenome. We reveal that AS allows differential regulation of the same gene by miRNAs at the isoform level. We also show that nucleosome occupancy and DNA methylation play a role in defining exons at the chromatin level.This study provides new insights into the complexity and regulation of AS, and will enhance our understanding of AS in polyploid species. Our methodology for Iso-Seq data analysis will be a useful reference for the study of AS in other species.

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Section: Introductionmentioning

confidence: 99%

A global survey of alternative splicing in allopolyploid cotton: landscape, complexity and regulation

et al. 2017

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“…Alternative splicing has been found to occur frequently in the primary transcripts of intron‐containing genes of eukaryotic organisms (Duan et al., ; Marquez et al., ), and can increase protein diversity, with different structures, functions or subcellular localization, and affect the stability of mRNA (Liu et al., ; Staiger and Brown, ). Alternative splicing is widely involved in development, particularly in plant defence responses.…”

Section: Introductionmentioning

confidence: 99%

“…oryzae is alternatively spliced, and the two forms of OsDR11 play opposite roles in the resistance pathway, with OsDR11L as a negative regulator and OsDR11S as a positive regulator. In addition, the transcription and kinase activity of OsDR11L is suppressed by OsDR11S (Duan et al., ). The tobacco N gene can generate two splice variants: before Tobacco mosaic virus (TMV) infection, the truncated transcript is prevalent, but, after TMV infection, the full‐length transcript becomes more prevalent.…”

Section: Introductionmentioning

confidence: 99%

A malectin‐like/leucine‐rich repeat receptor protein kinase gene, RLK‐V, regulates powdery mildew resistance in wheat

Liu

et al. 2018

Molecular Plant Pathology

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Pattern recognition receptors (PRRs) can trigger plant immunity through the recognition of pathogen-associated molecular patterns. In this study, we report that a malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, from Haynaldia villosa putatively acts as a PRR to positively regulate powdery mildew resistance caused by Blumeria graminis f. sp. tritici (Bgt) in wheat. RLK-V has two alternatively spliced transcripts corresponding to an intact RLK-V1.1 and a truncated RLK-V1.2 caused by intron retention. Expression analysis showed that both transcripts could be up-regulated by Bgt in resistant materials, whereas the functional RLK-V1.1 was expressed only after Bgt inoculation. Promoter activity assays indicated that RLK-V could respond to Bgt even in susceptible wheat. Silencing of RLK-V in Pm21-carrying resistant materials resulted in compromised resistance to Bgt. In addition, over-expression of RLK-V1.1 in Pm21-lacking susceptible Yangmai158 and SM-1 by single-cell transient expression and stable transformation in Yangmai158 could improve powdery mildew resistance. We propose that RLK-V regulates basal resistance to powdery mildew, which is also required for broad-spectrum resistance mediated by the Pm21 gene. Over-expression of RLK-V1.1 could trigger cell death in Nicotiana benthamiana, and RLK-V1.1 transgenic wheat accumulated more reactive oxygen species and displayed a stronger hypersensitive response than did the recipient, which led to enhanced Bgt resistance. However, constitutive activation of RLK-V1.1 resulted in the abnormal growth of transgenic plants.

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“…The motif is virtually almost 100% identical in higher eukaryotes such as animals and plants, but not in lower eukaryotes such as fungi (Kang et al, 2013). A phylogenic analysis of the LAMMER proteins from many organisms also formed four groups: animals (group I), plants (group II), fungi (group III), and slime mold (group IV) (Duan et al, 2016). These differences can be explained by the aspects of evolution; in higher eukaryotes, members of the LAMMER kinase subfamily have evolved into proteins with different and redundant roles, but lower eukaryotes members have evolved into proteins with multiple functions or loss-of-function variants (Bender and Fink, 1994; Kang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Heterologous expression of PK12 in Arabidopsis modulates the alternative splicing of mRNAs of specific developmental genes, and results in overall size reduction and prolonged life cycle (Savaldi-Goldstein et al, 2000; Savaldi-Goldstein, 2003). In rice, the two alternatively spliced transcripts of LAMMER kinase gene, OsDR11, function oppositely in the resistance against the rice pathogenic bacterium; Longer one functions negatively in disease resistance, which may suppress the Jasmonic Acid signaling, and shorter one may inhibit the function of longer one, leading to resistance against the bacterial pathogen, Xanthomonas oryzae (Duan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The Dual-Specificity LAMMER Kinase Affects Stress-Response and Morphological Plasticity in Fungi

Lim

Park

2019

Front. Cell. Infect. Microbiol.

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The morphological plasticity of fungal pathogens has long been implicated in their virulence and is often influenced by extracellular factors. Complex signal transduction cascades are critical for sensing stresses imposed by external cues such as antifungal drugs, and for mediating appropriate cellular responses. Many of these signal transduction cascades are well-conserved and involve in the distinct morphogenetic processes during the life cycle of the pathogenic fungi. The dual-specificity LAMMER kinases are evolutionarily conserved across species ranging from yeasts to mammals and have multiple functions in various physiological processes; however, their functions in fungi are relatively unknown. In this review, we first describe the involvement of LAMMER kinases in cell surface changes, which often accompany alterations in growth pattern and differentiation. Then, we focus on the LAMMER kinase-dependent molecular machinery responsible for the stress responses and cell cycle regulation. Last, we discuss the possible cross-talk between LAMMER kinases and other signaling cascades, which integrates exogenous and host signals together with genetic factors to affect the morphological plasticity and virulence in fungi.

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Two Different Transcripts of a LAMMER Kinase Gene Play Opposite Roles in Disease Resistance

Cited by 13 publications

References 68 publications

A global survey of alternative splicing in allopolyploid cotton: landscape, complexity and regulation

A global survey of alternative splicing in allopolyploid cotton: landscape, complexity and regulation

A malectin‐like/leucine‐rich repeat receptor protein kinase gene, RLK‐V, regulates powdery mildew resistance in wheat

The Dual-Specificity LAMMER Kinase Affects Stress-Response and Morphological Plasticity in Fungi

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