Two birds with one stone: Gold “bridging” both nanozyme and immunosensor for sensitive double-response detection of T-2 toxin

Shu, Rui; Liu, Sijie; Zhao, Cong; Lan, Xi; Li, Yuechun; Wang, Jianlong; Qiu, Nannan; Zhang, Daohong

doi:10.1016/j.cej.2023.148473

Cited by 7 publications

(3 citation statements)

References 26 publications

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“…Although the analytical performance in aforementioned technologies is satisfactory, specialized staff, lengthy procedures, and cumbersome pretreatment fail to satisfy the ever-increasing requirements of on-site surveillance concerns, especially for the emergency cases in poor economies and developing countries . Moreover, results of lacking relatively independent second signal-mediated built-in correction in the traditional single detection mode are susceptible to interference by the actual test environment. − In this sense, an accurate, rapid, and real-time detection method with a bimodal-type signal output pattern suitable for novice users and low-tech environments is an urgent objective within the actual point-of-care (POC) industry.…”

Section: Introductionmentioning

confidence: 99%

Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring

Liu,

Zhao,

Shu

et al. 2024

J. Agric. Food Chem.

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Immunochromatography (ICA) remains untapped toward enhanced sensitivity and applicability for fulfilling the nuts and bolts of on-site food safety surveillance. Herein, we report a fortified dual-spectral overlap with enhanced colorimetric/fluorescence dual-response ICA for on-site bimodal-type gentamicin (Gen) monitoring by employing polydopamine (PDA)-coated AuNPs (APDA) simultaneously serving as a colorimetric reporter and a fluorescence quencher. Availing of the enhanced colorimetric response that originated from the PDA layer, the resultant APDA exhibits less required antibody and immunoprobes in a single immunoassay, which facilitates improved antibody utilization efficiency and immuno-recognition in APDA-ICA. Further integrated with the advantageous features of fortified excitation and emission dual-spectral overlap for the Arg/ATT-AuNCs, this APDA-ICA with a “turn on/off” pattern achieves the visual limits of detection of 1.0 and 0.5 ng mL–1 for colorimetric and fluorescence patterns (25- and 50-fold lower than standard AuNPs-ICA). Moreover, the excellent self-calibration and satisfactory recovery of 79.03–118.04% were shown in the on-site visual colorimetric–fluorescence analysis for Gen in real environmental media (including real river water, an urban aquaculture water body, an aquatic product, and an animal byproduct). This work provides the feasibility of exploiting fortified dual-spectral overlap with an enhanced colorimetric/fluorescence dual response for safeguarding food safety and public health.

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Section: Introductionmentioning

confidence: 99%

Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring

Liu,

Zhao,

Shu

et al. 2024

J. Agric. Food Chem.

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“…38 However, the chaotic external environment is not conducive to the singlesignal experiment and colorimetric results after the catalytic reaction, resulting in inaccurate and insensitive detection. 39 Therefore, for a more accurate analysis, it is important to verify the results with a secondary signal response. 40 Some nanoparticles can convert the typical absorption signal of enzymelinked immunosorbent assay (ELISA) into a fluorescent signal, which is a feasible and promising strategy to improve the detection sensitivity.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Among them, dual-mode signal amplification attracted great interest because of its unique advantages. ,− The two most widely used output signals for the development of dual-mode sensors are colorimetric and fluorescence signals. − In colorimetric immunoassay, colored substances or enzymes are often labeled to detection antibodies as signal amplification . However, the chaotic external environment is not conducive to the single-signal experiment and colorimetric results after the catalytic reaction, resulting in inaccurate and insensitive detection . Therefore, for a more accurate analysis, it is important to verify the results with a secondary signal response .…”

Section: Introductionmentioning

confidence: 99%

Sensitive Dual-Signal ELISA Based on Specific Phage-Displayed Double Peptide Probes with Internal Filtering Effect to Assay Monkeypox Virus Antigen

Pang,

Wang,

Yuan

et al. 2024

Anal. Chem.

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The global spread of monkeypox has become a worldwide public healthcare issue. Therefore, there is an urgent need for accurate and sensitive detection methods to effectively control its spreading. Herein, we screened by phage display two peptides M4 (sequence: DPCGERICSIAL) and M6 (sequence: SCSSFLCSLKVG) with good affinity and specificity to monkeypox virus (MPXV) B21R protein. To simulate the state of the peptide in the phage and to avoid spatial obstacles of the peptide, GGGSK was added at the C terminus of M4 and named as M4a. Molecular docking shows that peptide M4a and peptide M6 are bound to different epitopes of B21R by hydrogen bonds and salt-bridge interactions, respectively. Then, peptide M4a was selected as the capture probe, phage M6 as the detection probe, and carbonized polymer dots (CPDs) as the fluorescent probe, and a colorimetric and fluorescent double-signal capture peptide/antigen/signal peptidedisplayed phage sandwich ELISA triggered by horseradish peroxidase (HRP) through a simple internal filtration effect (IFE) was constructed. HRP catalyzes H 2 O 2 to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue oxidized TMB, which can further quench the fluorescence of CPDs through IFE, enabling to detect MPXV B21R in colorimetric and fluorescent modes. The proposed simple immunoassay platform shows good sensitivity and reliability in MPXV B21R detection. The limit of detection for colorimetric and fluorescent modes was 27.8 and 9.14 pg/mL MPXV B21R, respectively. Thus, the established double-peptide sandwich-based dual-signal immunoassay provides guidance for the development of reliable and sensitive antigen detection capable of mutual confirmation, which also has great potential for exploring various analytical strategies for other respiratory virus surveillance.

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Propelling gold nanozymes: catalytic activity and biosensing applications

Huang,

Xiang,

et al. 2024

Anal Bioanal Chem

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Two birds with one stone: Gold “bridging” both nanozyme and immunosensor for sensitive double-response detection of T-2 toxin

Cited by 7 publications

References 26 publications

Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring

Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring

Sensitive Dual-Signal ELISA Based on Specific Phage-Displayed Double Peptide Probes with Internal Filtering Effect to Assay Monkeypox Virus Antigen

Propelling gold nanozymes: catalytic activity and biosensing applications

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