Twenty years of amino acid determination using capillary electrophoresis: A review

Ta, Hai Yen; Collin, Fabrice; Perquis, Lucie; Poinsot, Véréna; Ong-Meang, Varravaddheay; Couderc, François

doi:10.1016/j.aca.2021.338233

Cited by 56 publications

(32 citation statements)

References 366 publications

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“…Usually, optimal electropherograms can be obtained when using one or more of the 9 separation modes of CE if the target analytes are introduced into the capillary at high concentrations. However, working with diluted samples or complex mixtures where the target analytes are found at subnanomolar concentrations poses a significant limitation for detection of these analytes via CE; overcoming the poor sensitivity of CE has remained to be a challenge for many investigators [123,[169][170][171][172][173][174][175][176][177][178][179][180]. We will therefore discuss immunoaffinity capillary electrophoresis, a method of CE that prevail over many of these limitations.…”

Section: Capillary Electrophoresis As a Useful Technology For The Separation Of Structurally Related Small Molecular-weight Substances Anmentioning

confidence: 99%

“…If IACE were to be incorporated in sample preparation prior to sample introduction into the capillary or microchip, the entire system could be automated, reduce analysis time, and overcome limitations in poor detection sensitivity. We will emphasize the advantages for the determination of proteoforms when compared with other methods described to enhance the analytical sensitivity of analytes, such as field-amplified sample stacking, large-volume sample stacking, pH-mediated stacking, isotachophoreris (ITP), stacking techniques in micellar electrokinetic chromatography (MEKC), soli-phase extraction, and tagging target analytes with various chromophores and detecting the derivatized substances with sensitive detectors [123,151,152,[173][174][175][176][177][178][179][180][181][182][183][184][185][186][187][188][189][190]. In some instances, IACE can be used in combination with other methods to enhance analytical sensitivity even further.…”

Section: Capillary Electrophoresis As a Useful Technology For The Separation Of Structurally Related Small Molecular-weight Substances Anmentioning

confidence: 99%

“…In general, affinity capillary electrophoresis is a broad term referring to the separation by capillary electrophoresis of substances that participate in specific or non-specific noncovalent affinity interactions during electrophoresis. The interacting molecules can be found in solution or can be immobilized to a solid support [123,146,[174][175][176][177][178][179][180][191][192][193][194][195][196][197][198][199][200][201][202][203]. Immunoaffinity capillary electrophoresis, is the method referred to when using immobilized affinity ligands or probes to capture one or more analytes found in simple or complex matrices.…”

Section: Advantages Of Immunoaffinity Capillary Electrophoresis For the Detection Of Proteoformsmentioning

confidence: 99%

“…To avoid confusion when referring to affinity capillary electrophoresis, we have defined the terminology as free-solution affinity capillary electrophoresis (FSACE) to the two methods of affinity capillary electrophoresis whose interactions are carried out in solution, and immobilized affinity capillary electrophoresis or immunoaffinity capillary electrophoresis (IACE) when the ligand or probes are immobilized to a surface within a portion of the capillary or channel. However, IACE not only uses antibodies or antibody fragments as highly selective capture agents, but also other biorecognition affinity reagents can act as ligands or probes, such as lectins, aptamers, metal-organic and other substances that have affinity for other analytes [122,[174][175][176][177][178][179][180][191][192][193][194][195][196][197][198][199][200][201][202][203].…”

Section: Advantages Of Immunoaffinity Capillary Electrophoresis For the Detection Of Proteoformsmentioning

confidence: 99%

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Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

Guzman¹,

Guzman

2021

Biomolecules

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Over the years, multiple biomarkers have been used to aid in disease screening, diagnosis, prognosis, and response to therapy. As of late, protein biomarkers are gaining strength in their role for early disease diagnosis and prognosis in part due to the advancements in identification and characterization of a distinct functional pool of proteins known as proteoforms. Proteoforms are defined as all of the different molecular forms of a protein derived from a single gene caused by genetic variations, alternative spliced RNA transcripts and post-translational modifications. Monitoring the structural changes of each proteoform of a particular protein is essential to elucidate the complex molecular mechanisms that guide the course of disease. Clinical proteomics therefore holds the potential to offer further insight into disease pathology, progression, and prevention. Nevertheless, more technologically advanced diagnostic methods are needed to improve the reliability and clinical applicability of proteomics in preventive medicine. In this manuscript, we review the use of immunoaffinity capillary electrophoresis (IACE) as an emerging powerful diagnostic tool to isolate, separate, detect and characterize proteoform biomarkers obtained from liquid biopsy. IACE is an affinity capture-separation technology capable of isolating, concentrating and analyzing a wide range of biomarkers present in biological fluids. Isolation and concentration of target analytes is accomplished through binding to one or more biorecognition affinity ligands immobilized to a solid support, while separation and analysis are achieved by high-resolution capillary electrophoresis (CE) coupled to one or more detectors. IACE has the potential to generate rapid results with significant accuracy, leading to reliability and reproducibility in diagnosing and monitoring disease. Additionally, IACE has the capability of monitoring the efficacy of therapeutic agents by quantifying companion and complementary protein biomarkers. With advancements in telemedicine and artificial intelligence, the implementation of proteoform biomarker detection and analysis may significantly improve our capacity to identify medical conditions early and intervene in ways that improve health outcomes for individuals and populations.

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Section: Capillary Electrophoresis As a Useful Technology For The Separation Of Structurally Related Small Molecular-weight Substances Anmentioning

confidence: 99%

Section: Capillary Electrophoresis As a Useful Technology For The Separation Of Structurally Related Small Molecular-weight Substances Anmentioning

confidence: 99%

Section: Advantages Of Immunoaffinity Capillary Electrophoresis For the Detection Of Proteoformsmentioning

confidence: 99%

Section: Advantages Of Immunoaffinity Capillary Electrophoresis For the Detection Of Proteoformsmentioning

confidence: 99%

See 2 more Smart Citations

Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

Guzman¹,

Guzman

2021

Biomolecules

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“…CZE is appreciated as a powerful analytical technique featuring high separation efficiency (hundreds of thousands to millions of theoretical plates), high substance amount/mass sensitivity (picomole to attomole range in nanoliter to picoliter injected volumes), and short time of analysis (typically 10–20 min). CZE represents a suitable complement and/or alternative to different modes of HPLC and possesses high application potential for analysis and characterization of amino acids [ 14 ], peptides [ 15 ], proteins [ 16 ], and other (bio)molecules [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Capillary and Free-Flow Zone Electrophoresis for Analysis and Purification of Antimicrobial β-Alanyl-Tyrosine from Hemolymph of Fleshfly Neobellieria bullata

et al. 2021

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The problem of a growing resistance of bacteria and other microorganisms to conventional antibiotics gave rise to a search for new potent antimicrobial agents. Insect antimicrobial peptides (AMPs) seem to be promising novel potential anti-infective therapeutics. The dipeptide β-alanyl-tyrosine (β-Ala-Tyr) is one of the endogenous insect toxins exhibiting antibacterial activity against both Gram-negative and Gram-positive bacteria. Prior to testing its other antimicrobial activities, it has to be prepared in a pure form. In this study, we have developed a capillary zone electrophoresis (CZE) method for analysis of β‑Ala‑Tyr isolated from the extract of the hemolymph of larvae of the fleshfly Neobellieria bullata by reversed-phase high-performance liquid chromatography (RP-HPLC). Based on our previously described correlation between CZE and free-flow zone electrophoresis (FFZE), analytical CZE separation of β‑Ala‑Tyr and its admixtures have been converted into preparative purification of β‑Ala‑Tyr by FFZE with preparative capacity of 45.5 mg per hour. The high purity degree of the β‑Ala‑Tyr obtained by FFZE fractionation was confirmed by its subsequent CZE analysis.

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Capillary Electrophoresis in Food Analysis

Castro‐Puyana,

Bernardo‐Bermejo,

Abad‐Gil

et al. 2024

Encyclopedia of Analytical Chemistry

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The development and application of faster and more powerful analytical methodologies in Food Science is essential to face the demands from regulatory agencies, official laboratories, and consumers. In this field, capillary electrophoresis (CE) is nowadays a well‐established separation technique for food analysis that possesses exceptional properties related to high analysis speed and separation efficiencies combined with the small samples and reagent requirements. Moreover, it is a very versatile technique since different CE modes can be employed for a broad range of applications and separation challenges. In fact, CE should be considered as the first option when dealing with highly polar, charged, or chiral analytes. In the past two decades, a high amount of research work has been published, demonstrating the high potential of CE to analyze different food components in a broad variety of food matrices. The aim of this article is to provide an overview of the use of CE for food analysis including a summary of the characteristics of the CE modes and the main detectors mostly employed in this field. Besides, a wide view of the latest applications of CE in food analysis (2021–2023) is also provided, demonstrating the exceptional possibilities of this analytical technique.

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Twenty years of amino acid determination using capillary electrophoresis: A review

Cited by 56 publications

References 366 publications

Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

Application of Capillary and Free-Flow Zone Electrophoresis for Analysis and Purification of Antimicrobial β-Alanyl-Tyrosine from Hemolymph of Fleshfly Neobellieria bullata

Capillary Electrophoresis in Food Analysis

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