Two Cells, One Antibody: The Discovery of the Cellular Origins and Transport of Secretory IgA

Kaetzel, Charlotte S.; Městecký, Jiří; Johansen, Finn–Eirik

doi:10.4049/jimmunol.1700025

Cited by 11 publications

(9 citation statements)

References 51 publications

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“…One other innate antimicrobial activity ascribed to mammalian IEC is to use free secretory component (FSC) to bind luminal bacteria. FSC is formed by cleavage of unbound pIgR at the apical side of enterocytes [ 31 , 32 ]. To investigate a similar role for the secretory component in the chicken we used secretory component specific immunohistochemistry to study its expression in intestinal tissue; the chicken homologue (GG-pIgR) is bound by anti-human secretory component [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Innate immune functions of avian intestinal epithelial cells: Response to bacterial stimuli and localization of responding cells in the developing avian digestive tract

Shira

Friedman

2018

PLoS ONE

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Intestinal epithelial cells are multi-tasked cells that participate in digestion and absorption as well as in protection of the digestive tract. While information on the physiology and immune functions of intestinal epithelial cells in mammals is abundant, little is known of their immune function in birds and other species. Our main objectives were to study the development of anti-bacterial innate immune functions in the rapidly developing gut of the pre- and post-hatch chick and to determine the functional diversity of epithelial cells. After establishing primary intestinal epithelial cell cultures, we demonstrated their capacity to uptake and process bacteria. The response to bacterial products, LPS and LTA, induced expression of pro-inflammatory cytokine genes (IL-6, IL-18) as well as the expression of the acute phase proteins avidin, lysozyme and the secretory component derived from the polymeric immunoglobulin receptor. These proteins were then localized in gut sections, and the goblet cell was shown to store avidin, lysozyme as well as secretory component. Lysozyme staining was also located in a novel rod-shaped intestinal cell, situated at different loci along the villus, thus deviating from the classical Paneth cell in the mammal, that is restricted to crypts. Thus, in the chicken, the intestinal epithelium, and particularly goblet cells, are committed to innate immune protection. The unique role of the goblet cell in chicken intestinal immunity, as well as the unique distribution of lysozyme-positive cells highlight alternative solutions of gut protection in the bird.

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Section: Resultsmentioning

confidence: 99%

Innate immune functions of avian intestinal epithelial cells: Response to bacterial stimuli and localization of responding cells in the developing avian digestive tract

Shira

Friedman

2018

PLoS ONE

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“…J chain is a small polypeptide to form pentameric IgM and dimeric IgA, but little is known about the function of J chain due to the technical limitation [15]. When one dIgA is bound to the polymeric immunoglobulin receptor (pIgR) at the basolateral side of the epithelium thereby transported to the luminal side, the dIgA-binding portion of the pIgR is cleaved to form the molecule sIgA [16]. e pIgR fragment of sIgA is called secretory component (SC) to support the stability of sIgA [17].…”

Section: Structure Of Sigamentioning

confidence: 99%

The Effects of Secretory IgA in the Mucosal Immune System

Jin

Chen

2020

BioMed Research International

178

148

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Immunoglobulin A (IgA) is the most abundant antibody isotype in the mucosal immune system. Structurally, IgA in the mucosal surface is a polymeric structure, while serum IgA is monomeric. Secretory IgA (sIgA) is one of the polymeric IgAs composed of dimeric IgA, J chain, and secretory component (SC). Most of sIgAs were generated by gut and have effects in situ. Besides the function of “immune exclusion,” a nonspecific immune role, recent studies found it also played an important role in the specific immunity and immunoregulation. Thanks to the critical role of sIgA during the mucosal immune system homeostasis between commensal microorganisms and pathogens; it has been an important field exploring the relationship between sIgA and commensal microorganisms.

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“…After accomplishing the uptake, pIgR-dIgA and pIgR-pIgA complexes are transported via vesicle-mediated transcytosis. After reaching the apical epithelial surface, pIgR is broken down to generate a secretory component, so that the protein complexes are released as secretory IgA (SIgA) at the lumen [ 7 ]. dIgA and pIgR show an expression gradient, lower at the duodenum and higher at the colon [ 10 ].…”

Section: An Overview Of the Biochemical And Immune Gut Barrier Componentsmentioning

confidence: 99%

“…The epithelial monolayer determines gut permeability, namely the rate of flux of molecules across the epithelium [ 6 ]. The epithelial monolayer expresses the polymeric immunoglobulin receptor (pIgR) at the basolateral surface; this protein allows for immunoglobulin A (IgA) transport [ 7 ]. The final component is an immune barrier comprising gut-associated lymphoid tissue, including immunocompetent cells (T, IgA+ B, dendritic, and IgA+ plasma cells, among others) and humoral effectors such as IgA [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Intestinal Homeostasis under Stress Siege

Guzmán-Mejía

Godínez‐Victoria

Vega-Bautista

et al. 2021

IJMS

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Intestinal homeostasis encompasses a complex and balanced interplay among a wide array of components that collaborate to maintain gut barrier integrity. The appropriate function of the gut barrier requires the mucus layer, a sticky cushion of mucopolysaccharides that overlays the epithelial cell surface. Mucus plays a critical anti-inflammatory role by preventing direct contact between luminal microbiota and the surface of the epithelial cell monolayer. Moreover, mucus is enriched with pivotal effectors of intestinal immunity, such as immunoglobulin A (IgA). A fragile and delicate equilibrium that supports proper barrier function can be disturbed by stress. The impact of stress upon intestinal homeostasis results from neuroendocrine mediators of the brain-gut axis (BGA), which comprises a nervous branch that includes the enteric nervous system (ENS) and the sympathetic and parasympathetic nervous systems, as well as an endocrine branch of the hypothalamic-pituitary-adrenal axis. This review is the first to discuss the experimental animal models that address the impact of stress on components of intestinal homeostasis, with special emphasis on intestinal mucus and IgA. Basic knowledge from animal models provides the foundations of pharmacologic and immunological interventions to control disturbances associated with conditions that are exacerbated by emotional stress, such as irritable bowel syndrome.

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Two Cells, One Antibody: The Discovery of the Cellular Origins and Transport of Secretory IgA

Cited by 11 publications

References 51 publications

Innate immune functions of avian intestinal epithelial cells: Response to bacterial stimuli and localization of responding cells in the developing avian digestive tract

Innate immune functions of avian intestinal epithelial cells: Response to bacterial stimuli and localization of responding cells in the developing avian digestive tract

The Effects of Secretory IgA in the Mucosal Immune System

Intestinal Homeostasis under Stress Siege

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