Twenty years of thin-layer chromatography

Stahl, Egon

doi:10.1016/s0021-9673(00)85728-3

Cited by 75 publications

(65 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chromatograms were stained by the molibdate reagent after Stahl (19 Error rate is defined as the molar ratio of non-complementary to complementary nucleotides incorporated.…”

Section: Methodsmentioning

confidence: 99%

A possible mechanism responsible for the correction of transcription errors

Volloch¹,

Rits²,

Tumerman³

1979

Nucl Acids Res

View full text Add to dashboard Cite

Nucleoside triphosphate phosphohydrolase (NTPase) activity was found in a preparation of E. Coli RNA polymerase. This enzymatic activity is capaable of hydrolysing all four ribonucleoside triphosphates to the nucleoside diphosphates. However, during in vitro RNA synthesis directed by poly(dC) or poly(dT), only the non-complementary nucleoside triphosphate of the same heterocyclic class was hydrolysed. No incorporation of the non-complementary precursor into RNA could be detected in these experiments. When another RNA polymerase preparation, devoid of NTPase activity, was employed, there was no hydrolysis of any nucleoside triphosphate and significant incorporation of non-complemtary precursor into RNA was observed.These observations lead us to the conclusion that NTPase, acting in conjunction with RNA polymerase, has the function of correcting errors in transcription.

show abstract

“…Chromatograms were stained by the molibdate reagent after Stahl (19 Error rate is defined as the molar ratio of non-complementary to complementary nucleotides incorporated.…”

Section: Methodsmentioning

confidence: 99%

A possible mechanism responsible for the correction of transcription errors

Volloch¹,

Rits²,

Tumerman³

1979

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…From the apparent repellent effect from the on-farm and the in vitro results the plants were screened for the presence of pyrethrins which are known natural repellents present in plants (Shahba et al, 2011), as per the method described by Stahl (1969) Commercial pyrethrum was used as a standard. The developed chromatograms were viewed under short-wave UV light and then sprayed with p-anisaldehyde-sulphuric acid in methanol and subsequently heated until optimal colour development.…”

Section: Pyrethrins Detection Assaymentioning

confidence: 99%

Isolation of seselin from Clausena anisata (Rutaceae) leaves and its effects on the feeding and development of Lucilia cuprina larvae may explain its use in ethnoveterinary medicine

Mukandiwa¹,

Ahmed²,

Eloff³

et al. 2013

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Ethnopharmacological relevance: The leaves of Clausena anisata are used traditionally to expel maggots from wounds of animals in Zimbabwe. We have previously proved in the laboratory that the plant certainly affects the behaviour and growth of blowfly larvae. The objective of this study was to isolate and identify the active compounds responsible for this activity. Materials and Methods:The acetone extract of C. anisata leaf powder was separated by solvent-solvent partition into five fractions. The n-hexane fraction was the most active in the larvicidal assay and therefore subjected to open column chromatography on silica gel. Results:The isolated compound was identified by nuclear magnetic resonance (NMR) and mass spectroscopy (MS) as the pyranocoumarin, seselin, chemically known as 2,2-dimethylpyranocoumarin . It inhibited feed intake in the first and second instars of blowfly larvae at the minimum concentration tested of 1ppm resulting in significant lower mass pupae (13.5 ± 0.5 mg and 22.4 ± 0.4 mg for the first and second instar larvae respectively) compared to the solvent control group (26.19 ± 0.8 mg) (p < 0.05).Conclusions: This is the first report of the isolation of seselin from the leaves of C. anisata and the first report of the compound having an effect against blow fly larvae.

show abstract

“…2b shows the spectra of surfactant with no shoulder at 440 nm, confirming the presence of deoxyhexose (rhamnose). Detection of type of sugar by TLC analysis, revealed the presence of rhamnose as it developed green colored spots on spraying with anisaldehyde H 2 SO 4 reagent with R f value 0.62 identical to standard rhamnose sugar (R f = 0.63) (Stahl, 1969). The identification of rhamnose was further confirmed by co-chromatography.…”

Section: Resultsmentioning

confidence: 87%

“…The lipid components that determine the surfaceactive properties of the BS were detected by spotting the partially purified BS on silica gel 60 (F 254 Merck, Germany) plates in the following solvent systems: chloroform : methanol : acetic acid (85 : 15 : 5) for total lipids; chloroform : methanol : water (65 : 35 : 8) for phospholipids; chloroform : methanol : water (65 : 25 : 4) for glycolipids and petroleum ether : diethyl ether : acetic acid (90 : 10 : 1) for neutral lipids (Stahl, 1969).…”

Section: Chemical Nature Of Biosurfactantsmentioning

confidence: 99%

See 1 more Smart Citation

Production of biosurfactant by Nocardia otitidiscaviarum and its role in biodegradation of crude oil

Vyas

Dave

2011

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Nocardia otitidiscaviarum microbial type cultural collection 6471 isolated from oil contaminated Alang India seawater was examined for production of surface active compound. Isolate shows halos and α-heamolysis on cetyl trimethyl ammonium bromide and blood agar respectively indicating the production of biosurfactant. Biosurfactant was extracted by precipitation and was partially purified. Partially purified product was characterized by thin layer chromatography and Infra-red spectroscopy and was identified as glycolipid. Sugar present in glycolipid was rhamnose and hence, biosurfactant was quantified as rhamnose equivalent. Role of cell-surface hydrophobicity and emulsification activity in correlation with biosurfactant production was examined. Correlation between biosurfactant production, growth and crude oil degradation was also examined and showed positive correlation at significant level 0.001 and 0.01 respectively. Thus, this is a first report on a marine strain of Nocardia otitidiscaviarum microbial type culture collection 6471, which can be a potential candidate for restoration of oil contaminated marine environment.

show abstract

Twenty years of thin-layer chromatography

Cited by 75 publications

References 42 publications

A possible mechanism responsible for the correction of transcription errors

A possible mechanism responsible for the correction of transcription errors

Isolation of seselin from Clausena anisata (Rutaceae) leaves and its effects on the feeding and development of Lucilia cuprina larvae may explain its use in ethnoveterinary medicine

Production of biosurfactant by Nocardia otitidiscaviarum and its role in biodegradation of crude oil

Contact Info

Product

Resources

About