Two common SNPs in pri-miR-125a alter the mature miRNA expression and associate with recurrent pregnancy loss in a Han-Chinese population

Hu, Yi; Liu, Chunmei; Qi, Lu; He, Tian-Zhu; Liu, Shiguo; Hao, Chanjuan; Cui, Yi; Zhang, Ning; Xia, Hong-Fei; Ma, Xu

doi:10.4161/rna.8.5.16034

Cited by 94 publications

(97 citation statements)

References 27 publications

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“…Many factors such as, uterine irregularity, chromosomal anomalies, endocrine dysfunctions, hematopoietic disease, immunological dysfunctions, lifestyle, and chronic maternal diseases, have been described that correlated to the abortion (2). The previous investigation indicated that two to seven-fold increase prevalence of RPL between the firstdegree blood relatives compared to the background community (3).…”

Section: Introductionmentioning

confidence: 99%

“…The previous investigation indicated that two to seven-fold increase prevalence of RPL between the firstdegree blood relatives compared to the background community (3). Unexplained RPL is a stressful status of the couple, which assistance effort to overwhelming of the matter (2). It is estimated that approximately 50% of etiology of RPL cases remain unexplained.…”

Section: Introductionmentioning

confidence: 99%

“…miRNAs are a different group of short non-coding RNAs, which play crucial functions in all of the organism genomes. miRNAs are species of 20 -24 nucleotide short RNAs, which negatively regulated gene expression on the post-transcriptional level (2,3). miRNAs play as a leader molecule in post-transcriptional gene silencing through base pairing with the purpose of mRNAs, which leads to the mRNA cleavage or translation suppression.…”

Section: Introductionmentioning

confidence: 99%

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Association Study of Non-Coding RNA miR-499 and miR196a2 Gene Polymorphisms with the Risk of Idiopathic Recurrent Pregnancy Loss

Fazli

Ghorbian

2018

Gene Cell Tissue

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Background: MicroRNAs (miRNAs) play significant roles in several physiological procedures such as embryo implantation. Recently, findings have indicated that aberrant miRNAs gene expression and single nucleotide polymorphism in the gene encoded miRNAs may be contributed to the pathogenesis of recurrent pregnancy loss (RPL). This investigation aimed to examine potential associations between the two precursor miRNA SNPs miR-196a2 T > C and miR-499 A > G and susceptibility to RPL. Methods: We were analyzing a total of 200 subjects, which consisted of 100 women who experienced three or more recurrent miscarriages and 100 healthy women with one child and without historical aberrations. The genotypic frequencies of two SNPs were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to assess the association between the presence miRNA gene variant with the risk of RPL. Results: The genotypes frequency of miR-499 gene polymorphism AA/AG + GG and GG/AG + AA showed a statistically significant association with the risk of RPL (P = 0.019; OR = 2.003; 95% CI, 1.116 -3.594) and (P = 0.024; OR = 2.100; 95% CI, 1.095 -4.025), respectively. The genotypes frequency of miR-196a2 gene polymorphisms did not reveal a statistically significant difference across the two groups (P > 0.05). Conclusions: Our findings proposed that nucleotide changes in miR-499 gene encoding region may be contributed as a genetic predisposition factor to RPL, whereas the miR-196a2 gene variation cannot be a negative effect on pregnancy outcome.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Association Study of Non-Coding RNA miR-499 and miR196a2 Gene Polymorphisms with the Risk of Idiopathic Recurrent Pregnancy Loss

Fazli

Ghorbian

2018

Gene Cell Tissue

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“…Although the mature MIR423 level was lower in the C/A heterozygosity of rs6505162 than in the A allele, it was significantly increased by the C/A heterozygosity of rs6505162 as compared to the CC homozygote. These results suggest that the rare allele A in pre-MIR423 contributes to the production of mature MIR423, which is similar to the polymorphisms of MIR125A and has been shown to be associated with an increased risk of RPL in the Han Chinese population (Hu et al 2011). The rs6505162 C OA in MIR423 has been identified as a biomarker for many diseases.…”

Section: Discussionmentioning

confidence: 55%

“…Increasing evidence has shown that single nucleotide polymorphisms (SNPs) or mutations in miRNA coding regions may alter miRNA expression and/ or maturation, which may affect the occurrence of diseases (Saunders et al 2007, Jazdzewski et al 2008. Our previous report (Hu et al 2011) showed that two SNP sites in the pre-MIR125A coding region are related to human RPL, which means that miRNAs might play an important role in the occurrence of RPL.…”

Section: Introductionmentioning

confidence: 99%

The polymorphism of rs6505162 in the MIR423 coding region and recurrent pregnancy loss

Su¹,

Hu²,

Liu³

et al. 2015

Reproduction

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Although the relationship between polymorphisms in microRNAs (miRNAs) and recurrent pregnancy loss (RPL) has been studied, there is very little data available in the literature. In the present study, we scanned 55 potentially functional polymorphisms in the miRNA coding region in Chinese women with unexplained RPL (URPL; no. 2011-10). The rs6505162 COA in the MIR423 coding region was found to be significantly associated with the occurrence of human URPL. The rare A allele contributed to an increase in the expression of mature MIR423. C to A substitution in the polymorphism rs6505162 in pre-MIR423 repressed cell proliferation and migratory capacity. Further investigations showed that MIR423 could inversely regulate the expression of proliferation-associated 2 group 4 (PA2G4) by binding the 3 0 -UTR of PA2G4. Dual-luciferase assay indicated that the A allele in the polymorphism rs6505162 could more effectively suppress the expression of PA2G4 than the C allele could. Collectively, the present data suggest that rs6505162 COA in pre-MIR423 may contribute to the genetic predisposition to RPL by disrupting the production of mature MIR423 and its target gene, which consequently interferes with MIR423 functioning.Reproduction (2015) 150 65-76

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MiR‐101 inhibits ovarian carcinogenesis by repressing the expression of brain‐derived neurotrophic factor

Zheng

et al. 2017

FEBS Open Bio

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Ovarian cancer is one of the most lethal malignant gynecological tumors as a result of difficulties in early‐stage detection and a lack of effective treatments for patients with advanced or recurrent cancer. In the present study, we aimed to explore whether some of the micro RNA (mi RNA ) content of serum might be related to ovarian cancer, as well as the role of these mi RNA s and their intercellular transport via exosomes in ovarian cancer. We first detected the expression of six candidate mi RNA s in ovarian cancer tissues and adjacent nontumor ovarian samples from 36 patients and confirmed the altered expression of four mi RNA s. The level of these six candidate mi RNA s was also examined in exosomes from patient serum samples. Only the level of miR‐101 was altered in both ovarian tissue samples and serum exosomes. After prediction using online bioinformatics tools and confirmation by dual‐luciferase assay and immunoblotting, we identified that miR‐101 can repress the expression of brain‐derived neurotrophic factor by targeting its 3′‐ UTR . Using Transwell assays, we examined the effect of miR‐101 on migration and invasion capacity of ovarian cancer cells. The results indicated that the reduction of miR‐101 is mostly related to significant enhanced ovarian cancer cell migration. Thus, the results of the present study indicate that miR‐101 content in serum exosomes has potential as a marker for diagnosis of ovarian cancer and that miR‐101 mimics are potential therapeutic drugs for the treatment of ovarian cancer.

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Two common SNPs in pri-miR-125a alter the mature miRNA expression and associate with recurrent pregnancy loss in a Han-Chinese population

Abstract: (2011) Two common SNPs in pri-miR-125a alter the mature miRNA expression and associate with recurrent pregnancy loss in a Han-Chinese population, RNA Biology, 8:5,[861][862][863][864][865][866][867][868][869][870][871][872]

Cited by 94 publications

References 27 publications

Association Study of Non-Coding RNA miR-499 and miR196a2 Gene Polymorphisms with the Risk of Idiopathic Recurrent Pregnancy Loss

Association Study of Non-Coding RNA miR-499 and miR196a2 Gene Polymorphisms with the Risk of Idiopathic Recurrent Pregnancy Loss

The polymorphism of rs6505162 in the MIR423 coding region and recurrent pregnancy loss

MiR‐101 inhibits ovarian carcinogenesis by repressing the expression of brain‐derived neurotrophic factor

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