Two and Three Dimensional Imaging of Normal and Osteoarthritic Cartilage Microstructure with Optical Coherence Tomography

Herrmann, Juergen M.; Pitris, Costas; Bouma, Brett E.; Boppart, Stephen A.; Fujimoto, James G.; Brezinski, Mark E.

doi:10.1364/aoipm.1998.atud3

Cited by 77 publications

(127 citation statements)

References 4 publications

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“…These attributes translate to high-quality in vivo imaging with contrast-enhanced CT in a rabbit model. Hence, this method complements other imaging modalities being explored for cartilage imaging, including MR imaging (19,(28)(29)(30)(31)(32)(33), US (34,35), fluorescence (36), and optical coherence tomography (37)(38)(39). With regard to tissue-specific CT contrast agents (40)(41)(42)(43)(44), the results here provide further guidance for optimization of the contrast agent for characterizing cartilage in vivo.…”

Section: Experimental Studies: Ct Enhanced With High-affinity Cationimentioning

confidence: 76%

Contrast-enhanced CT with a High-Affinity Cationic Contrast Agent for Imaging ex Vivo Bovine, Intact ex Vivo Rabbit, and in Vivo Rabbit Cartilage

Stewart

Bansal²,

Entezari³

et al. 2013

Radiology

104

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Purpose:To quantify the affinity of a cationic computed tomography (CT) contrast agent (CA 4+ ) and that of an anionic contrast agent (ioxaglate) to glycosaminoglycans (GAGs) in ex vivo cartilage tissue explants and to characterize the in vivo diffusion kinetics of CA 4+ and ioxaglate in a rabbit model. Materials and Methods:All in vivo procedures were approved by the institutional animal care and use committee. The affinities of ioxaglate and CA 4+ to GAGs in cartilage (six bovine osteochondral plugs) were quantified by means of a modified binding assay using micro-CT after plug equilibration in serial dilutions of each agent. The contrast agents were administered intraarticularly to the knee joints of five New Zealand white rabbits to determine the in vivo diffusion kinetics and cartilage tissue imaging capabilities. Kinetics of diffusion into the femoral groove cartilage and relative contrast agent uptake into bovine plugs were characterized by means of nonlinear mixed-effects models. Diffusion time constants (t) were compared by using a Student t test. Results:The uptake of CA 4+ in cartilage was consistently over 100% of the reservoir concentration, whereas it was only 59% for ioxaglate. In vivo, the contrast material-enhanced cartilage reached a steady CT attenuation for both CA 4+ and ioxaglate, with t values of 13.8 and 6.5 minutes, respectively (P = .04). The cartilage was easily distinguishable from the surrounding tissues for CA 4+ (12 mg of iodine per milliliter); comparatively, the anionic contrast agent provided less favorable imaging results, even when a higher concentration was used (80 mg of iodine per milliliter). Conclusion:The affinity of the cationic contrast agent CA 4+ to GAGs enables high-quality imaging and segmentation of ex vivo bovine and rabbit cartilage, as well as in vivo rabbit cartilage.q RSNA, 2012 Supplemental material: http://radiology.rsna.org/lookup /suppl

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Section: Experimental Studies: Ct Enhanced With High-affinity Cationimentioning

confidence: 76%

Contrast-enhanced CT with a High-Affinity Cationic Contrast Agent for Imaging ex Vivo Bovine, Intact ex Vivo Rabbit, and in Vivo Rabbit Cartilage

Stewart

Bansal²,

Entezari³

et al. 2013

Radiology

104

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“…OCT and EME techniques are still immature [13,14]. Because of its microscopic resolution, high frequency ultrasound (HFU) may provide more information about the morphological, acoustical and mechanical properties of soft tissues [15,16,17,18].…”

mentioning

confidence: 99%

Ultrasound speed and attenuation in progressive trypsin digested articular cartilage

Huang

Sun

et al. 2011

Sci. China Life Sci.

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Subtle changes of articular cartilage (AC) can lead to tissue degeneration and even osteoarthritis (OA). The early degeneration of AC is closely related to a change in proteoglycans (PG) content. The observation of PG is therefore an appropriate way of studying OA and evaluating the degree of AC degeneration. In this study, 20 cartilage-bone samples were prepared from normal porcine femoral condyle cartilage and 10 samples were digested over 2 h using 0.25% trypsin solution. The dynamic process of PG-digestion was explored using a conventional A-mode ultrasound (US) experimental system with a 10 MHz center frequency. Quantitative acoustic parameters were calculated from ultrasonic radio-frequency echo signals and included US speed (USS), US amplitude attenuation coefficient (UAA) and broadband US attenuation coefficient (BUA). The experimental results showed that the conventional A-mode ultrasound is valuable for tracking the degree of PG-digestion. Histology also confirmed the validity of the ultrasound observations. For every AC sample, the degree of PG-digestion within a given time was different and was affected by individual differences. After two hours of degeneration, USS showed a mean decrease of 0.4% (P<0.05). UAA was significantly lower after a two-hour PG depletion period (from (2.45±0.23) to (2.28±0.41) dB mm 1 ).BUA showed no significant differences during this process. In conclusion, conventional ultrasound can provide useful information about trypsin-induced progressive PG depletion in AC and can reflect variations of PG content via the quantitative acoustic parameters USS and UAA. The results of this study may be used to identify an indirect indicator of cartilage matrix integrity and OA disease progression.

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“…Two areas of extensive PS-OCT investigation are the assessment of collagen in musculoskeletal tissue and in coronary arteries [6][7][8][9]. When collagen is organized in a normal, nonpathologic state, the tissue retains this component of birefringence and backscattering changes with depth.…”

Section: Introductionmentioning

confidence: 99%

Fossilized Teeth as a New Robust and Reproducible Standard for Polarization-Sensitive Optical Coherence Tomography

2013

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A clinical need exists for a cheap and efficient standard for polarization sensitive optical coherence tomography (PS-OCT). We utilize prehistoric fossilized teeth from the Megalodon shark and European horse as an unconventional, yet robust standard. Given their easy accessibility and the microstructural consistency conferred by the process of fossilization, they provide a means of calibration to reduce error from sources such as catheter bending and temperature changes. We tested the maximum difference in birefringence values in each tooth and found the fossilized teeth to be fast and repeatable. The results were compared to measurements from bovine meniscus, tendon, and destroyed tendon, which were verified with histology.

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Two and Three Dimensional Imaging of Normal and Osteoarthritic Cartilage Microstructure with Optical Coherence Tomography

Cited by 77 publications

References 4 publications

Contrast-enhanced CT with a High-Affinity Cationic Contrast Agent for Imaging ex Vivo Bovine, Intact ex Vivo Rabbit, and in Vivo Rabbit Cartilage

Contrast-enhanced CT with a High-Affinity Cationic Contrast Agent for Imaging ex Vivo Bovine, Intact ex Vivo Rabbit, and in Vivo Rabbit Cartilage

Ultrasound speed and attenuation in progressive trypsin digested articular cartilage

Fossilized Teeth as a New Robust and Reproducible Standard for Polarization-Sensitive Optical Coherence Tomography

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