Two Different Species of
            <i>Mycoplasma</i>
            Endosymbionts Can Influence Trichomonas vaginalis Pathophysiology

Margarita, Valentina; Bailey, Nicholas P; Rappelli, Paola; Diaz, Nicia; Dessì, Daniele; Fettweis, Jennifer M.; Hirt, Robert P.; Fiori, Pier Luigi

doi:10.1128/mbio.00918-22

Cited by 16 publications

(31 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study reported that transcription of BspA-like proteins, surface proteins, and exosome proteins in T . vaginalis was modulated by Mycoplasma symbiosis [ 44 ], which implicates parasite pathogenicity. Therefore, we examined whether TH17 isolate contains Mycoplasma, showing that the Mycoplasma 16s rRNA gene was only amplified in the Mycoplasma-positive PM1 strain ( Fig 9A ) and Mycoplasma-like DNA puncta were only detected in the PM1 cytoplasm ( Fig 9B ), indicating that the TH17 isolate has no Mycoplasma symbiosis.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, Mycoplasma symbiosis was recently reported to correlate with the pathogenicity of T . vaginalis [ 44 ] and may increase parasite virulence. However, our results suggest that the symbiont is not the determinant conferring TH17 intrinsic cytoadherence.…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, histone acetylation may not serve as an appropriate marker for differentiating cytoadherence of T. vaginalis. In addition, Mycoplasma symbiosis was recently reported to correlate with the pathogenicity of T. vaginalis [44] and may increase parasite virulence. However, our results suggest that the symbiont is not the determinant conferring TH17 intrinsic cytoadherence.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

See 2 more Smart Citations

Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates

et al. 2023

View full text Add to dashboard Cite

Cytoadherence of Trichomonas vaginalis to human vaginal epithelial cells (hVECs) was previously shown to involve surface lipoglycans and several reputed adhesins on the parasite. Herein, we report some new observations on the host-parasite interactions of adherent versus nonadherent T. vaginalis isolates to hVECs. The binding of the TH17 adherent isolate to hVECs exhibited an initial discrete phase followed by an aggregation phase inhibited by lactose. T. vaginalis infection immediately induced surface expression of galectin-1 and -3, with extracellular amounts in the spent medium initially decreasing and then increasing thereafter over the next 60 min. Extracellular galectin-1 and -3 were detected on the parasite surface but only the TH17 adherent isolate could uptake galectin-3 via the lysosomes. Only the adherent isolate could morphologically transform from the round-up flagellate with numerous transient protrusions into a flat amoeboid form on contact with the solid surface. Cytochalasin D challenge revealed that actin organization was essential to parasite morphogenesis and cytoadherence. Real-time microscopy showed that parasite exploring and anchoring on hVECs via the axostyle may be required for initial cytoadherence. Together, the parasite cytoskeleton behaviors may collaborate with cell surface adhesion molecules for cytoadherence. The nonadherent isolate migrated faster than the adherent isolate, with motility transiently increasing in the presence of hVECs. Meanwhile, differential histone acetylation was detected between the two isolates. Also, TH17 without Mycoplasma symbiosis suggests that symbiont might not determine TH17 innate cytoadherence. Our findings regarding distinctive host-parasite interactions of the isolates may provide novel insights into T. vaginalis infection.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

See 1 more Smart Citation

Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It was also shown that the microbial association with M. hominis, as well as with Ca. M. girerdii, could modulate the virulence of TV [ 53 ]. Our recent results did not confirm this; however, we did not clear the TVs from M. hominis to test the same isolate with and without symbionts.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Trichomonas vaginalis Isolates Collected from Patients in Vienna between 2019 and 2021

Lesiak-Markowicz

Walochnik

Stary³

et al. 2022

IJMS

View full text Add to dashboard Cite

Trichomonas vaginalis (TV) is the causative agent of trichomoniasis, the most common nonviral sexually transmitted disease. TV can carry symbionts such as Trichomonas vaginalis virus (TVV) or Mycoplasma hominis. Four distinct strains of TV are known: TVV1, TVV2, TVV3, and TVV4. The aim of the current study was to characterise TV isolates from Austrian patients for the presence of symbionts, and to determine their effect on metronidazole susceptibility and cytotoxicity against HeLa cells. We collected 82 TV isolates and detected presence of TVV (TVV1, TVV2, or TVV3) in 29 of them (35%); no TVV4 was detected. M. hominis was detected in vaginal/urethral swabs by culture in 37% of the TV-positive patients; M. hominis DNA was found in 28% of the TV isolates by PCR. In 15% of the patients, M. hominis was detected in the clinical samples as well as within the respective TV isolates. In 22% of the patients, M. hominis was detected by culture only. In 11 patients, M. hominis was detected only within the respective cultured TV isolates (13%), while the swab samples were negative for M. hominis. Our results provide a first insight into the distribution of symbionts in TV isolates from Austrian patients. We did not observe significant effects of the symbionts on metronidazole susceptibility, cytotoxicity, or severity of symptoms.

show abstract

“…vaginalis was the only species in the reference data set representative of the Trichomonas genus, so it is possible that the particular species with increased gene expressed in macaques with ICD was not T. vaginalis'. Trichomonads do exhibit mucosal and host plasticity (Maritz et al, 2014). For example, T. vaginalis has been detected in the oral cavity (Costello et al, 2017) and respiratory tract (Duboucher et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Revisiting fecal metatranscriptomics analyses of macaques with idiopathic chronic diarrhoea with a focus on trichomonad parasites

Bailey

Hirt

2022

Parasitology

Self Cite

View full text Add to dashboard Cite

Trichomonads, anaerobic microbial eukaryotes members of the phylum Parabasalia, are common obligate extracellular symbionts that can lead to pathological or asymptomatic colonization of various mucosal surfaces in a wide range of animal hosts. Results from previous in vitro studies have suggested a number of intriguing mucosal colonization strategies by Trichomonads, notably highlighting the importance of interactions with bacteria. However, in vivo validation is currently lacking. A previous metatranscriptomics study into the cause of idiopathic chronic diarrhoea in macaques reported the presence of an unidentified protozoan parasite related to Trichomonas vaginalis. In this work, we performed a reanalysis of the published data in order to identify the parasite species present in the macaque gut. We also leveraged the information-rich metatranscriptomics data to investigate the parasite behaviour in vivo. Our results indicated the presence of at least 3 genera of Trichomonad parasite; Tetratrichomonas, Pentatrichomonas and Trichomitus, 2 of which had not been previously reported in the macaque gut. In addition, we identified common in vivo expression profiles shared amongst the Trichomonads. In agreement with previous findings for other Trichomonads, our results highlighted a relationship between Trichomonads and mucosal bacterial diversity which could be influential in health and disease.

show abstract

Two Different Species of Mycoplasma Endosymbionts Can Influence Trichomonas vaginalis Pathophysiology

Abstract: T. vaginalis and M. hominis form a unique case of endosymbiosis that modulates the parasite’s pathobiology. Recently, a new nonculturable mycoplasma species (“ Candidatus Mycoplasma girerdii”) has been described as closely associated with the protozoon.

Cited by 16 publications

References 100 publications

Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates

Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates

Characterisation of Trichomonas vaginalis Isolates Collected from Patients in Vienna between 2019 and 2021

Revisiting fecal metatranscriptomics analyses of macaques with idiopathic chronic diarrhoea with a focus on trichomonad parasites

Contact Info

Product

Resources

About