Twinkle, twinkle little star: Photoswitchable fluorophores for super‐resolution imaging

Chozinski, Tyler J.; Gagnon, Lauren A.; Vaughan, Joshua C.

doi:10.1016/j.febslet.2014.06.043

Cited by 125 publications

(114 citation statements)

References 128 publications

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“…606 Another interest driving research into creating photomodulatable QDs is their utility in microscopy and specifically the potential applications they may have in realizing super-resolution microscopy. 607 In contrast to the examples just above, for these types of biological applications the QDs will have to be stable in aqueous buffers. The first example was presented two years after the initial publication of pcFRET by combining a 550 nm emitting CdSe/ ZnS QD conjugated to MBP, which had been previously modified with a PC spiropyran.…”

Section: Aptamermentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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Luminescent semiconductor quantum dots (QDs) are one of the more popular nanomaterials currently utilized within biological applications. However, what is not widely appreciated is their growing role as versatile energy transfer (ET) donors and acceptors within a similar biological context. The progress made on integrating QDs and ET in biological configurations and applications is reviewed in detail here. The goal is to provide the reader with (1) an appreciation for what QDs are capable of in this context, (2) how this field has grown over a relatively short time span, and, in particular, (3) how QDs are steadily revolutionizing the development of new biosensors along with a myriad of other photonically active nanomaterial-based bioconjugates. An initial discussion of QD materials along with key concepts surrounding their preparation and bioconjugation is provided given the defining role these aspects play in the QDs ability to succeed in subsequent ET applications. The discussion is then divided around the specific roles that QDs provide as either Förster resonance energy transfer (FRET) or charge/electron transfer donor and/or acceptor. For each QD-ET mechanism, a working explanation of the appropriate background theory and formalism is articulated before examining their biosensing and related ET utility. Other configurations such as incorporation of QDs into multistep ET processes or use of initial chemical and bioluminescent excitation are treated similarly. ET processes that are still not fully understood such as QD interactions with gold and other metal nanoparticles along with carbon allotropes are also covered. Given their maturity, some specific applications ranging from in vitro sensing assays to cellular imaging are separated and discussed in more detail. Finally a perspective on how this field will continue to evolve is provided.

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Section: Aptamermentioning

confidence: 99%

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Spillmann²,

et al. 2016

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“…Recent years have also seen the development of new synthetic and protein based fluorescent probes. These probes have improved photo-physical properties, are more membrane permeable, and extend further over the spectral range, enabling the detection of more distinct labels with higher localization precision and at higher molecular densities 233,234 . Some synthetic probes are now available in a caged, non-fluorescent form 233,235 that can be selectively activated with a UV light-source.…”

Section: Future Outlookmentioning

confidence: 99%

Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane

2017

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Lipids and the membranes they form are fundamental building blocks of cellular life, and their geometry and chemical properties distinguish membranes from other cellular environments. Collective processes occurring within membranes strongly impact cellular behavior and biochemistry, and understanding these processes presents unique challenges due to the often complex and myriad interactions between membrane components. Super-resolution microscopy offers a significant gain in resolution over traditional optical microscopy, enabling the localization of individual molecules even in densely labeled samples and in cellular and tissue environments. These microscopy techniques have been used to examine the organization and dynamics of plasma membrane components, providing insight into the fundamental interactions that determine membrane functions. Here, we broadly introduce the structure and organization of the mammalian plasma membrane and review recent applications of super-resolution microscopy to the study of membranes. We then highlight some inherent challenges faced when using super-resolution microscopy to study membranes, and we discuss recent technical advancements that promise further improvements to super-resolution microscopy and its application to the plasma membrane.

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“…[1][2][3] The power of such imaging methods has led to increased interest in identifying new types of dyes, opticallyactive materials, and nanoparticles that have enhanced photophysical properties suitable for multimodal, multiplexed, and super-resolution imaging. [4][5][6][7][8][9][10][11][12][13][14] Because fluorophores play such a critical role in understanding biological processes, it is somewhat surprising that most advances in small molecule dye technology today rely on structural modifications of scaffolds discovered over a century ago. [15] For example, the robust Janelia FluorÒ and some AlexaFluorÒ dyes are structurally modified versions of rhodamine scaffolds discovered 130 years ago.…”

Section: Introductionmentioning

confidence: 99%

Expanding the Chemical Space of Biocompatible Fluorophores: Nanohoops in Cells

White¹,

Yu²,

Kawashima³

et al. 2018

Preprint

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Abstract:The design and optimization of fluorescent molecules has driven the ability to interrogate complex biological events in real time. Notably, most advances in bioimaging fluorophores are based on optimization of core structures that have been known for over a century. Recently, new synthetic methods have resulted in an explosion of non-planar conjugated macrocyclic molecules with unique optical properties yet to be harnessed in a biological context. Herein we report the synthesis of the first aqueous-soluble carbon nanohoop (i.e. a macrocyclic slice of a carbon nanotube prepared via organic synthesis) and demonstrate its bioimaging capabilities in live cells. This work establishes the nanohoops as an exciting new class of macrocyclic fluorophores poised for further development as novel bioimaging tools.

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Twinkle, twinkle little star: Photoswitchable fluorophores for super‐resolution imaging

Cited by 125 publications

References 128 publications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications

Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane

Expanding the Chemical Space of Biocompatible Fluorophores: Nanohoops in Cells

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