Twenty years of FISH-based translocation analysis for retrospective ionizing radiation biodosimetry

Grégoire, Éric; Roy, Laurence; Buard, Valérie; Delbos, M; Durand, Valérie; Martin-Bodiot, Cécile; Voisin, P.; Sorokine-Durm, I.; Vaurijoux, Aurélie; Voisin, Philippe; Baldeyron, Céline; Barquinero, Joan Francesc

doi:10.1080/09553002.2018.1427903

Cited by 25 publications

(23 citation statements)

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“…Further, information on past radiation exposure is critical without which retrospective radiation dose assessment may not be reliable. Gregoire et al [85] analyzed the blood samples of 42 patients with suspected past IR overexposure by FISH using a cocktail of three chromosomes (2, 4 and 12). Radiation dose estimated by chromosome translocation was significantly different from 0 Gy in 25 of the 42 cases and the lowest positive dose estimated was 0.3 Gy.…”

Section: Fish Based Detection Of Chromosome Translocations For Retrosmentioning

confidence: 99%

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Balajee¹

2018

obm genet

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The technique of in situ hybridization (ISH) using radioactively labeled DNA probes was first described in the late 1960s and early 1970s. The first use of fluorescence in situ hybridization (FISH) was reported in 1980s where RNA labeled with a fluorophore at the 3' end was used to detect specific DNA sequences. Since then, the technique has undergone various modifications for detecting single genes, chromosomes and whole genomes on various targets such as interphase nucleus, prematurely condensed chromosomes, metaphase chromosomes, fresh and paraffinized tissue sections. Although FISH is quite frequently used in clinical diagnostics, its use has recently been extended to the field of radiation biodosimetry where both stable (translocations) and unstable aberrations (dicentrics and rings) are detected with high resolution for estimating the absorbed radiation dose in humans after incidental, accidental or occupational exposure to ionizing radiation. This review summarizes the diverse applications of FISH in radiation biodosimetry that range from radiation dose estimation to prediction of deterministic (i.e. acute radiation syndrome severity) and stochastic (i.e. genetic mutations and cancer) effects in the affected human population.

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Section: Fish Based Detection Of Chromosome Translocations For Retrosmentioning

confidence: 99%

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Balajee¹

2018

obm genet

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“…Bu nedenle daha hızlı bölünen hücrelerin oluşturduğu dokular radyasyondan daha fazla etkilenir. Hücreler G0/G1 fazları ve mitoz sırasında radyasyona maruz kaldığında, kromozomal fragmanların yanlış düzenlenmesi nedeni ile kromozom aberasyonları meydana gelebilir (12,13).…”

Section: Radyasyon Ve Hücre Döngüsüunclassified

“…İyonize radyasyona maruz kalan hücrelerde; translokasyon, ring kromozom ve asimetrik kromozom değişimi gibi kromozom anomalilerinin görülme sıklığı, disentrik kromozom ile benzerdir. Translokasyonların değişmeden aynı şekilde sonraki hücrelere aktarılması nedeni ile translokasyon skorlaması retrospektif doz belirlenmesi için biyobelirteç olarak önerilmektedir (12)(13)(14).…”

Section: Radyasyon Ve Kromozomunclassified

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Cellular and Molecular Effects of Ionizing Radiation

Manisaligil

Yurt

2018

Düzce Tıp Fakültesi Dergisi

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Hastalıkların erken tanı ve tedavisinde, iyonize radyasyon içeren görüntüleme yöntemleri önemli rol oynarlar. Tanı ve tedavide, hastaya uygulanacak radyasyon dozunun mümkün olduğunca az tutulması ve radyasyondan koruyucu önlemler alınmasına rağmen hastada radyasyon hasarı oluşma olasılığı bulunmaktadır. Bu nedenle düşük ve/veya yüksek doz radyasyon uygulamaları, hücrede biyokimyasal ve moleküler sinyal yolaklarını aktive ederek, hücresel düzeyde etkiler oluşturmaktadır. Günümüzde radyasyon içeren uygulamaların sıklığı ve teknik parametrelerin yüksek değerlerde kullanılmasıyla hasta dozları ve buna bağlı doku hasarları görülme olasılığı giderek artmaktadır. Radyasyon hasarı ile ilgili çalışmalarda rapor edilen hücresel mekanizmaların başında, apoptozis ve hücre içi reaktif oksijen türleri (Reactive Oxygen Species, ROS) oluşumu gelmektedir. Apoptozis, radyoterapi uygulamalarında tümörü yok etmede hedeflenen bir sonuç iken tanısal amaçlı düşük doz uygulamalarda doku hasarını arttırıcı istenmeyen bir sonuçtur. Tanı ve tedavi amacıyla kullanılan radyasyonun, farklı dokular üzerinde erken ve geç etkileri bulunmaktadır. Düşük doz iyonize radyasyonun hücresel düzeyde etkileri, hangi moleküller ve/veya sinyal ileti yolakları üzerinden oluştuğu konusunda çok sayıda araştırma olmasına rağmen henüz tam olarak açıklanamamıştır. Radyasyon hasarlarına ait moleküler düzeyde yapılan çalışmalar, oluşabilecek hasarların erken düzeyde belirlenmesi ve sonrasında önleyici tedavilerin planlanabilmesi açısından çok önemlidir. Bu derleme, radyasyonun hücrede farklı sinyal ileti yolakları ve moleküller üzerinden yaptığı etkileri güncel literatüre bağlı olarak değerlendirmeyi amaçlamıştır. Anahtar kelimeler: Radyasyon; hücre; doku hasarı; apoptozis; reaktif oksijen türleri.

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“…Stable aberrations may persist in the body for years and tend to accumulate during a lifetime. Therefore, translocation analysis is used as a biomarker for evaluating the received doses of ionizing radiation from past or chronic exposure [13]. FISH seems to be more sensitive than conventional chromosome analysis as was shown in medical personnel and in the study of environmental air pollution impact [14][15][16][17].…”

mentioning

confidence: 99%

Different chromosome damage in lymphocytes of newly diagnosed gastrointestinal and breast cancer patients

Kadlčíková¹,

Musilová²,

Hradská³

et al. 2020

neo

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Structural chromosome aberrations are a predictive biomarker of cancer risk. Conventional chromosome analysis widely used for these purposes detects unstable chromosome aberrations that are eliminated during cell division. Stable aberrations that may persist in the body and tend to accumulate during a lifetime can be detected by fluorescence in situ hybridization (FISH). The aim of the study was to investigate the level of chromosome damage in newly diagnosed cancer patients and control subjects by FISH. Both groups of untreated cancer patients had increased frequency of aberrant cells. However, chromosome damage affected different cytogenetic endpoints. Stable translocations and cells with complex rearrangements were elevated in breast cancer patients whereas unstable chromosome aberrations (dicentric chromosomes and acentric fragments) were elevated in gastrointestinal cancer patients. These associations observed in nonsmokers were typically not pronounced in smokers (with the exception of dicentric chromosomes in gastrointestinal patients). Exposure to tobacco smoke increased aberrations in healthy controls but not in the cancer patients. Our study suggests an association between cancer and stable chromosomal rearrangements in breast cancer patients. Unstable aberrations elevated in gastrointestinal cancer patients may be at least partly ascribed to the exposure to diagnostic X-rays.

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Twenty years of FISH-based translocation analysis for retrospective ionizing radiation biodosimetry

Cited by 25 publications

References 50 publications

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Cellular and Molecular Effects of Ionizing Radiation

Different chromosome damage in lymphocytes of newly diagnosed gastrointestinal and breast cancer patients

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