Two confirmatory tests for identification of Neisseria gonorrhoeae from primary culture

Ridderhof, John C.; Vaughan, Matthew; Tinney, A.; Meier, Frederick A.; Dalton, Harry P.

doi:10.1128/jcm.28.3.619-620.1990

J Clin Microbiol

1990

DOI: 10.1128/jcm.28.3.619-620.1990

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Two confirmatory tests for identification of Neisseria gonorrhoeae from primary culture

John C. Ridderhof

Matthew Vaughan

A. Tinney

et al.

Abstract: We compared a fluorescent monoclonal antibody and a DNA probe for identification of Neisseria gonorrhoeae (GC) from primary genital cultures of presumptive GC and selected bacterial isolates other than GC. The monoclonal antibody was sensitive (94%) and specific (100%) enough to identify GC in selective primary genital culture. The DNA probe was sensitive (95%) but not adequately specific (65%) to function as a confirmatory test.

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“…Strains of N. gonorrhoeae that resulted in false-negative DFA results have often (1,8,22,23), but not always (1,13), been reported to be ␤-lactamase positive. The reported specificities of GonoGen, MicroTrak DFA, and CTA carbohydrates were 99.1 to 100% (3,5,11,20), 100% (2,3,8,10,19,25), and 80.8 to 100% (3, 15), respectively. Maltosenegative strains of N. meningitidis exist and therefore could be misidentified as N. gonorrhoeae if a carbohydrate utilization assay were used to identify the isolates (6,7,13,15,21,27).…”

mentioning

confidence: 99%

Comparison of GonoGen, GonoGen II, and MicroTrak direct fluorescent-antibody test with carbohydrate fermentation for confirmation of culture isolates of Neisseria gonorrhoeae

Kellogg

Orwig

1995

J Clin Microbiol

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When testing 248 clinical isolates of Neisseria gonorrhoeae, the sensitivity was 100% with GonoGen (Becton Dickinson Microbiology Systems), 99.6% (247 of 248) with GonoGen II (Becton Dickinson), 97.2% (241 of 248) with the MicroTrak direct fluorescent-antibody test (Syva), and 97.6% (242 of 248) with Rapid Fermentation Agar carbohydrates (Remel). Of 62 isolates of other Neisseria species, none was misidentified as N. gonorrhoeae by GonoGen, MicroTrak, or Rapid Fermentation Agar carbohydrates but 7 (31.8%) of 22 isolates of N. meningitidis gave strong, repeatedly false-positive results with GonoGen II. The sensitivity of all four assays was good to excellent, but all positive GonoGen II results should be confirmed with an independent assay, especially when isolates are recovered from sites where N. meningitidis is likely. Positive results from any of the assays should be routinely confirmed when dictated by specific clinical, legal, or microbiological circumstances.

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mentioning

confidence: 99%

Comparison of GonoGen, GonoGen II, and MicroTrak direct fluorescent-antibody test with carbohydrate fermentation for confirmation of culture isolates of Neisseria gonorrhoeae

Kellogg

Orwig

1995

J Clin Microbiol

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Comparison of monoclonal antibody methods and a ribosomal ribonucleic acid probe test forNeisseria gonorrhoeae culture confirmation

Janda

Wilcoski²,

Mandel³

et al. 1993

Eur. J. Clin. Microbiol. Infect. Dis.

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Recently, a chemiluminescent nucleic acid probe test that specifically detects the ribosomal ribonucleic acid of Neisseria gonorrhoeae has been released for clinical laboratory use (AccuProbe Neisseria gonorrhoeae). In this study, three coagglutination tests (GonoGen I, Meritec GC, and GC Omni), the GonoGen II immunofiltration method and the Micro Trak Neisseria gonorrhoeae fluorescent monoclonal antibody test were compared with AccuProbe for identification of gonococci. Strains tested (n = 376) included 194 Neisseria gonorrhoeae, 82 Neisseria meningitidis, 32 Neisseria lactamica, 32 Neisseria species, 32 Moraxella catarrhalis, 2 Moraxella spp. and 2 Kingella denitrificans. The GonoGen I, Meritec GC and GC Omni coagglutination tests produced clearly positive results for 93.8%, 92.3% and 95.9% of the gonococci, respectively. The GonoGen II unequivocally identified 91.8% and the MicroTrak fluorescent antibody test identified 90.7% with 2+ or greater fluorescence. AccuProbe identified 100% of the gonococci tested. GonoGen I and GonoGen II were 98% specific, Meritec GC was 99% specific and the specificity of the GC Omni, MicroTrak fluorescent antibody and AccuProbe tests was 100%. While antibody-based tests were reliable when results were clearly interpretable, the AccuProbe was the only confirmatory test that was 100% accurate. Serotyping studies indicate that an array of beta-lactamase positive and negative gonococcal serotypes fail to react with the monoclonal antibody-based tests in general and with the fluorescent antibody test in particular.

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Novel Genus-Specific PCR-Based Assays for Rapid Identification of Neisseria Species and Neisseria meningitidis

Lansac

Picard²,

Ménard³

et al. 2000

European Journal of Clinical Microbiology & Infectious Dise

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This study presents the development of polymerase chain reaction (PCR)-based tests for the identification and detection of Neisseria species and Neisseria meningitidis. Currently, isolating and identifying these pathogens using conventional biochemical methods require 48-72 h. To improve speed and accuracy in diagnosing Neisseria infections, simple PCR-based tests that are specific for the genus Neisseria and the species Neisseria meningitidis have been developed. The genus-specific and species-specific DNA sequences were chosen by selecting and analyzing available database sequences. Neisseria-specific and Neisseria meningitidis-specific primer pairs were derived from the genes asd (coding for the aspartate beta-semialdehyde dehydrogenase) and ctrA (coding for a conserved outer membrane protein), respectively. Both the Neisseria-specific and Neisseria meningitidis-specific PCR assays were specific (they amplified only DNA from the target genus or species, out of 84 bacterial species tested). In addition, the Neisseria-specific assay amplified DNA from 321 of 322 strains tested representing 13 species of Neisseria, while the Neisseria meningitidis-specific assay amplified DNA from all 256 strains tested representing nine serogroups of Neisseria meningitidis. These PCR assays, which can be combined in multiplex, have been adapted to ensure that they are simple and can be performed within approximately 90 min. The tests provide new diagnostic tools for identifying Neisseria infections.

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Two confirmatory tests for identification of Neisseria gonorrhoeae from primary culture

Cited by 4 publications

References 4 publications

Comparison of GonoGen, GonoGen II, and MicroTrak direct fluorescent-antibody test with carbohydrate fermentation for confirmation of culture isolates of Neisseria gonorrhoeae

Comparison of GonoGen, GonoGen II, and MicroTrak direct fluorescent-antibody test with carbohydrate fermentation for confirmation of culture isolates of Neisseria gonorrhoeae

Comparison of monoclonal antibody methods and a ribosomal ribonucleic acid probe test forNeisseria gonorrhoeae culture confirmation

Novel Genus-Specific PCR-Based Assays for Rapid Identification of Neisseria Species and Neisseria meningitidis

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