Two-Component Response Regulators from Arabidopsis thaliana Contain a Putative DNA-Binding Motif

Sakai, Hisashi; Aoyama, Takashi; Bono, Hidemasa; Oka, Atsuhiro

doi:10.1093/oxfordjournals.pcp.a029325

Cited by 92 publications

(77 citation statements)

References 29 publications

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“…In other words, this B motif of ‫ف‬ 60 amino acids is a signature of the type-B family of ARRs. It also is related to plant GARP motifs, as emphasized in the Introduction (Riechmann et al, 2000), and its inferred consensus sequence shows similarity to the so-called plant Myb-related domains that are found in many plant proteins (Sakai et al, 1998). These include the well-characterized CCA1 (circadian clock-associated protein) and LHY (late elongated hypocotyl), both of which were classified as single Myb-related transcription factors of Arabidopsis (Schaffer et al, 1998;Wang and Tobin, 1998) (Figure 1A).…”

Section: Structure and Function Of The Type-b Family Of Arrsmentioning

confidence: 83%

Molecular Structure of the GARP Family of Plant Myb-Related DNA Binding Motifs of the Arabidopsis Response Regulators

et al. 2002

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The B motif is a signature of type-B response regulators (ARRs) involved in His-to-Asp phosphorelay signal transduction systems in Arabidopsis. Homologous motifs occur widely in the GARP family of plant transcription factors. To gain general insight into the structure and function of B motifs (or GARP motifs), we characterized the B motif derived from a representative ARR, ARR10, which led to a number of intriguing findings. First, the B motif of ARR10 (named ARR10-B and extending from Thr-179 to Ser-242) possesses a nuclear localization signal, as indicated by the intracellular localization of a green fluorescent protein-ARR10-B fusion protein in onion epidermal cells. Second, the purified ARR10-B molecule binds specifically in vitro to DNA with the core sequence AGATT. This was demonstrated by several in vitro approaches, including PCR-assisted DNA binding site selection, gel retardation assays, and surface plasmon resonance analysis. Finally, the three-dimensional structure of ARR10-B in solution was determined by NMR spectroscopy, showing that it contains a helix-turn-helix structure. Furthermore, the mode of interaction between ARR10-B and the target DNA was assessed extensively by NMR spectroscopy. Together, these results lead us to propose that the mechanism of DNA recognition by ARR10-B is essentially the same as that of homeodomains. We conclude that the B motif is a multifunctional domain responsible for both nuclear localization and DNA binding and suggest that these insights could be applicable generally to the large GARP family of plant transcription factors.

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Section: Structure and Function Of The Type-b Family Of Arrsmentioning

confidence: 83%

Molecular Structure of the GARP Family of Plant Myb-Related DNA Binding Motifs of the Arabidopsis Response Regulators

et al. 2002

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“…The type-A RRs are cytokinin primary response genes whose proteins consist mostly of a receiver domain with conserved D-D-K residues (Brandstatter and Kieber, 1998;Taniguchi et al, 1998;D'Agostino et al, 2000). The type-B RRs are more complex proteins that contain, in addition to the receiver domain, a DNA-binding motif (GARP domain) that resembles a domain originally found in the mammalian oncoprotein c-Myb (Imamura et al, 1998;Sakai et al, 1998). While phosphorylated type-B RRs act as transcriptional activators of cytokinin-regulated genes, the type-A RRs down-regulate cytokinin signaling by competing with the type-B RRs for phosphoryl groups (for review, see To and Kieber, 2008).…”

mentioning

confidence: 99%

The Cytokinin Type-B Response Regulator PtRR13 Is a Negative Regulator of Adventitious Root Development inPopulus

et al. 2009

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Adventitious root formation at the base of plant cuttings is an innate de novo organogenesis process that allows massive vegetative propagation of many economically and ecologically important species. The early molecular events following shoot excision are not well understood. Using whole-genome microarrays, we detected significant transcriptome remodeling during 48 h following shoot removal in Populus tremula 3 Populus alba softwood cuttings in the absence of exogenous auxin, with 27% and 36% of the gene models showing differential abundance between 0 and 6 h and between 6 and 24 h, respectively. During these two time intervals, gene networks involved in protein turnover, protein phosphorylation, molecular transport, and translation were among the most significantly regulated. Transgenic lines expressing a constitutively active form of the Populus type-B cytokinin response regulator PtRR13 (DDDKPtRR13) have a delayed rooting phenotype and cause misregulation of CONTINUOUS VASCULAR RING1, a negative regulator of vascularization; PLEIOTROPIC DRUG RESISTANCE TRANS-PORTER9, an auxin efflux transporter; and two APETALA2/ETHYLENE RESPONSE FACTOR genes with sequence similarity to TINY. Inappropriate cytokinin action via DDDKPtRR13 expression appeared to disrupt adventitious root development 24 h after shoot excision, when root founder cells are hypothesized to be sensitive to the negative effects of cytokinin. Our results are consistent with PtRR13 acting downstream of cytokinin to repress adventitious root formation in intact plants, and that reduced cytokinin signaling after shoot excision enables coordinated expression of ethylene, auxin, and vascularization pathways leading to adventitious root development.

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“…The mammalian Myb generally consists of three repeats, each of which contains three distinctive modules that respectively center on a tryptophan residue (Ogata et al, 1992). In contrast, ARRM contains one repeat, and the conserved tryptophan residues in the second and third modules are replaced by either alanine or isoleucine residues in the cases of ARR1 and ARR2 (Sakai et al, 1998). ARRM binds double-stranded DNA in a sequence-specific manner in vitro , although initially this characteristic was in doubt, since ARRM has lowlevel homology to Myb.…”

Section: Structure and Biochemical Characteristics Of The Arabidopsismentioning

confidence: 99%

“…We found that a considerable number of response regulator genes were distributed among the plant genomes. Furthermore, these loci encoded proteins that could be classified into two groups: the A-type proteins were relatively small molecules that seemed to consist of only a signal receiver domain and an extremely short stretch at the C-terminus, whereas the B-type proteins contained additional functional domains after the signal receiver domain (Imamura et al, 1998;Sakai et al, 1998). Since the entire Arabidopsis genome sequence is now available (The Arabidopsis Genome Initiative, 2000), we know that Arabidopsis carries eleven genes for each of the A-type and B-type response regulators ( ARR1 to ARR22 ), eleven sensor histidine kinase genes ( AHK ), and five HPt protein genes ( AHP1 to AHP5 ).…”

Section: Introductionmentioning

confidence: 99%

His-Asp phosphorelay signal transduction in higher plants: Receptors and response regulators for cytokinin signaling in Arabidopsis thaliana.

2002

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Bacteria have devised phosphotransfer signaling mechanisms for eliciting a variety of adaptive responses to their environment. These mechanisms are collectively referred to as two-component regulatory systems. Each system generally consists of a sensor protein histidine kinase, which is anchored in the cell membrane, and a cytoplasmic response regulator, whose activity is modulated by the sensor. Most response regulators are transcription factors. In this review, we briefly introduce the established concept on bacterial two-component regulatory systems, using the Agrobacterium VirA-VirG system as an example, and give the evidence for the existence of quite similar systems in higher plants, such as the signal transduction induced by the phytohormone cytokinin. The Arabidopsis CRE1 histidine kinase and its related proteins AHK2 and AHK3 perceive cytokinins in the environment and transduce a signal, presumably through the AHP bridge components that carry the histidine-containing phosphotransfer (HPt) domain, to the ARR1 response regulator that transcriptionally activates genes immediately responsive to cytokinins. In addition, this signal transfer process appears to participate in cross-talk with signaling systems that respond to daylight and another phytohormone, ethylene, through an intracellular pool of several ARR1-like molecular species and the AHP components.

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Two-Component Response Regulators from Arabidopsis thaliana Contain a Putative DNA-Binding Motif

Cited by 92 publications

References 29 publications

Molecular Structure of the GARP Family of Plant Myb-Related DNA Binding Motifs of the Arabidopsis Response Regulators

Molecular Structure of the GARP Family of Plant Myb-Related DNA Binding Motifs of the Arabidopsis Response Regulators

The Cytokinin Type-B Response Regulator PtRR13 Is a Negative Regulator of Adventitious Root Development inPopulus

His-Asp phosphorelay signal transduction in higher plants: Receptors and response regulators for cytokinin signaling in Arabidopsis thaliana.

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