Two cell wall associated peroxidases from Arabidopsis influence root elongation

Passardi, Filippo; Tognolli, Michael; Meyer, Mireille De; Penel, Claude; Dunand, Christophe

doi:10.1007/s00425-005-0153-4

Cited by 164 publications

(139 citation statements)

References 30 publications

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“…S9). Peroxidases, which can function to polymerize cell wall compounds, were also up-regulated in TZP-OX, consistent with their role in growth and cell wall expansion (26). Peroxidases PER27, PER30, and PER64 have been shown to be part of the cell wall proteome (27) (six PER genes total; Fig.…”

Section: Tzp Controls Morning-specific Growth Through An Auxin-relatedsupporting

confidence: 55%

A zinc knuckle protein that negatively controls morning-specific growth in Arabidopsis thaliana

Loudet

Michael

Burger

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Growth in plants is modulated by a complex interplay between internal signals and external cues. Although traditional mutagenesis has been a successful approach for the identification of growth regulatory genes, it is likely that many genes involved in growth control remain to be discovered. In this study, we used the phenotypic variation between Bay-0 and Shahdara, two natural strains (accessions) of Arabidopsis thaliana, to map quantitative trait loci (QTL) affecting light-and temperature-regulated growth of the embryonic stem (hypocotyl). Using heterogeneous inbred families (HIFs), the gene underlying one QTL, LIGHT5, was identified as a tandem zinc knuckle/PLU3 domain encoding gene (At5g43630; TZP), which carries a premature stop codon in Bay-0. Hypocotyl growth assays in monochromatic light and microarray analysis demonstrate that TZP controls blue light associated growth in a time-of-day fashion by regulating genes involved in growth, such as peroxidase and cell wall synthesis genes. TZP expression is phased by the circadian clock and light/dark cycles to the beginning of the day, the time of maximal growth in A. thaliana in short-day conditions. Based on its domain structure and localization in the nucleus, we propose that TZP acts downstream of the circadian clock and photoreceptor signaling pathways to directly control genes responsible for growth. The identification of TZP thus provides new insight into how daily synchronization of growth pathways plays a critical role in growth regulation.blue light ͉ circadian ͉ fine-mapping ͉ quantitative T he embryonic stem or hypocotyl is an excellent model for studying both internal and external factors controlling growth in plants (1). Genetic screens in common laboratory accessions have yielded direct molecular insight into how light-and hormonedependent signaling pathways interact with the circadian clock to regulate the final length of the hypocotyl (1). The power of the hypocotyl assay is its simplicity, as well as its obvious meaningfulness. When germinating seeds are exposed to low levels of light, such as those caused by a covering layer of debris, the hypocotyl has to grow for a while. Only after the surface has been broken by the tip of the hypocotyls can the embryonic leaves, the cotyledons, unfold. Conversely, if a seed has fallen on open ground, there is no need for the hypocotyls to be particularly long. Because of the ease and reproducibility with which hypocotyl length can be measured in thousands of individuals, it has also been a powerful model in mapping genes with more subtle effects on light and hormone regulated growth, by using methods of quantitative genetics (2). Multiple light signaling genes controlling hypocotyl length have been characterized in quantitative trait locus (QTL) studies (3-6).In this study, we use the hypocotyl assay to identify QTL controlling growth in 2 light and 2 temperature conditions. We identified a recessive large effect QTL on chromosome five controlling 40% of the growth variation segregating in Recombinant Inb...

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Section: Tzp Controls Morning-specific Growth Through An Auxin-relatedsupporting

confidence: 55%

A zinc knuckle protein that negatively controls morning-specific growth in Arabidopsis thaliana

Loudet

Michael

Burger

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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“…Studies of individual AtPrx genes suggest that they may be involved in both cell growth and lignification. For instance, abnormal root lengths of the atprx33 and atprx34 mutants point to a role for the corresponding wild-type genes in cell elongation (Passardi et al, 2006), and expression patterns for AtPrx47, AtPrx64, and AtPrx66 are consistent with roles in vessel (AtPrx47, AtPrx64) and sclerenchyma (AtPrx64) lignification (Tokunaga et al, 2009).…”

Section: Peroxidases and Laccasesmentioning

confidence: 93%

The Phenylpropanoid Pathway in Arabidopsis

Fraser

Chapple

2011

The Arabidopsis Book

559

480

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The phenylpropanoid pathway serves as a rich source of metabolites in plants, being required for the biosynthesis of lignin, and serving as a starting point for the production of many other important compounds, such as the flavonoids, coumarins, and lignans. In spite of the fact that the phenylpropanoids and their derivatives are sometimes classified as secondary metabolites, their relevance to plant survival has been made clear via the study of Arabidopsis and other plant species. As a model system, Arabidopsis has helped to elucidate many details of the phenylpropanoid pathway, its enzymes and intermediates, and the interconnectedness of the pathway with plant metabolism as a whole. These advances in our understanding have been made possible in large part by the relative ease with which mutations can be generated, identified, and studied in Arabidopsis. Herein, we provide an overview of the research progress that has been made in recent years, emphasizing both the genes (and gene families) associated with the phenylpropanoid pathway in Arabidopsis, and the end products that have contributed to the identification of many mutants deficient in the phenylpropanoid metabolism: the sinapate esters.

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“…Recently it was shown that two homologous cell wall-located enzymes POX33 and POX34 are involved in growth-promoting local redox changes in Arabidopsis roots (Passardi et al 2006). Moreover, it was shown that NADPH oxidases are engaged in ROS generation participating in process of the microtubule depolymerization and organization of the cytoskeleton during root hairs growth (Yao et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

Libik-Konieczny

Kozieradzka-Kiszkurno

Michalec-Warzecha

et al. 2017

Acta Physiol Plant

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The enrichment with antioxidants (glutathione or ascorbate) or prooxidants (alloxan, methylviologen, hydrogen peroxide) of root inducing medium significantly decreased rhizogenesis frequency (alloxan, hydrogen peroxide) or inhibited roots regeneration (ascorbate, methylviologen) during the in vitro culture of Mesembryanthemum crystallinum L. hypocotyls. The adventitious roots morphology, root hairs length and density, was also influenced. Changes in the rhizogenesis course were related to the differences in hydrogen peroxide concentration during following days of culture between explants exhibiting morphogenic potential and those without the ability to form adventitious roots. In explants with morphogenic potential, rhizogenesis induction was always accompanied by a high level of hydrogen peroxide followed by the decrease in H 2 O 2 content in following days. In contrast, in the explants without regeneration potential, the level of hydrogen peroxide was increasing during the culture period. Activity patterns of superoxide dismutase (SOD) and guaiacol peroxidase (POX) in the following days of culture were similar in the explants exhibiting regeneration potential cultured on different media. Total activity of SOD decreased during initial days of culture and then increased due to the activation of additional SOD isoform described as MnSODII. The activity of POX was low during the rhizogenesis induction, and then increased during following days of culture; the increase was correlated with the decrease in hydrogen peroxide content. In the explants without the ability to regenerate roots, the total activity of SOD was low throughout the whole culture period, whereas the POX activity was significantly higher than in hypocotyls with regeneration potential. It might be concluded that the increase in hydrogen peroxide during initial stages of rhizogenesis and the induction of MnSODII are prerequisites for adventitious roots formation from hypocotyls of M. crystallinum, independently in the presence of anti-or prooxidant in the culture medium.

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Two cell wall associated peroxidases from Arabidopsis influence root elongation

Cited by 164 publications

References 30 publications

A zinc knuckle protein that negatively controls morning-specific growth in Arabidopsis thaliana

A zinc knuckle protein that negatively controls morning-specific growth in Arabidopsis thaliana

The Phenylpropanoid Pathway in Arabidopsis

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

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