TWO APPARENTLY HEALTHY JAPANESE INDIVIDUALS OF TYPE MkMk HAVE ERYTHROCYTES WHICH LACK BOTH THE BLOOD GROUP MN AND Ss‐ACTIVE SIALOGLYCOPROTEINS

Tokunaga, Eiichi; Sasakawa, Shigeru; Tamaka, Kyoko; Kawamata, Hitoshi; Giles, Carolyn M.; Ikin, Elizabeth W.; Poole, Joyce; Anstee, David J.; Mawby, W. J.; Tanner, Michael

doi:10.1111/j.1744-313x.1979.tb00693.x

Cited by 67 publications

(34 citation statements)

References 30 publications

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“…Cells of the Leach phenotype lack 3 minor sialoglycoproteins and have abnormal shape. In contrast, abnormal'cell shape has not been reported for either En(a -) erythrocytes which lack the major sialoglycoprotein LY (glycophorin A) or homozygous Mk erythrocytes which lack sialoglycoproteins (Y and 6 (glycophorin B) [15,16]. The differences could be due to the fact that the 3 minor sialoglycoproteins P, Pl and y are normally bound to the cytoskeleton, whereas sialoglycoproteins a and 6 are not [17,18].…”

Section: Methodsmentioning

confidence: 99%

The phospholipid organisation in the membranes of McLeod and Leach phenotype erythrocytes

et al. 1985

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The phospholipid composition, the distribution of phospholipids over the two membrane layers as well as the phosphatidylcholine-specific transfer protein-mediated exchangeability of phosphatidylcholine from the membrane, has been investigated in two types of abnormal erythrocytes -the McLeod phenotype and the Leach phenotype. The acanthocytic McLeod cells appeared to have a normal phospholipid composition and distribution, but the exchangeability of phosphatidylcholine was found to be markedly enhanced. Unlike control erythrocytes, in which 75% of all of the phosphatidylcholine can be exchanged during an 8 h incubation, the McLeod cell showed a complete exchange of this phospholipid within the same time period. This obviously indicates an enhanced transbilayer mobility of phosphatidylcholine in the membrane of McLeod cells. Erythrocytes of the Leach phenotype showed an elliptocytic shape and increased osmotic fragility, but no abnormalities were observed as to the composition and organisation of the phospholipid complement of their membranes. Erythrocyte shape Erythrocyte membrane Phospholipid distribution Phosphatidylcholine exchange Osmotic fragility

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Section: Methodsmentioning

confidence: 99%

The phospholipid organisation in the membranes of McLeod and Leach phenotype erythrocytes

et al. 1985

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“…When BRIC 163 was used in immunoblotting exper iments with the electrophoretically-separated compo nents of normal and homozygous Mk red cell membranes, the results demonstrated that, whilst BRIC 163 specificai-ly identifies bands corresponding to 1x2, aS and a in normal red cell membranes, no binding to homozygous Mk membranes occurred ( fig. 2k, 1 Five homozygous Mk individuals from 3 apparently unre lated kindreds, 2 of which are Japanese, have now been described [2,3]. Of these, 4 are in apparently normal health, the fifth, the proposita in this study, has Hodgkin's disease.…”

Section: Biochemical Resultsmentioning

confidence: 99%

“…This antibody may be anti-EnaTS. Alterna tively, it may be the same as the antibodies present in the sera of the Japanese MkMk blood donor and his MkMk brother described by Tokunaga et al [2], which failed to agglutinate En(a -) cells but was shown to bind to them by absorption tests. Although absorption of the serum of the proposita with En(a -) cells did not reduce the titre with En(a + ) cells, this may be due to the other, trypsin-resis tant, antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…(1) membranes of 1-4. The band marked with an asterisk in track e was described in Tokunaga et al [2] and shown not to contain a or 5 by these authors.…”

Section: Sds-page Of Membranes From Normal and Homozygousmentioning

confidence: 99%

“…In 1979, Tokunaga et al [2] described a Japanese family with 2 brothers homozygous for Mk. The red cells of these Mk homozygotes were M -N -S -s -U-En(a -) Wr(a-b-).…”

Section: Introductionmentioning

confidence: 99%

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A Japanese Family with Two Sisters Apparently Homozygous for M^k

Okubo¹,

Daniels²,

Parsons³

et al. 1988

Vox Sanguinis

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Two Japanese sisters with consanguineous parents have M —N — En(a —) Wr(a — b — ) S — s — U— red cells and are therefore apparently homozygous for M^k; the third reported family with members of this genotype. The serum of the proposita (ORCMK) contained anti-En^aTS, anti-En^a FR and possibly anti-Wr^b, whereas the serum of her M^kM^k sister contained no atypical antibodies. Total absence of sialoglycoproteins α and δ from red cell membranes of an M^k homozygote was demonstrated by lactoperoxidase-catalysed radioiodination of accessible tyrosine residues with subsequent SDS polyacrylamide gel electrophoresis and autoradiography, and by use of a monoclonal antibody directed at the cytoplasmic portion of α-sialoglycoprotein.

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Molecular Evolution of Glycophorins

Wilder

2010

Encyclopedia of Life Sciences

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Humans and closely related apes have four glycophorin‐encoding genes, three of which form a small paralogous gene family. These genes encode sialoglycoproteins that localise to the plasma membrane of the red blood cell. Although the biological function of these proteins is not well understood, and their presence does not appear necessary for human viability or normal health, all four glycophorin‐encoding genes show evidence of accelerated evolution through positive natural selection. This selection is likely caused by a coevolutionary dynamic between glycophorins and Plasmodium falciparum receptors that use these molecules to gain entry into the red blood cell. Key Concepts: Gene duplication, followed by DNA sequence divergence and non‐homologous recombination, has shaped the GYPA‐GYPB‐GYPC gene family. Positive natural selection, likely mediated by Plasmodium falciparum , has caused accelerated amino acid evolution at all glycophorin‐encoding genes.

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TWO APPARENTLY HEALTHY JAPANESE INDIVIDUALS OF TYPE M^kM^k HAVE ERYTHROCYTES WHICH LACK BOTH THE BLOOD GROUP MN AND Ss‐ACTIVE SIALOGLYCOPROTEINS

Cited by 67 publications

References 30 publications

The phospholipid organisation in the membranes of McLeod and Leach phenotype erythrocytes

The phospholipid organisation in the membranes of McLeod and Leach phenotype erythrocytes

A Japanese Family with Two Sisters Apparently Homozygous for M^k

Molecular Evolution of Glycophorins

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