Two decades of blaVIM-2-producing Pseudomonas aeruginosa dissemination: an interplay between mobile genetic elements and successful clones

Botelho, João; Grosso, Filipa; Quinteira, Sandra; Brilhante, Michael; Ramos, Helena; Peixe, Luı́sa

doi:10.1093/jac/dkx517

Cited by 37 publications

(22 citation statements)

References 50 publications

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“…One C. freundii (Cfr-12, ST129) carried the bla VIM-2 gene in a 30 kb plasmid. It had almost 100% identity with pJB12 (KX889311), detected in four P. aeruginosa from inpatients of two different hospitals in Portugal 20 , 21 . pJB12 has the bla VIM-2 -carrying In58 integron immersed in Tn 6352 , a transposon composed of the In58 and IS Pa17 elements.…”

Section: Resultsmentioning

confidence: 99%

Dissemination of Verona Integron-encoded Metallo-β-lactamase among clinical and environmental Enterobacteriaceae isolates in Ontario, Canada

Köhler

Tijet

Kim

et al. 2020

Sci Rep

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Surveillance data from Southern Ontario show that a majority of Verona Integron-encoded Metallo-β-lactamase (VIM)-producing Enterobacteriaceae are locally acquired. To better understand the local epidemiology, we analysed clinical and environmental blaVIM-positive Enterobacteriaceae from the area. Clinical samples were collected within the Toronto Invasive Bacterial Diseases Network (2010–2016); environmental water samples were collected in 2015. We gathered patient information on place of residence and hospital admissions prior to the diagnosis. Patients with and without plausible source of acquisition were compared regarding risk exposures. Microbiological isolates underwent whole-genome sequencing (WGS); blaVIM carrying plasmids were characterized. We identified 15 patients, thereof 11 with blaVIM-1-positive Enterobacter hormaechei within two genetic clusters based on WGS. Whereas no obvious epidemiologic link was identified among cluster I patients, those in cluster II were connected to a hospital outbreak. Except for patients with probable acquisition abroad, we did not identify any further risk exposures. Two blaVIM-1-positive E. hormaechei from environmental waters matched with the clinical clusters; plasmid sequencing suggested a common ancestor plasmid for the two clusters. These data show that both clonal spread and horizontal gene transfer are drivers of the dissemination of blaVIM-1-carrying Enterobacter hormaechei in hospitals and the aquatic environment in Southern Ontario, Canada.

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Section: Resultsmentioning

confidence: 99%

Dissemination of Verona Integron-encoded Metallo-β-lactamase among clinical and environmental Enterobacteriaceae isolates in Ontario, Canada

Köhler

Tijet

Kim

et al. 2020

Sci Rep

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“…The aacA4 gene cassette has a C residue at nucleotide position 329, encoding a serine associated with gentamicin resistance [51]. The same cassette array has been observed previously among isolates from Portuguese hospitals [22]. The integron is of the In4 type, with a complete 5’-CS bounded by the 25 bp inverted repeat IRi, 2,239 bp of the 3’-CS and IS 6100 flanked by two fragments of the IRt end of Tn 402 [9, 52].…”

Section: Resultsmentioning

confidence: 72%

Megaplasmids on the Rise: Combining Sequencing Approaches to Fully Resolve a Carbapenemase-Encoding Plasmid in a Proposed NovelPseudomonasSpecies

Botelho

Lood

Partridge

et al. 2019

Preprint

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27Horizontal transfer of plasmids plays a pivotal role in the dissemination of antibiotic 28 resistance genes and emergence of multidrug-resistant bacteria. Sequencing of plasmids 29 is thus paramount for the success of accurate epidemiological tracking strategies in the 30 hospital setting and routine surveillance. Here, we combine Nanopore and Illumina 31 sequencing to fully assemble a carbapenemase-encoding megaplasmid carried by a 32 clinical isolate belonging to a putative novel Pseudomonas species. FFUP_PS_41 has a 33 multidrug resistance phenotype and was initially identified as Pseudomonas putida, but 34 an average nucleotide identity below the cut-off for species delineation suggests a new 35 species related to the P. putida phylogenetic group. FFUP_PS_41 harbors a 498,516-bp 36 untypable megaplasmid (pJBCL41) with low similarity compared with publicly 37 available plasmids. pJBCL41 contains a full set of genes for self-transmission and genes 38 predicted to be responsible for plasmid replication, partitioning, maintenance and heavy 39 metal resistance. pJBCL41 carries a class 1 integron with the |aacA7|bla VIM-2 |aacA4| 40 cassette array (In103) located within a defective Tn402-like transposon that forms part 41 of a 50,273-bp mosaic region bound by 38-bp inverted repeats typical of the Tn3 family 42 and flanked by 5-bp direct repeats. This region is composed of different elements, 43 including additional transposon fragments, five insertion sequences and a Tn3-Derived 44Inverted-Repeat Miniature Element. The hybrid Nanopore/Illumina approach resulted in 45 contiguous assemblies and allowed us to fully resolve a carbapenemase-encoding 46 megaplasmid from Pseudomonas spp. The identification of novel megaplasmids will 47 shed a new light on the evolutionary effects of gene transfer and the selective forces 48 driving AR. 49

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“…Interestingly, we also identified two wastewater isolates that belong to ST815, which appear to be uncommon but are resistant to four major antipseudomonal drugs, including carbapenems. The only record of ST815 comes from a study in Portugal [ 71 ]. These isolates also carried the ciprofloxacin-modifying crpP resistance gene (50, 51), which has been shown to marginally increase MICs to FQs through ciprofloxacin phosphorylation [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Efflux Pump-Driven Antibiotic and Biocide Cross-Resistance in Pseudomonas aeruginosa Isolated from Different Ecological Niches: A Case Study in the Development of Multidrug Resistance in Environmental Hotspots

et al. 2020

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Pseudomonas aeruginosa is an opportunistic pathogen displaying high intrinsic antimicrobial resistance and the ability to thrive in different ecological environments. In this study, the ability of P. aeruginosa to develop simultaneous resistance to multiple antibiotics and disinfectants in different natural niches were investigated using strains collected from clinical samples, veterinary samples, and wastewater. The correlation between biocide and antimicrobial resistance was determined by employing principal component analysis. Molecular mechanisms linking biocide and antimicrobial resistance were interrogated by determining gene expression using RT-qPCR and identifying a potential genetic determinant for co- and cross-resistance using whole-genome sequencing. A subpopulation of P. aeruginosa isolates belonging to three sequence types was resistant against the common preservative benzalkonium chloride and showed cross-resistance to fluoroquinolones, cephalosporins, aminoglycosides, and multidrug resistance. Of these, the epidemiological high-risk ST235 clone was the most abundant. The overexpression of the MexAB-OprM drug efflux pump resulting from amino acid mutations in regulators MexR, NalC, or NalD was the major contributing factor for cross-resistance that could be reversed by an efflux pump inhibitor. This is the first comparison of antibiotic-biocide cross-resistance in samples isolated from different ecological niches and serves as a confirmation of laboratory-based studies on biocide adapted isolates. The isolates from wastewater had a higher incidence of multidrug resistance and biocide-antibiotic cross-resistance than those from clinical and veterinary settings.

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Two decades of blaVIM-2-producing Pseudomonas aeruginosa dissemination: an interplay between mobile genetic elements and successful clones

Abstract: The dissemination of blaVIM-2 carrying integrons is associated with a complex interplay between different mobile genetic elements, including the overlooked integrative conjugative elements, and successful spread of particular clones.

Cited by 37 publications

References 50 publications

Dissemination of Verona Integron-encoded Metallo-β-lactamase among clinical and environmental Enterobacteriaceae isolates in Ontario, Canada

Dissemination of Verona Integron-encoded Metallo-β-lactamase among clinical and environmental Enterobacteriaceae isolates in Ontario, Canada

Megaplasmids on the Rise: Combining Sequencing Approaches to Fully Resolve a Carbapenemase-Encoding Plasmid in a Proposed NovelPseudomonasSpecies

Efflux Pump-Driven Antibiotic and Biocide Cross-Resistance in Pseudomonas aeruginosa Isolated from Different Ecological Niches: A Case Study in the Development of Multidrug Resistance in Environmental Hotspots

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