Two chloroplast‐localized <scp>MORF</scp> proteins act as chaperones to maintain tetrapyrrole biosynthesis

Yuan, Jiarui; Ma, Tingting; Ji, Shuiling; Hedtke, Boris; Grimm, Bernhard; Lin, Rongcheng

doi:10.1111/nph.18273

Cited by 10 publications

(11 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, we predicted that more MORF2binding partners could be discovered in the future. Further supporting our discovery about the role of MORF2 in RS beyond RNA-editing, multiple MORF2-interacting enzymes and regulators involved in tetrapyrrole biosynthesis do not affect plastid RNA editing either (Yuan et al, 2022).…”

Section: Chloroplasts Generate Multiple Rs Pathways Directing Complex...supporting

confidence: 70%

“…In this work, we applied a novel inducible Clustered Regularly Interspaced Short Palindromic Repeat interference (iCRISPRi) approach to quantitatively monitor early transcriptome and RNA-editing changes upon the gradient reduction of MORF2. Further physiological and expression analyses, in combination with the structure feature and a recent finding of a holdase-like chaperon activity of MORF2 ( Yuan et al., 2022 ), collectively suggest that MORF2 is a stress effector in addition to its previously discovered role in RNA editing.…”

Section: Introductionmentioning

confidence: 81%

“…The essential role of MORF2 in RS could be attributed to its large array of binding partners in chloroplasts, such as MORF proteins ( Zehrmann et al., 2015 ; Huang et al., 2019 ), PPR proteins involved in RNA-editing ( Takenaka et al., 2012 ), GUN1 ( Zhao et al., 2019 ), and multiple enzymes and regulators involved in tetrapyrrole biosynthesis ( Zhang et al., 2014 ; Yuan et al., 2022 ). Its large binding capacity is consistent with its structural feature that can be divided into an N-terminal intrinsically disordered region and a peptidase inhibitor I9 domain-containing C-terminal region ( Luo et al., 2017 ; Varadi et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the expression induction of dCas9-KARB and suppression of MORF2 were detected in accordance with seedling growth inhibition (Figures 2D, E). This result was inconsistent with the well-known RNA-editing function of MORF2 (Yuan et al, 2022). To carefully examine this discrepancy, we developed a transcriptome-wide analysis of chloroplast RNA-editing efficiency.…”

Section: Morf2 Reduction Suppresses Plant Growth In Addition To Affec...mentioning

confidence: 90%

See 3 more Smart Citations

MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing

et al. 2023

View full text Add to dashboard Cite

Retrograde signaling modulates the expression of nuclear genome-encoded organelle proteins to adjust organelle function in response to environmental cues. MULTIPLE ORGANELLAR RNA EDITING FACTOR 2 (MORF2) was initially recognized as a plastidial RNA-editing factor but recently shown to interact with GUN1. Given the central role of GUN1 in chloroplast retrograde signaling and the unviable phenotype of morf2 mutants that is inconsistent with many viable mutants involved in RNA editing, we hypothesized that MORF2 has functions either dosage dependent or beyond RNA editing. Using an inducible Clustered Interspaced Short Palindromic Repeat interference (iCRISPRi) approach, we were able to reduce the MORF2 transcripts in a controlled manner. In addition to MORF2-dosage dependent RNA-editing errors, we discovered that reducing MORF2 by iCRISPRi stimulated the expression of stress responsive genes, triggered plastidial retrograde signaling, repressed ethylene signaling and skotomorphogenesis, and increased accumulation of hydrogen peroxide. These findings along with previous discoveries suggest that MORF2 is an effective regulator involved in plastidial metabolic pathways whose reduction can readily activate multiple retrograde signaling molecules possibly involving reactive oxygen species to adjust plant growth. In addition, our newly developed iCRISPRi approach provided a novel genetic tool for quantitative reverse genetics studies on hub genes in plants.

show abstract

Section: Chloroplasts Generate Multiple Rs Pathways Directing Complex...supporting

confidence: 70%

Section: Introductionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Morf2 Reduction Suppresses Plant Growth In Addition To Affec...mentioning

confidence: 90%

See 2 more Smart Citations

MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The entire Chl biosynthesis process starts from L-glutamine-tRNA and ultimately forms Chl a and Chl b through a multistep enzymatic reaction involving 15 enzymes and more than 20 genes [ 9 , 10 ]. During this process, some transcriptional regulators also strongly affected the efficiency of chlorophyll synthesis and the development of chloroplasts, such as the LONG HYPOCOTYL 5 (HY5) protein [ 11 ], multiple organellar RNA editing factors (MORFs) [ 12 ], and the NAC transcription factor (TF) [ 13 ], WRKY [ 14 ], and MYB families [ 15 ]. Additionally, studies have reported that the expression of some genes related to chloroplasts also affect their biogenesis.…”

Section: Introductionmentioning

confidence: 99%

Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco

Feng

Zhao

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

Background Photosynthetic pigments in higher plants, including chlorophyll (Chl) and carotenoids, are crucial for photosynthesis and photoprotection. Chl-deficient tobacco seedlings generally have a lower photosynthesis rate and higher nitrate-nitrogen (NO3-N) content, which causes a profound influence on tobacco yield and quality. In this study, a stable albino leaf mutant (Al) and slight-green leaf mutant (SG) obtained from the common flue-cured tobacco (Nicotiana tabacum L.) cultivar ‘Zhongyan 100’ (ZY100) by mutagenesis with ethyl methanesulfonate (EMS) were used as materials. The differences between the Chl-deficient mutants and the wild-type (WT) were analyzed in terms of biomass, photosynthetic fluorescence parameters, and carbon- and nitrogen-related physiological parameters. RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA) were used to explore the key pathways and candidate genes regulating differentiated chlorophyll and nitrate content. Results The results showed that, when compared to the WT, the Chl content and biomass of mutant plants were considerably lower while the NO3-N content was substantially elevated. The net photosynthetic rate, photosynthetic fluorescence parameters, carbohydrate, soluble protein, and carbon- and nitrogen-related enzyme activities all decreased in leaves of mutants and the development of chloroplasts was abnormal. Applying more nitrogen improved the growth and development of mutants, whereas NO3-N content distinctively increased compared with that of the WT. Through transcriptome sequencing, the downregulated genes in mutants were enriched in plant hormone signal transduction and nitrogen metabolism, which are involved in pigment biosynthesis and the carbon fixation pathway. In addition, two hub genes and seven transcription factors identified from the blue module through WGCNA were likely to be key candidate factors involved in chlorophyll synthesis and nitrate accumulation. Conclusion Our results demonstrated that differences in chlorophyll and nitrate content were caused by the combined effects of chloroplast development, photosynthesis, as well as related biological activity. In addition, transcriptome results provide a bioinformatics resource for further functional identification of key pathways and genes responsible for differences in chlorophyll and nitrate content in tobacco plants.

show abstract

Lysine 2‐Hydroxyisobutyrylation‐ and Succinylation‐Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity

et al. 2023

View full text Add to dashboard Cite

Crops must efficiently allocate their limited energy resources to survival, growth and reproduction, including balancing growth and defense. Thus, investigating the underlying molecular mechanism of crop under stress is crucial for breeding. Chloroplasts immunity is an important facet involving in plant resistance and growth, however, whether and how crop immunity modulated by chloroplast is influenced by epigenetic regulation remains unclear. Here, the cotton lysine 2‐hydroxyisobutyrylation (Khib) and succinylation (Ksuc) modifications are firstly identified and characterized, and discover that the chloroplast proteins are hit most. Both modifications are strongly associated with plant resistance to Verticillium dahliae, reflected by Khib specifically modulating PR and salicylic acid (SA) signal pathway and the identified GhHDA15 and GhSRT1 negatively regulating Verticillium wilt (VW) resistance via removing Khib and Ksuc. Further investigation uncovers that photosystem repair protein GhPSB27 situates in the core hub of both Khib‐ and Ksuc‐modified proteins network. The acylated GhPSB27 regulated by GhHDA15 and GhSRT1 can raise the D1 protein content, further enhancing plant biomass‐ and seed‐yield and disease resistance via increasing photosynthesis and by‐products of chloroplast‐derived reactive oxygen species (cROS). Therefore, this study reveals a mechanism balancing high disease resistance and high yield through epigenetic regulation of chloroplast protein, providing a novel strategy to crop improvements.

show abstract

Two chloroplast‐localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis

Cited by 10 publications

References 61 publications

MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing

MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing

Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco

Lysine 2‐Hydroxyisobutyrylation‐ and Succinylation‐Based Pathways Act Inside Chloroplasts to Modulate Plant Photosynthesis and Immunity

Contact Info

Product

Resources

About