Two consecutive randomized controlled pertussis booster trials in children initially vaccinated in infancy with an acellular vaccine: The first with a five-component Tdap vaccine to 5-year olds and the second with five- or monocomponent Tdap vaccines at age 14–15 years

Carlsson, Rose-Marie; Gustafsson, Lennart; Hallander, Hans O.; Ljungman, Margaretha; Olin, Patrick; Gothefors, Leif; Nilsson, Lennart; Netterlid, Eva

doi:10.1016/j.vaccine.2015.05.079

Cited by 7 publications

(3 citation statements)

References 37 publications

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“…Peripheral venous blood was collected in BD Vacutainer CPT tubes at the day prior to vaccination and at days 4, 14, and 28, and months 6 and 12 after vaccination, followed by PBMCs isolation and serum collection. The Tdap vaccine study is a randomized, multicenter study in which the immunogenicity and safety of the tetanus, diphtheria, and acellular pertussis vaccine booster were evaluated (72,73). In part of this clinical trial, adolescents (14-15 years of age) with a vaccination history of 3 primary immunizations of DTaP5 (Connaught HCP4DT) at 3, 5, and 12 months of age plus a booster immunization of Tdap5 (Triaxis, Sanofi Pasteur) at 5.5 years of age received another booster dose of Tdap5 (Triaxis, Sanofi Pasteur).…”

Section: Methodsmentioning

confidence: 99%

Live attenuated pertussis vaccine BPZE1 induces a broad antibody response in humans

Lin¹,

Apostolović²,

Jahnmatz³

et al. 2020

Journal of Clinical Investigation

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BACKGROUND. The live attenuated BPZE1 vaccine candidate induces protection against B. pertussis and prevents nasal colonization in animal models. Here we report on the responses in humans receiving a single intranasal administration of BPZE1. METHODS. We performed multiple assays to dissect the immune responses induced in humans (n = 12) receiving BPZE1, with particular emphasis on the magnitude and characteristics of the antibody responses. Such responses were benchmarked to adolescents (n = 12) receiving the complete vaccination program of the currently used acellular pertussis vaccine (aPV). Using immunoproteomics analysis, potentially novel immunogenic B. pertussis antigens were identified. RESULTS. All BPZE1 vaccinees showed robust B. pertussis-specific antibody responses with regard to significant increase in 1 or more of the following parameters: IgG, IgA, and memory B cells to B. pertussis antigens. BPZE1-specific T cells showed a Th1 phenotype, and the IgG exclusively consisted of IgG1 and IgG3. In contrast, all aPV vaccines showed a Th2-biased response. Immunoproteomics profiling revealed that BPZE1 elicited broader and different antibody specificities to B. pertussis antigens as compared with the aPV that primarily induced antibodies to the vaccine antigens. Moreover, BPZE1 was superior at inducing opsonizing antibodies that stimulated ROS production in neutrophils and enhanced bactericidal function, which was in line with the finding that antibodies against adenylate cyclase toxin were only elicited by BPZE1. CONCLUSION. The breadth of the antibodies, the Th1-type cellular response, and killing mechanisms elicited by BPZE1 may hold prospects of improving vaccine efficacy and protection against B. pertussis transmission.

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Section: Methodsmentioning

confidence: 99%

Live attenuated pertussis vaccine BPZE1 induces a broad antibody response in humans

Lin¹,

Apostolović²,

Jahnmatz³

et al. 2020

Journal of Clinical Investigation

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“…The Danish aPV is without remnants of other proteins from B. pertussis (34). If the aPV groups have had previous contact with other B. pertussis antigens than PTx, it is therefore, not originating from the vaccine.…”

Section: Discussionmentioning

confidence: 99%

Antibody Specificity Following a Recent Bordetella pertussis Infection in Adolescence Is Correlated With the Pertussis Vaccine Received in Childhood

et al. 2019

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Bordetella (B.) pertussis resurgence affects not only the unvaccinated, but also the vaccinated population. Different vaccines are available, however, it is currently unknown whether the type of childhood vaccination has an influence on antibody responses following a B. pertussis infection later in life. Therefore, the study aim was to profile serum antibody responses in young adults with suspected B. pertussis infections, immunized during childhood with either whole-cell (wPV) or monocomponent acellular pertussis (aPV) vaccines. Serum anti-pertussis toxin (PTx) IgG antibody levels served as an indicator for a recent B. pertussis infection. Leftover sera from a diagnostic laboratory from 36 Danish individuals were included and divided into four groups based on immunization background (aPV vs . wPV) and serum anti-PTx IgG levels (– vs . +). Pertussis-specific IgG/IgA antibody levels and antigen specificity were determined by using multiplex immunoassays (MIA), one- and two-dimensional immunoblotting (1 & 2DEWB), and mass spectrometry. Besides enhanced anti-PTx levels, wPV(+) and aPV(+) groups showed increased IgG and IgA levels against pertactin, filamentous hemagglutinin, fimbriae 2/3, and pertussis outer membrane vesicles (OMV). In the wPV(–) and aPV(–) groups, only low levels of anti-OMV antibodies were detected. 1DEWB demonstrated that antibody patterns differed between groups but also between individuals with the same immunization background and anti-PTx levels. 2DWB analysis for serum IgG revealed 133 immunogenic antigens of which 40 were significantly different between groups allowing to differentiate wPV(+) and aPV(+) groups. Similarly, for serum IgA, 7 of 47 immunogenic protein spots were significantly different. This study demonstrated that B. pertussis infection-induced antibody responses were distinct on antigen level between individuals with either wPV or aPV immunization background. Importantly, only 2DEWB and not MIA could detect these differences indicating the potential of this method. Moreover, in individuals immunized with an aPV containing only PTx in childhood, the infection-induced antibody responses were not limited to PTx alone.

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“…Detoxification by hydrogen peroxide has been shown to result in a lower degree of epitope impairment of the toxin compared with formaldehyde, and the generated immune response may therefore be more effective [ 12 , 13 ]. Moreover, the Danish aP vaccines elicit a stronger antibody response against pertussis toxin than a number of other aPs [ 11 , 14 , 15 ].…”

Section: Background On Pertussis Vaccination and Diagnostics In Denmamentioning

confidence: 99%

Epidemiology of pertussis in Denmark, 1995 to 2013

2016

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We describe incidence and age distribution of laboratory-confirmed pertussis in Denmark from 1995 to 2013. Notification has been mandatory since 2007. Since 1997, an acellular monocomponent vaccine has been used. The latest epidemic occurred in 2002 with an incidence of 36 per 100,000; since 1995, only six infant deaths have been recorded. The inter-epidemic incidence lies below 10 per 100,000. In 1995, the mean age of confirmed cases was 9.2 years (95% confidence interval (CI): 7.9–10.5; median: 5.1), this gradually increased to 23.9 years in 2013 (95% CI: 22.0–25.8; median: 15.7). In 1995, 14% of laboratory-confirmed cases were 20 years and older, 43% in 2013. In the study period, the highest incidence among children was among those younger than one year with incidences between 84 and 331 per 100,000 in inter-epidemic periods (mean: 161/100,000) and 435 for the epidemic in 2002. After introduction of a preschool booster in 2003, the highest incidence among children one year and older changed gradually from three to five-year-olds in 2003 to 12 to 14-year-olds in 2013. In 2013, PCR was the primary method used for laboratory-diagnosis of pertussis in Denmark, while serology was the method with the highest percentage of positive results.

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Two consecutive randomized controlled pertussis booster trials in children initially vaccinated in infancy with an acellular vaccine: The first with a five-component Tdap vaccine to 5-year olds and the second with five- or monocomponent Tdap vaccines at age 14–15 years

Cited by 7 publications

References 37 publications

Live attenuated pertussis vaccine BPZE1 induces a broad antibody response in humans

Live attenuated pertussis vaccine BPZE1 induces a broad antibody response in humans

Antibody Specificity Following a Recent Bordetella pertussis Infection in Adolescence Is Correlated With the Pertussis Vaccine Received in Childhood

Epidemiology of pertussis in Denmark, 1995 to 2013

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