Two actin‐interacting protein 1 isoforms function redundantly in the somatic gonad and are essential for reproduction in <i>Caenorhabditis elegans</i>

Ono, Kanako; Ono, Shoichiro

doi:10.1002/cm.21152

Cited by 20 publications

(19 citation statements)

References 57 publications

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“…This information suggests that selected oocytes might undergo apoptosis during oogenesis to ensure normal development of sister oocytes, consistent with evidence for some other ecdysozoans (Andux and Ellis, 2008; Buszczak and Cooley, 2000). Interestingly, transcriptional enrichment in the female also relates to actin and cytoskeletal activity, interpreted to be linked to the contractility in the ovary of O. dentatum , and consistent with the finding of non-striated actin filament networks in the myoepithelial sheath of the proximal ovary of C. elegans - which provides contractile forces essential for the transport of eggs in the uterus and “ovulation” (Ono and Ono, 2014). …”

Section: Gender-enriched Transcriptionsupporting

confidence: 70%

Cracking the nodule worm code advances knowledge of parasite biology and biotechnology to tackle major diseases of livestock

Tyagi

Joachim

Ruttkowski

et al. 2015

Biotechnology Advances

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Many infectious diseases caused by eukaryotic pathogens have a devastating, long-term impact on animal health and welfare. Hundreds of millions of animals are affected by parasitic nematodes of the order Stronglida. Unlocking the molecular biology of representatives of this order, and understanding nematode-host interactions, drug resistance and disease using advanced technologies could lead to entirely new ways of controlling the diseases that they cause. Oesphagostomum dentatum (nodule worm; superfamily Strongyloidea) is an economically important strongylid nematode of swine worldwide. The present article reports recent advances made in biology and animal biotechnology through the draft genome and developmental transcriptome of O. dentatum, in order to support biological research of this and related parasitic nematodes as well as the search for new and improved interventions. This first genome of any member of the Strongyloidea is 443 Mb in size and predicted to encode 25,291 protein-coding genes. Here, we review the dynamics of transcription throughout the life cycle of O. dentatum, describe double-stranded RNA interference (RNAi) machinery and infer molecules involved in development and reproduction, and in inducing or modulating immune responses or disease. The secretome predicted for O. dentatum is particularly rich in peptidases linked to interactions with host tissues and/or feeding activity, and a diverse array of molecules likely involved in immune responses. This research progress provides an important resource for future comparative genomic and molecular biological investigations as well as for biotechnological research toward new anthelmintics, vaccines and diagnostic tests.

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Section: Gender-enriched Transcriptionsupporting

confidence: 70%

Cracking the nodule worm code advances knowledge of parasite biology and biotechnology to tackle major diseases of livestock

Tyagi

Joachim

Ruttkowski

et al. 2015

Biotechnology Advances

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“…1), which is known to affect myosin II activity 35 and therefore a cell's ability to generate tension. Moreover, Wdr1 is essential for the assembly of contractile actin networks in the Caenorhabditis elegans myoepithelial sheath 36 , the ability of the mouse heart to contract 37 , and to recruit myosin II to the contractile ring during cytokinesis 10 . This prompted us to wonder whether Wdr1 might also regulate tension within the mammalian epidermis.…”

Section: Resultsmentioning

confidence: 99%

Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity

et al. 2015

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During mouse development, core planar cell polarity (PCP) proteins become polarized in the epidermal plane to guide angling/morphogenesis of hair follicles. How PCP is established is poorly understood. Here, we identify a key role for Wdr1 (also known as Aip1), an F-actin-binding protein that enhances cofilin/destrin-mediated F-actin disassembly. We show that cofilin and destrin function redundantly in developing epidermis, but their combined depletion perturbs cell adhesion, cytokinesis, apicobasal polarity and PCP. Although Wdr1 depletion accentuates single-loss-of-cofilin/destrin phenotypes, alone it resembles core PCP mutations. Seeking a mechanism, we find that Wdr1 and cofilin/destrin-mediated actomyosin remodelling are essential for generating or maintaining cortical tension within the developing epidermal sheet and driving the cell shape and planar orientation changes that accompany establishment of PCP in mammalian epidermis. Our findings suggest intriguing evolutionary parallels but mechanistic modifications to the distal wing hinge-mediated mechanical forces that drive cell shape change and orient PCP in the Drosophila wing disc.

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“…Defects in a host of other cytoskeletal components do lead to ovulation defects similar to the formin mutants (failed entry and broken oocytes), including mutation or knock-down of genes encoding tropomyosin (lev-11), members of the troponin complex (TnC pat-10, TnT mup-2, TnI tni-1, unc-27/tni-2), paramyosin (unc-15), muscle myosin heavy chains (myo-3, unc-54), nonmuscle myosin heavy and light chains (nmy-2 and mlc-4), b-integrin (pat-3), vinculin (deb-1), and AIP1 (unc-78, aipl-1) [Myers et al, 1996;Ono and Ono, 2004;Ono et al, 2007;Obinata et al, 2010;Ono and Ono, 2014;Ono and Ono, 2016]. However, these have all been implicated in regulating the contractions of the myoepithelial sheath cells, rather than defects of spermatheca function.…”

Section: Discussionmentioning

confidence: 99%

INF2‐ and FHOD‐related formins promote ovulation in the somatic gonad of C. elegans

et al. 2016

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Formins are regulators of actin filament dynamics. We demonstrate here that two formins, FHOD-1 and EXC-6, are important in the nematode Caenorhabditis elegans for ovulation, during which actomyosin contractions push a maturing oocyte from the gonad arm into a distensible bag-like organ, the spermatheca. EXC-6, a homolog of the disease-associated mammalian formin INF2, is highly expressed in the spermatheca, where it localizes to cell-cell junctions and to circumferential actin filament bundles. Loss of EXC-6 does not noticeably affect the organization the actin filament bundles, and causes only a very modest increase in the population of junction-associated actin filaments. Despite absence of a strong cytoskeletal phenotype, approximately half of ovulations in exc-6 mutants exhibit extreme defects, including failure of the oocyte to enter the spermatheca, or breakage of the oocyte as the distal spermatheca entrance constricts during ovulation. Loss of FHOD-1 alone has little effect, and we cannot detect FHOD-1 in the spermatheca. However, combined loss of these formins in double fhod-1;exc-6 mutants results in profound ovulation defects, with significant slowing of the entry of oocytes into the spermatheca, and failure of nearly 80% of ovulations. We suggest that EXC-6 plays a role directly in the spermatheca, perhaps by modulating the ability of the spermatheca wall to rapidly accommodate an incoming oocyte, while FHOD-1 may play an indirect role relating to its known importance in the growth and function of the egg-laying muscles.

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Two actin‐interacting protein 1 isoforms function redundantly in the somatic gonad and are essential for reproduction in Caenorhabditis elegans

Cited by 20 publications

References 57 publications

Cracking the nodule worm code advances knowledge of parasite biology and biotechnology to tackle major diseases of livestock

Cracking the nodule worm code advances knowledge of parasite biology and biotechnology to tackle major diseases of livestock

Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity

INF2‐ and FHOD‐related formins promote ovulation in the somatic gonad of C. elegans

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