Two-component signaling pathways modulate drug resistance of

Wu, Shizhou; Lin, Kaifeng; Liu, Yunjie; Zhang, Hui; Lei, Lei

doi:10.3892/br.2020.1312

Cited by 23 publications

(23 citation statements)

References 48 publications

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“…In a previous study, we demonstrated that the essential YycFG TCS was closely related to biofilm formation and extracellular matrix organization [ 16 ]. However, since the YycFG TCS is essential to S. aureus viability, the construction of deletion mutants of YycFG was unsuccessful [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Antisense yycG modulates the susceptibility of Staphylococcus aureus to hydrogen peroxide via the sarA

Liu

Lei

et al. 2021

BMC Microbiol

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Background The infectious pathogen Staphylococcus aureus (S. aureus) is primarily associated with osteomyelitis. Hydrogen peroxide drainage is an effective antimicrobial treatment that has been adopted to combat S. aureus infections. Previous investigations have indicated that the antisense RNA (asRNA) strategy negatively modulates S. aureus YycFG TCS, and it significantly disrupts biofilm formation. However, the effects of the antisense yycG RNA (ASyycG) strategy on the susceptibility of biofilm-producing S. aureus to hydrogen peroxide and the mechanisms underlying this effect have not been elucidated to date. Results Overexpression of ASyycG inhibited the transcription of biofilm formation-related genes, including sarA and icaA. Additionally, the CFU counts and the live bacterial ratios of ASyycG biofilm-producing S. aureus treated with H2O2 were notably reduced across the groups. Notably, the predicted promoter regions of the sarA and icaA genes were directly regulated by YycF. Conclusions ASyycG was observed to sensitize biofilm-producing S. aureus to H2O2 intervention synergistically via the sarA and thus may represent a supplementary strategy for managing osteomyelitis. However, future in-depth studies should attempt to replicate our findings in animal models, such as the rat osteomyelitis model.

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Section: Introductionmentioning

confidence: 99%

Antisense yycG modulates the susceptibility of Staphylococcus aureus to hydrogen peroxide via the sarA

Liu

Lei

et al. 2021

BMC Microbiol

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“…TCS is one of the dominating pathways by which bacteria adapt to the external environment, and it plays an important role in regulating the formation of bacterial biofilms, the expression of virulence genes, the synthesis of the cell wall, and bacterial activity [ 30 ]. Generally, multiple mechanisms exist in the TCS, such as cross-regulation, integration, and coordination of various input stimuli to control biofilm formation [ 31 ]. Sucrose, a substrate for the synthesis of extracellular polysaccharides but not oligosaccharides, is beneficial for biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Understanding the Interactions between Staphylococcus aureus and the Raw-Meat-Processing Environment Isolate Klebsiella oxytoca in Dual-Species Biofilms via Discovering an Altered Metabolic Profile

Chen

Tian

et al. 2021

Microorganisms

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In a raw-meat-processing environment, members of the Enterobacteriaceae family can coexist with Staphylococcus aureus to form dual-species biofilms, leading to a higher risk of food contamination. However, very little is known about the effect of inter-species interactions on dual-species biofilm formation. The aim of this study was to investigate the interactions between S. aureus and raw-meat-processing environment isolates of Klebsiella oxytoca in dual-species biofilms, by employing an untargeted metabolomics tool. Crystal violet staining assay showed that the biomass of the dual-species biofilm significantly increased and reached its maximum after incubation for 21 h, compared with that of single species grown alone. The number of K. oxytoca in the dual-species biofilm was significantly higher than that of S. aureus. Field emission scanning electron microscopy (FESEM) revealed that both species were evenly distributed, and were tightly wrapped by extracellular polymeric substances in the dual-species biofilms. Ultra-high-pressure liquid chromatography equipped with a quadrupole-time-of-flight mass spectrometer (UHPLC-Q-TOF MS) analysis exhibited a total of 8184 positive ions, and 6294 negative ions were obtained from all test samples. Multivariate data analysis further described altered metabolic profiling between mono- and dual-species biofilms. Further, 18 and 21 different metabolites in the dual-species biofilm were screened as biomarkers by comparing the mono-species biofilms of S. aureus and K. oxytoca, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways that were exclusively upregulated in the dual-species biofilm included ABC transporters, amino acid metabolism, and the two-component signal transduction system. Our results contribute to a better understanding of the interactive behavior of inter-species biofilm communities, by discovering altered metabolic profiling.

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“…Two-component regulatory systems (TCSs) exist ubiquitously in bacteria to adapt to stimuli and nutrition alterations from the external environment [11,12]. Typically, the TCS contains a histidine kinase (HK), which can autophosphorylate a conserved histidine residue in response to extracellular stimulus, and a response regulator (RR), which can transfer the phosphoryl group from HK [13].…”

Section: Introductionmentioning

confidence: 99%

An Antisense yycF RNA Modulates Biofilm Organization of Methicillin-Resistant Staphylococcus aureus and Pathogenicity in a Rat Model of Osteomyelitis

Liu

Lei

et al. 2021

Antibiotics

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Staphylococcus aureus (S. aureus) is one of most common opportunistic pathogens and is attributed to several human infections. The increasing incidence of methicillin-resistant S. aureus (MRSA) is a serious clinical threat for osteomyelitis crisis. The YycFG two-component system of S. aureus regulates genes associated with biofilm formation. To investigate the potential role of an antisense yycF RNA in the regulation of transcription levels of yycF and associated effects on biofilm formation and pathogenicity, antisense yycF (ASyycF) RNA was detected by RT-PCR and 5′ RACE assays. ASyycF overexpression mutants were constructed, and the biofilm biomass was determined by crystal violet microtiter assay and scanning electron microscopy (SEM). Quantitative RT-PCR and Western blotting analyses were used to detect whether ASyycF overexpression inhibited the transcription and translation of biofilm-related genes. Then, a rat tibial infective model was used to evaluate the pathogenicity of ASyycF overexpression in vivo. ASyycF transcription led to reductions in YycF production and biofilm formation. Overexpression of ASyycF inhibited the transcription and translation of biofilm-related genes. The sensitivity to vancomycin was improved in ASyycF-overexpressing MRSA. Furthermore, ASyycF inhibited MRSA invasion in a rat tibial infection model. From this study, the expression of the YycF protein was found to be inversely correlated with different levels of ASyycF transcription. The biofilm biomass and pathogenicity decreased in the ASyycF-overexpressing mutant. Thus, the current evidence may support ASyycF as a supplementary strategy for managing S. aureus and MRSA infections.

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Two-component signaling pathways modulate drug resistance of

Cited by 23 publications

References 48 publications

Antisense yycG modulates the susceptibility of Staphylococcus aureus to hydrogen peroxide via the sarA

Antisense yycG modulates the susceptibility of Staphylococcus aureus to hydrogen peroxide via the sarA

Understanding the Interactions between Staphylococcus aureus and the Raw-Meat-Processing Environment Isolate Klebsiella oxytoca in Dual-Species Biofilms via Discovering an Altered Metabolic Profile

An Antisense yycF RNA Modulates Biofilm Organization of Methicillin-Resistant Staphylococcus aureus and Pathogenicity in a Rat Model of Osteomyelitis

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