Two detection methods of genetically modified maize and the state of its import into Japan

Yamaguchi, Hideaki; Sasaki, Kazuo; Umetsu, Hironori; Kamada, Hiroshi

doi:10.1016/s0956-7135(02)00062-2

Cited by 29 publications

(20 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The PCR technique is very specific, sensitive and safe and is able to detect both events of genetic modification (Bertheau et al, 2002;Giovannini and Concillo, 2002) and distinguish events associated with different gene constructs expressing the same protein (Yamaguchi et al, 2003). However, this technique also presents some limitations, such as: 1) the difficulty involved in designing primers, as it is necessary to know the genetic sequence of the DNA introduced into the GMO, and this information is usually confidential (Holst-Jensen et al, 2003); 2) the need for appropriate equipment and trained personnel; 3) the relatively high cost because the test is specific for each genetic alteration introduced; and 4) the special care required to avoid sample contamination (Miraglia et al, 2004, Yamaguchi et al, 2003.…”

Section: Pcrmentioning

confidence: 99%

“…However, this technique also presents some limitations, such as: 1) the difficulty involved in designing primers, as it is necessary to know the genetic sequence of the DNA introduced into the GMO, and this information is usually confidential (Holst-Jensen et al, 2003); 2) the need for appropriate equipment and trained personnel; 3) the relatively high cost because the test is specific for each genetic alteration introduced; and 4) the special care required to avoid sample contamination (Miraglia et al, 2004, Yamaguchi et al, 2003.…”

Section: Pcrmentioning

confidence: 99%

See 1 more Smart Citation

Biosafety and Detection of Genetically Modified Organisms

Ferreira¹,

Cançado²,

Borém³

et al. 2012

Transgenic Plants - Advances and Limitations

View full text Add to dashboard Cite

Section: Pcrmentioning

confidence: 99%

Section: Pcrmentioning

confidence: 99%

Biosafety and Detection of Genetically Modified Organisms

Ferreira¹,

Cançado²,

Borém³

et al. 2012

Transgenic Plants - Advances and Limitations

View full text Add to dashboard Cite

“…It was found that the PCR is suitable for analyzing food Matsuoka et al, 2002;Meyer et al, 1996;Allmann et al, 1993) as it is the method selected for detecting GMOs in food (Yamaguchi et al, 2003;Anklam et al, 2002;Lipp et al, 2000;Meyer, 1999;Vollenhofer et al, 1999). This method includes a first amplification of certain soy sequences from plant DNA.…”

Section: Introductionmentioning

confidence: 99%

PCR Based Detection of Genetically Modified Soy in Processed Foods Commercially Available in Saudi Arabia

Alaraidh

2009

SQU Journal for Science

View full text Add to dashboard Cite

In this research, PCR (polymerase chain reaction) technique was applied to detect the presence of GMO sold in the Saudi Arabian market. This method was applied to detect genetically modified soy (GM-soy) in particular the roundup ready soy (RRS). To confirm the presence of soy, samples were first tested for the existence of the soy specific lectin gene. A total of eighty samples were tested out of which two samples tested positive as GM-soy. Not surprisingly, the findings showed the existence of GM-soy in food products in Saudi. This supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops.

show abstract

“…It is also the only method that can be applied to field samples. A qualitative and quantitative screening PCR-based method has been proposed by food researchers for specific detection of GM soybean and maize (Jankiewicz et al, 1999;Yamaguchi et al, 2003;Yun et al, 2004).…”

mentioning

confidence: 99%

Monitoring the occurrence of genetically modified soybean and maize around cultivated fields and at a grain receiving port in Korea

Kim

Yeon

et al. 2006

J. Plant Biol.

View full text Add to dashboard Cite

Increased imports of genetically modified (GM) soybean and maize might cause genetic contamination of those crops that are conventionally bred, as well as wild soybeans within Korea. Leaves of maize and both cultivated and wild soybeans were sampled in and near rural fields to detect the presence of transgenes. Roadsides around a major grain port in Incheon were also surveyed to monitor the occurrence of incoming GM soybean and maize. The amplificabUity of DNA extracted from the collected samples was determined by PCR using soybean-or maize-specific primers: lectin and zein genes, respectively. The presence or absence of transgenes was detected by primer sets for the 35S and nos genes. Transgenes were not found in the cultivated or wild soybean or in the maize collected from cultivated fields. However, we obtained one GM maize plant among seven along the roadsides around Incheon Port. Although the effect of a single GM maize plant would be negligible and would not pose any threat to natural environments, an increase in the import of GM plants might lead to future, unapproved cultivation of GM crops. Therefore, appropriate monitoring is necessary to detect the occurrence of GM plants in areas around grain receiving ports and within agroecosystems.

show abstract

Two detection methods of genetically modified maize and the state of its import into Japan

Cited by 29 publications

References 17 publications

Biosafety and Detection of Genetically Modified Organisms

Biosafety and Detection of Genetically Modified Organisms

PCR Based Detection of Genetically Modified Soy in Processed Foods Commercially Available in Saudi Arabia

Monitoring the occurrence of genetically modified soybean and maize around cultivated fields and at a grain receiving port in Korea

Contact Info

Product

Resources

About