Two cDNA clones (FsDhn1 and FsClo1) up-regulated by ABA are involved in drought responses in Fagus sylvatica L. seeds

Jiménez, Jesús Ángel; Alonso-Ramírez, Ana; Nicolás, Carlos

doi:10.1016/j.jplph.2007.11.013

Cited by 21 publications

(11 citation statements)

References 47 publications

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“…CBP-like genes were shown to be involved in potato tuberization (Poovaiah et al, 1996;Reddy et al, 2002). Similarly calcium-binding proteins such as CDPKs and calcineurin-B-like genes were shown to be induced by drought and salt stresses (Jimenez et al, 2008). Therefore, it remains to be seen if strict regulation of CBP-like genes are required to trigger SR formation in sweetpotato by modulating other downstream stress-related genes under drought stress (Albrecht et al, 2003;Cheong et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Effect of Drought on Storage Root Development and Gene Expression Profile of Sweetpotato under Greenhouse and Field Conditions

Solis¹,

Villordon²,

Baisakh

et al. 2014

J. Amer. Soc. Hort. Sci.

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Greenhouse and field culture systems were used to study the effect of drought conditions on the storage root (SR) formation in ‘Beauregard’ sweetpotato (Ipomoea batatas). In the greenhouse culture system, drought was simulated by withholding water for 5 and 10 days after transplanting (DAT) cuttings in dry sand. Control plants received water at planting and every 3 days thereafter. In the field studies, natural drought conditions and selective irrigation were used to impose water deprivation during the critical SR formation period. Greenhouse drought for 5 and 10 DAT reduced the number of SRs by 42% and 66%, respectively, compared with the controls. Field drought resulted in a 49% reduction in U.S. #1 SR yield compared with the irrigated condition. Quantitative real-time polymerase chain reaction (PCR) analysis showed differential expression of a set of sweetpotato transcription factors and protein kinases among greenhouse-grown plants subjected to well-watered conditions and water deficit during 5 DAT. A significant enhancement of expression was observed for known drought stress-associated genes such as an abscisic acid-responsive elements-binding factor, dehydration-responsive element-binding factor, and homeo-domain-zip proteins. Members of calcium-binding proteins showed differential expression under drought stress. For the first time it is reported that knotted1-like homeobox and BEL1-like genes showed altered expression in response to drought stress under a greenhouse condition. In summary, the results suggest that water deprivation during the SR formation period influences root development and expression patterns of stress-responsive genes and those previously found associated with SR formation in sweetpotato.

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Section: Discussionmentioning

confidence: 99%

Effect of Drought on Storage Root Development and Gene Expression Profile of Sweetpotato under Greenhouse and Field Conditions

Solis¹,

Villordon²,

Baisakh

et al. 2014

J. Amer. Soc. Hort. Sci.

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“…However, the protein is not detectable using the Western-blot method during seed maturation. Jiménez et al (2008) characterized a cDNA clone named FsDhn1 corresponding to a protein with predicted molecular mass of 20 kDa containing Y, S, and K segments, typical to dehydrins. During beech seeds' development FsDhn1 transcripts were detected at the fifth week after anthesis that then disappeared until the 18th week, but were present in dormant seeds and in larger amounts after drying (Jiménez et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Jiménez et al (2008) characterized a cDNA clone named FsDhn1 corresponding to a protein with predicted molecular mass of 20 kDa containing Y, S, and K segments, typical to dehydrins. During beech seeds' development FsDhn1 transcripts were detected at the fifth week after anthesis that then disappeared until the 18th week, but were present in dormant seeds and in larger amounts after drying (Jiménez et al 2008). Possibly the 20-kDa protein of FsDhn1 clone and 26 kDa dehydrin-like protein detected in stored beech seeds (Kalemba and Pukacka 2008) are in the same family proteins.…”

Section: Discussionmentioning

confidence: 99%

Desiccation tolerance acquisition in developing beech (Fagus sylvatica L.) seeds: the contribution of dehydrin-like protein

Kalemba

Janowiak

Pukacka

2008

Trees

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The acquisition of desiccation tolerance (DT) in developing beech (Fagus sylvatica L.) seeds and the role of a dehydrin protein in this process were investigated. DT was determined by measurement of electrolyte leakage and germination capacity after drying to 10-12% moisture content (MC). In addition to mass maturity, the presence of heat-stable proteins, dehydrin accumulation and the peak of ABA content were measured in relation to the acquisition of DT. Mass maturity was achieved at 16 weeks after flowering (WAF). The germination capacity increased from 8% at 12 WAF to 80-90% after 16 WAF. Cell membrane integrity, measured as a decrease in electrolyte leakage after desiccation, was acquired at 16 WAF. Additionally, the ratio of heat-stable to soluble proteins was the highest at 16 WAF. One dehydrin-like protein with a molecular mass 44 kDa, named DHN44, was detected in embryonic axes at 16 WAF and in cotyledons at 17 WAF, and its gradual accumulation was observed in mature seeds. With regard to the acquisition of DT, the strongest correlations were detected between electrolyte leakage, DHN44 accumulation, and the percentage of heat-stable proteins. These results suggest that developing beech seeds become tolerant to desiccation at 16 WAF. The effect of desiccation and ABA treatment on DHN44 synthesis was tested before (14 WAF) and after the DT acquisition (18 WAF).Depending on the maturation stage desiccation and ABA treatment can induce or enlarge DHN44 expression.

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“…The analysis of the variation within 'candidate' genes potentially involved in adaptation to a phenotypic trait is one possibility to investigate the genetic background of adaptation. Until now, only few studies aim to identify genes that are involved in drought stress response and bud phenology in beech (Lalagüe et al 2010), and only a limited number of beech sequences are available (Jimenez et al 2008;Olbrich et al 2005Olbrich et al , 2010Schlink 2011). Therefore, the candidate gene approach described here is based on both published F. sylvatica sequences and orthologous sequences identified in other plant species such as oaks (Gailing et al 2009;Vornam et al 2011).…”

Section: Introductionmentioning

confidence: 99%

DNA sequence variation and development of SNP markers in beech (Fagus sylvatica L.)

Seifert¹,

Vornam²,

Finkeldey³

2012

Eur J Forest Res

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European beech (Fagus sylvatica L.) is one of the most important deciduous tree species in Central Europe. The potential of beech to adapt to climate change, higher temperatures, and less precipitation in the summer months is still unknown. Most studies in beech used microsatellite, AFLP (amplified fragment length polymorphism), or isozyme markers, which have only a restricted potential to analyze adaptation. Only few studies investigated genes probably involved in the adaptation to drought stress and bud phenology in beech. In this study, SNP (single nucleotide polymorphisms) markers were developed in order to analyze adaptation and their technical advantages compared to microsatellites and AFLPs were discussed. Partial sequences of ten candidate genes probably involved in drought stress and/or bud phenology were identified at the genomic level, and SNPs and indels (insertions/deletions) in coding and non-coding regions were analyzed. Plant material was sampled along a precipitation gradient in Germany. In total, 8,145 bp were sequenced and analyzed, 4,038 bp were located in exon and 4,107 bp in intron regions. 63 SNPs and 11 indels were detected, which are differently distributed over the studied gene regions. The nucleotide diversity ranged from 0 to 6.62 (p 9 10 -3 ) and is comparable to other tree species, whereas the mean nucleotide diversity (2.64) for F. sylvatica is comparatively low. These results will help to investigate the genetic basis of drought stress and bud burst and to conduct association mapping in natural populations. Furthermore, the detected SNPs can also be used for population genetic studies.

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Two cDNA clones (FsDhn1 and FsClo1) up-regulated by ABA are involved in drought responses in Fagus sylvatica L. seeds

Cited by 21 publications

References 47 publications

Effect of Drought on Storage Root Development and Gene Expression Profile of Sweetpotato under Greenhouse and Field Conditions

Effect of Drought on Storage Root Development and Gene Expression Profile of Sweetpotato under Greenhouse and Field Conditions

Desiccation tolerance acquisition in developing beech (Fagus sylvatica L.) seeds: the contribution of dehydrin-like protein

DNA sequence variation and development of SNP markers in beech (Fagus sylvatica L.)

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